Three-dimensional Migration of Neurites Is Mediated by Adhesion Site Density and Affinity

Three-dimensional neurite outgrowth rates within fibrin matrices that contained variable amounts of RGD peptides were shown to depend on adhesion site density and affinity. Bi-domain peptides with a factor XIIIa substrate in one domain and a RGD sequence in the other domain were covalently incorporated into fibrin gels during coagulation through the action of the transglutaminase factor XIIIa, and the RGD-dependent effect on neurite outgrowth was quantified, employing chick dorsal root ganglia cultured two- and three-dimensionally within the modified fibrin. Two separate bi-domain peptides were synthesized, one with a lower binding affinity linear RGD domain and another with a higher binding affinity cyclic RGD domain. Both peptides were cross-linked into fibrin gels at concentrations up to 8.2 mol of peptide/mol of fibrinogen, and their effect on neurite outgrowth was measured. Both two- and three-dimensional neurite outgrowth demonstrated a bi-phasic dependence on RGD concentration for both the linear and cyclic peptide, with intermediate adhesion site densities yielding maximal neurite extension and higher densities inhibiting outgrowth. The adhesion site density that yielded maximal outgrowth depended strongly on adhesion site affinity in both two and three dimensions, with lower densities of the higher affinity ligand being required (0.8-1.7 mol/mol for the linear peptide versus 0.2 mol/mol for the cyclic peptide yielding maximum neurite outgrowth rates in three-dimensional cultures)

THE U.S. PESTICIDE INDUSTRY: USAGE TRENDS AND MARKET DEVELOPMENT

Agribusiness, Crop Production/Industries,

THE U.S. FERTILIZER INDUSTRY: DEMAND TRENDS AND MARKET STRUCTURE

Agribusiness,

The crash involvement of interstate drivers in Queensland

Previous research has suggested that driving interstate is more hazardous than driving in one’s home state. However, the increased risk does not appear to be due to greater risk-taking by these drivers, but due to the greater distances they travel, particularly on rural roads, and associated problems such as fatigue. To further examine this issue, an analysis was undertaken of all reported crashes involving interstate drivers that occurred in Queensland between 1993 and 1998. During this period, interstate drivers represented 5% of all drivers involved in fatal and serious injury crashes in Queensland. The analysis indicated that the crashes involving interstate drivers were no more likely to involve factors such as alcohol, speeding, inattention/negligence or inexperience. Rather, the crash involvement patterns of interstate drivers appear to relate more to the type and location of their driving. For example, in Queensland interstate drivers are over-represented in crashes involving: open road driving; driver fatigue; the overturning and sideswiping of vehicles; and weekend travel. Interstate drivers were also more likely to be considered at fault for the crashes they were involved in, compared with local drivers