Pea-barley intercrop N dynamics in farmers fields

Knowledge about crop performances in farmers’ fields provides a link between on-farm practice and re-search. Thereby scientists may improve their ability to understand and suggest solutions for the problems facing those who have the responsibility of making sound agricultural decisions. Nitrogen (N) availability is known to be highly heterogeneous in terrestrial plant communities (Stevenson and van Kessel, 1997), a heterogeneity that in natural systems is often associated with variation in the distri-bution of plant species. In intercropping systems the relative proportion of component crops is influenced by the distribution of growth factors such as N in both time and space (Jensen, 1996). In pea-barley intercrops, an increase in the N supply promotes the growth of barley thereby decreasing the N accumulation of pea and giving rise to changes in the relative proportions of the intercropped components (Jensen, 1996). The pres-sure of weeds may, however, significantly change the dynamics in intercrops (Hauggaard-Nielsen et al., 2001). Data from farmers’ fields may provide direct, spatially explicit information for evaluating the poten-tials of improving the utilisation of field variability by intercrops

Estimation of sample spacing in stochastic processes

Motivated by applications in electron microscopy, we study the situation where a stationary and isotropic random field is observed on two parallel planes with unknown distance. We propose an estimator for this distance. Under the tractable, yet flexible class of Lévy-based random field models, we derive an approximate variance of the estimator. The estimator and the approximate variance perform well in two simulation studies

Isotope shifts of the (3s3p)3^3P0,1,2_{0,1,2} - (3s4s)3^3S1_1 Mg I transitions

We report measurements of the isotope shifts of the (3s3p)$^3$P$_{0,1,2}$ - (3s4s)$^3$S$_1$ Mg I transitions for the stable isotopes $^{24}$Mg (I=0), $^{25}$Mg (I=5/2) and $^{26}$Mg (I=0). Furthermore the $^{25}$Mg $^3$S$_1$ hyperfine coefficient A($^3$S$_1$) = (-321.6 $\pm$ 1.5) MHz is extracted and found to be in excellent agreement with state-of-the-art theoretical predictions giving A($^3$S$_1$) = -325 MHz and B($^3$S$_1$) $\simeq 10^{-5}$ MHz. Compared to previous measurements, the data presented in this work is improved up to a factor of ten.Comment: 4 pages, 4 figures submitted to PR

New Algorithm for Parallel Laplacian Growth by Iterated Conformal Maps

We report a new algorithm to generate Laplacian Growth Patterns using iterated conformal maps. The difficulty of growing a complete layer with local width proportional to the gradient of the Laplacian field is overcome. The resulting growth patterns are compared to those obtained by the best algorithms of direct numerical solutions. The fractal dimension of the patterns is discussed.Comment: Sumitted to Phys. Rev. Lett. Further details at http://www.pik-potsdam.de/~ander

Prevalence and characterization of plasmids carrying sulfonamide resistance genes among <em>Escherichia coli</em> from pigs, pig carcasses and human

BACKGROUND: Sulfonamide resistance is very common in Escherichia coli. The aim of this study was to characterize plasmids carrying sulfonamide resistance genes (sul1, sul2 and sul3) in E. coli isolated from pigs and humans with a specific objective to assess the genetic diversity of plasmids involved in the mobility of sul genes. METHODS: A total of 501 E. coli isolates from pig feces, pig carcasses and human stools were tested for their susceptibility to selected antimicrobial. Multiplex PCR was conducted to detect the presence of three sul genes among the sulfonamide-resistant E. coli isolates. Fifty-seven sulfonamide-resistant E. coli were selected based on presence of sul resistance genes and subjected to conjugation and/or transformation experiments. S1 nuclease digestion followed by pulsed-field gel electrophoresis was used to visualize and determine the size of plasmids. Plasmids carrying sul genes were characterized by PCR-based replicon typing to allow a comparison of the types of sul genes, the reservoir and plasmid present. RESULTS: A total of 109/501 isolates exhibited sulfonamide resistance. The relative prevalences of sul genes from the three reservoirs (pigs, pig carcasses and humans) were 65%, 45% and 12% for sul2, sul1, and sul3, respectively. Transfer of resistance through conjugation was observed in 42/57 isolates. Resistances to streptomycin, ampicillin and trimethoprim were co-transferred in most strains. Class 1 integrons were present in 80% of sul1-carrying plasmids and 100% of sul3-carrying plasmids, but only in 5% of sul2-carrying plasmids. The sul plasmids ranged from 33 to 160-kb in size and belonged to nine different incompatibility (Inc) groups: FII, FIB, I1, FIA, B/O, FIC, N, HI1 and X1. IncFII was the dominant type in sul2-carrying plasmids (52%), while IncI1 was the most common type in sul1 and sul3-carrying plasmids (33% and 45%, respectively). Multireplicons were found associated with all three sul genes. CONCLUSIONS: Sul genes were distributed widely in E. coli isolated from pigs and humans with sul2 being most prevalent. Sul-carrying plasmids belonged to diverse replicon types, but most of detected plasmids were conjugative enabling horizontal transfer. IncFII seems to be the dominant replicon type in sul2-carrying plasmids from all three sources

Quinone-mediated extracellular electron transfer processes in ex situ biomethanation reactors

Redox mediators are used in a wide diversity of systems including biological ones. We investigated the effect of adding an artificial quinone (2,7-AQDS) as external redox molecule to an anaerobic digester system dominated by hydrogenotrophic methanogens. When oxidized AQDS was present, the methanogens diverted electrons from H2 to reduce AQDS instead of CO2. The AQDS reduction process was accompanied by a temporary CH4 inhibition, which was re-established several days after the full reduction of AQDS to AH2QDS. The presence of AQDS furthermore resulted in a community shift from Methanobacterium as the dominant methanogen to a more diverse community of methanogens. Protein expression profiles showed a shift in cofactor preference of the adapted community, as a potential response mechanism to AQDS inhibition. AH2QDS was only used as electron donor to a limited extent. Stable isotope incorporation experiments here indicated that the acetogen Acetoanaerobium used AH2QDS to reduce CO2 into acetate.</p