The combination of meltblown technology and electrospinning - The influence of the ratio of micro and nanofibers on cell viability

This study describes the production, testing and characterization of biodegradable scaffolds for bone tissue, which consist of the exact ratio of meltblown microfibers and nanofibers produced through the electrostatic field. All fibrous materials were produced from polycaprolactone. Three kinds of materials were prepared in the experiment with the same area density and with different well-defined ratio of microfibers as a mechanical component and nanofibers as a cells adherent component. All prepared materials showed optimum porosity of the inner structure for cell proliferation and in comparison to the materials with nanofibers they had good mechanical properties. Important structural properties and homogenity of each material were observed by electron microscopy and analyzed by image analysis. The effect of various ratios of microfibers and nanofibers on adhesion and proliferation of osteoblasts in-vitro was characterized. © 2016 Elsevier B.V. All rights reserved