2 research outputs found
ANTITUMOR AND CYTOTOXIC ACTIVITY OF GINGER ESSENTIAL OIL (ZINGIBER OFFICINALE ROSCOE)
Objective: To evaluate the cytotoxicty and antitumor activity of ginger essential oil (GEO).Methods: Cytotoxicity towards Dalton's Lymphoma Ascites (DLA) and Ehrlich Ascites Carcinoma (EAC) cell lines were evaluated by trypan blue exclusion method. In vitro cytotoxicity of GEO to L929 cells in culture were checked by MTT assay. The antitumor activity of GEO was determined by using DLA cell line induced solid tumor and EAC cell line induced ascites tumor model in mice and its comparison with standard anticancer drug cyclophosphamide.Results: GEO showed potent in vitro cytotoxic activity against DLA and EAC cell lines. IC50 value for DLA cell line was 11 μg/ml and for EAC cell lines 18 μg/ml. The IC50 of GEO was found to be 41 μg/ml against the L929 cell lines and to Vero cells was found to be ˃100 ug/ml. The treatment with GEO (500 mg/kg and 1000 mg/kg body weight) significantly reduced the volume of solid tumor development by 54.4% and 62.4% respectively. The life span was increased up to 50% in 1000 mg/kg b. wt GEO treated ascites tumor induced animals.Conclusion: This indicates the significant in vitro cytotoxic and antitumor properties of GEO suggesting its potential use as an anticancer agent.Â
An evaluation of antioxidant, anti-inflammatory, and antinociceptive activities of essential oil from Curcuma longa. L
Objectives : This study was aimed to evaluate the chemical composition,
antioxidant potential in vitro and in vivo, anti-inflammatory, and
antinociceptive activity of turmeric oil. Materials and Methods :
Chemical analysis of turmeric oil was done by gas chromatography/mass
spectrometry. Antioxidant activities in vitro was done by six different
methods and in vivo antioxidant activity was determined by measuring
superoxide generation from macrophages treated with
phorbol-12-myristate-13-acetate (PMA) as well as determining
antioxidant level after feeding the oil orally for one month.
Anti-inflammatory activity was studied in mice using carrageenan,
dextran, and formalin. Antinociceptive activity was evaluated by using
acetic acid-induced writhing movement in mice. Results : The main
constituent of essential oil of turmeric was found to be ar-turmerone
(61.79%), curlone (12.48%), and ar-curcumene (6.11%). Turmeric oil was
found to have in vitro antioxidant activity and IC 50 for scavenging
superoxides, hydroxyl radicals, and lipid peroxidation were 135 mg/ml,
200 mg/ml, and 400 mg/ml, respectively. The ferric-reducing activity
for 50 mg of turmeric essential oil was found to be 5 mM.
Intraperitoneal administration of oil was found to inhibit PMA-induced
superoxide radicals elicited by macrophages. Oral administration of
turmeric oil for one month to mice significantly increased superoxide
dismutase, glutathione, and glutathione reductase enzyme levels in
blood and glutathione-S-transferase and superoxide dismutase enzymes in
liver. Turmeric oil showed significant reduction in paw thickness in
carrageenan, dextran-induced acute inflammation, and formalin-induced
chronic inflammation. The drug produced significant antinociceptive
activity (P < 0.001) at all doses studied. Conclusions : These
results demonstrated that turmeric oil has potential health benefits as
it can scavenge the free radicals and produce significant
anti-inflammatory and antinociceptive activities