A COMPARATIVE STUDY OF THE ANTI-OXIDATIVE AND ANTI-DIABETIC POTENTIAL OF IN VITRO AND IN VIVO ROOT AND LEAF EXTRACTS OF WITHANIA SOMNIFERA ON STREPTOZOTOCIN INDUCED DIABETIC RATS

Objective: The present investigation explores the possibilities of using the in vitro and in vivo root and leaf extracts of Withania somnifera for anti-diabetic and anti-hyperlipidaemic effects on streptozotocin-induced diabetic rats.Methods: In vitro shoot cultures of Withania somnifera were raised by the axillary proliferation in nodal explants from a garden grown plant using Murashige and Skoog medium then in vitro raised roots and shoots were used for the anti-hyperglycemic and anti-hyperlipidaemic experiment. After 72 h of STZ administration, the fasting blood glucose levels were measured and the rats showing FBG level>220 mg/dl were considered to be diabetic and were used for the hyperglycemic study. In vitro and in vivo methanolic root and leaf extracts were orally administered daily to diabetic rats for eight weeks. After the treatment period, blood glucose and serum enzymes like aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total cholesterol, triglycerides, HDL-c high density lipoprotein-bound cholesterol, LDL-c low density lipoprotein-bound cholesterol, LDH, serum protein level, total phenolics and anti-oxidative analysis (DPPH and FRAP) were determined.Results: The levels of blood glucose, AST, ALT, ALP, LDH, HDL-c significantly increased by the use of in vitro methanolic root extracts compared to normal control rats. However, remarkable loss of total protein, albumin, albumin: globulin (A: G) ratio was reported in streptozotocin-induced diabetic rats by using in vitro root extracts. Methanolic in vitro root extract at the dose levels of 300 mg/kg body weight produced a significant decrease in fasting blood glucose (FBG) level by 102.65 with respect to initial fasting blood glucose level after 30 d of the treatment. In vitro root extract demonstrated highest DPPH and FRAP free radical scavenging activity, i.e. 86.55±1.77 and 48.87±2.55 than other extracts.Conclusion: It may be concluded that methanolic in vitro root extract W. somnifera at the dose (300 mg/kg) has more potent anti-hyperglycaemic activity than the other in vitro and in vivo extracts of leaf and root on streptozotocin induced diabetic rats and was also found to be similar in effect to that of the standard drug ‘Glibenclamide'

An evaluation of efficacy of Lichtenstein mesh hernioplasty in emergency settings: a study in tertiary care hospital in Odisha

Background: Despite the high incidence, the technical aspects of hernia repair continue to evolve making it the most common operations performed by general surgeons. Lichtenstein mesh hernioplasty repairs all hernias without distortion of the normal anatomy and with no suture line tension. This study was performed to evaluate the outcomes of Lichtenstein mesh herinioplasty in emergency inguinal hernia patients.Methods: A 84 patients were operated for complicated (obstructed irreducible) inguinal hernia. A follow-up period of 6 months using the Quantitative and Qualitative Measurement Instrument for evaluation of Lichtenstein hernioplasty outcomes was completed for 44 emergency patients.Results: The age incidence of the hernia patients in the study group was 40% (25-35 years) followed by 24% (15-25 years). The anatomical position of the hernia in the study group was to the right having a dominant percentage of 72% followed by left (24%) with none in bilateral. Early postoperative complications in mesh repair (Lichtenstein hernioplasty) comprising of factors like wound infection (10%), hematoma (5%), seroma (10%) was significantly lower compared to tissue repair with wound infection (20%), hematoma (5%) and seroma (25%). Further mesh rejection in Lichtenstein hernioplasty was 0% for the study population. Similarly, late postoperative complications in Lichtenstein hernioplasty comprising of factors like wound dehiscence (4%), neuralgia (27%) was significantly lower compared to tissue repair with wound dehiscence (27%), neuralgia (41%).Conclusions: The study revealed that the use of polypropylene prosthesis in the emergency setting (obstructed hernia) is safe and outcomes are satisfactory

An evaluation of efficacy of Lichtenstein mesh hernioplasty in emergency settings: a study in tertiary care hospital in Odisha

Background: Despite the high incidence, the technical aspects of hernia repair continue to evolve making it the most common operations performed by general surgeons. Lichtenstein mesh hernioplasty repairs all hernias without distortion of the normal anatomy and with no suture line tension. This study was performed to evaluate the outcomes of Lichtenstein mesh herinioplasty in emergency inguinal hernia patients.Methods: A 84 patients were operated for complicated (obstructed irreducible) inguinal hernia. A follow-up period of 6 months using the Quantitative and Qualitative Measurement Instrument for evaluation of Lichtenstein hernioplasty outcomes was completed for 44 emergency patients.Results: The age incidence of the hernia patients in the study group was 40% (25-35 years) followed by 24% (15-25 years). The anatomical position of the hernia in the study group was to the right having a dominant percentage of 72% followed by left (24%) with none in bilateral. Early postoperative complications in mesh repair (Lichtenstein hernioplasty) comprising of factors like wound infection (10%), hematoma (5%), seroma (10%) was significantly lower compared to tissue repair with wound infection (20%), hematoma (5%) and seroma (25%). Further mesh rejection in Lichtenstein hernioplasty was 0% for the study population. Similarly, late postoperative complications in Lichtenstein hernioplasty comprising of factors like wound dehiscence (4%), neuralgia (27%) was significantly lower compared to tissue repair with wound dehiscence (27%), neuralgia (41%).Conclusions: The study revealed that the use of polypropylene prosthesis in the emergency setting (obstructed hernia) is safe and outcomes are satisfactory