17 research outputs found
Characteristics of B cells present in organized tumor-associated tertiary lymphoid structures in human breast cancer
Poster non publiéinfo:eu-repo/semantics/nonPublishe
Characterization and organization of infiltrating B-cells in human breast cancer
info:eu-repo/semantics/nonPublishe
Characterization of B cells infiltrating human breast cancer.
info:eu-repo/semantics/publishe
The composition and organization of lymphocytes infiltrating human breast cancer
Poster presentation selected for a poster walkinfo:eu-repo/semantics/publishe
CXCL13-producing follicular helper T cells in human breast cancer.
info:eu-repo/semantics/publishe
Characterization of B cells infiltrating human breast cancer and their presence in peritumoral tertiary lymphoid structures
1669info:eu-repo/semantics/publishe
CXCL13-producing extrafollicular Tfh-like CD4+ T cells in human breast cancer
info:eu-repo/semantics/publishe
A simple and rapid protocol to non-enzymatically dissociate fresh human tissues for the analysis of infiltrating lymphocytes.
The ability of malignant cells to evade the immune system, characterized by tumor escape from both innate and adaptive immune responses, isnow accepted as an important hallmark of cancer. Our research on breast cancer focuses on the active role that tumor infiltrating lymphocytesplay in tumor progression and patient outcome. Toward this goal, we developed a methodology for the rapid isolation of intact lymphoid cellsfrom normal and abnormal tissues in an effort to evaluate them proximate to their native state. Homogenates prepared using a mechanicaldissociator show both increased viability and cell recovery while preserving surface receptor expression compared to enzyme-digested tissues.Furthermore, enzymatic digestion of the remaining insoluble material did not recover additional CD45+ cells indicating that quantitative and qualitative measurements in the primary homogenate likely genuinely reflect infiltrating subpopulations in the tissue fragment. The lymphoidcells in these homogenates can be easily characterized using immunological (phenotype, proliferation, etc.) or molecular (DNA, RNA and/orprotein) approaches. CD45+ cells can also be used for subpopulation purification, in vitro expansion or cryopreservation. An additional benefitof this approach is that the primary tissue supernatant from the homogenates can be used to characterize and compare cytokines, chemokines,immunoglobulins and antigens present in normal and malignant tissues. This protocol functions extremely well for human breast tissues andshould be applicable to a wide variety of normal and abnormal tissues.The video component of this article can be found at http://www.jove.com/video/52392/info:eu-repo/semantics/publishe
Characterization of follicular helper CD4 T cells and B cells resident in peritumoral tertiary lymphocyte structures as specific markers for an immunological grade in breast cancer.
P5-01-02info:eu-repo/semantics/publishe