Free-living Heterotrophic Flagellates from Intertidal Sediments of Saros Bay, Aegean Sea (Turkey)

This is the first study of free-living heterotrophic flagellates in intertidal sediments of Saros Bay, Aegean Sea (Turkey). In order to contribute to an understanding of the geographic distribution of free-living marine heterotrophic flagellates, we investigated the diversity of heterotrophic flagellates occurring in the bay from 25th June 2010 to 10th October 2010. Thirty eight species from 30 genera of heterotrophic flagellates and one unidentifi ed taxon are reported with uninterpreted records based on light-microscopy. The records consist of one apusomonad, one cercomonad, two choanofl agellates, two cryptomonads, 12 euglenids, one heteroloboseid, one kathablepharid, three kinetoplastids, six stramenopiles, two thaumatomonads and seven of uncertain affinities. All of the morphospecies described here was previously reported elsewhere and appear to be cosmopolitan

The enhancer activity of long interspersed nuclear element derived microRNA 625 induced by NF-κB

Transposable elements (TEs) are DNA sequences that cut or introduced into the genome, and they represent a massive portion of the human genome. TEs generate a considerable number of microRNAs (miRNAs) are derived from TEs (MDTEs). Numerous miRNAs are related to cancer, and hsa-miRNA-625 is a well-known oncomiR derived from long interspersed nuclear elements (LINEs). The relative expression of hsa-miRNA-625-5p differs in humans, chimpanzees, crab-eating monkeys, and mice, and four primers were designed against the 3′UTR of GATAD2B to analyze the different quantities of canonical binding sites and the location of miRNA binding sites. Luciferase assay was performed to score for the interaction between hsa-miRNA-625 and the 3′UTR of GATAD2B, while blocking NF-κB. In summary, the different numbers of canonical binding sites and the locations of miRNA binding sites affect gene expression, and NF-κB induces the enhancer activity of hsa-miRNA-625-5p by sharing the binding sites

HAE-RAE Bench: Evaluation of Korean Knowledge in Language Models

Large Language Models (LLMs) trained on massive corpora demonstrate impressive capabilities in a wide range of tasks. While there are ongoing efforts to adapt these models to languages beyond English, the attention given to their evaluation methodologies remains limited. Current multilingual benchmarks often rely on back translations or re-implementations of English tests, limiting their capacity to capture unique cultural and linguistic nuances. To bridge this gap for the Korean language, we introduce HAE-RAE Bench, a dataset curated to challenge models lacking Korean cultural and contextual depth. The dataset encompasses six downstream tasks across four domains: vocabulary, history, general knowledge, and reading comprehension. Contrary to traditional evaluation suites focused on token or sequence classification and specific mathematical or logical reasoning, HAE-RAE Bench emphasizes a model's aptitude for recalling Korean-specific knowledge and cultural contexts. Comparative analysis with prior Korean benchmarks indicates that the HAE-RAE Bench presents a greater challenge to non-native models, by disturbing abilities and knowledge learned from English being transferred.Comment: Revised Erro

Full-length cDNA sequences from Rhesus monkey placenta tissue: analysis and utility for comparative mapping

<p>Abstract</p> <p>Background</p> <p>Rhesus monkeys (<it>Macaca mulatta</it>) are widely-used as experimental animals in biomedical research and are closely related to other laboratory macaques, such as cynomolgus monkeys (<it>Macaca </it><it>fascicularis</it>), and to humans, sharing a last common ancestor from about 25 million years ago. Although rhesus monkeys have been studied extensively under field and laboratory conditions, research has been limited by the lack of genetic resources. The present study generated placenta full-length cDNA libraries, characterized the resulting expressed sequence tags, and described their utility for comparative mapping with human RefSeq mRNA transcripts.</p> <p>Results</p> <p>From rhesus monkey placenta full-length cDNA libraries, 2000 full-length cDNA sequences were determined and 1835 rhesus placenta cDNA sequences longer than 100 bp were collected. These sequences were annotated based on homology to human genes. Homology search against human RefSeq mRNAs revealed that our collection included the sequences of 1462 putative rhesus monkey genes. Moreover, we identified 207 genes containing exon alterations in the coding region and the untranslated region of rhesus monkey transcripts, despite the highly conserved structure of the coding regions. Approximately 10% (187) of all full-length cDNA sequences did not represent any public human RefSeq mRNAs. Intriguingly, two rhesus monkey specific exons derived from the transposable elements of AluYRa2 (SINE family) and MER11B (LTR family) were also identified.</p> <p>Conclusion</p> <p>The 1835 rhesus monkey placenta full-length cDNA sequences described here could expand genomic resources and information of rhesus monkeys. This increased genomic information will greatly contribute to the development of evolutionary biology and biomedical research.</p

Helicobacter pylori infection combined with DENA revealed altered expression of p53 and 14-3-3 isoforms in Gulo−/− mice

AbstractUnlike most other mammals, human bodies do not have the ability to synthesize vitamin C inside of their own bodies. Therefore, humans must obtain vitamin C through daily diet. Gulo−/− mice strain is known with deficiency, in which vitamin C intake can be controlled by diet like human, and would be valuable for investigating the molecular mechanism of various diseases. In the present study, we established Gulo−/− mice model and investigated the differentially expressed proteins in stomach tissue of Gulo−/− mice after Helicobacter pylori-infected, and followed by DENA, using immunohistochemistry and proteomic approach. The results of immunohistochemistry analysis of stomach tissue showed that the tumor suppressor, p53 protein, expression was significantly decreased (p<0.05) but not messenger RNA (mRNA) transcriptional level, and 14-3-3ε, 14-3-3δ, Ki-67 and cleaved caspase 3 expressions were significantly increased (p<0.05) by H. Pylori infection, and followed by DENA treatment in Gulo−/− mice. Moreover, knockdown of 14-3-3 isoforms (14-3-3ε, 14-3-3σ, 14-3-3ζ and 14-3-3η) were significantly increased sub-G1 phase (characteristics of apoptosis) in AGS cells and, phenotypic changes like cell shrinkage, density and cleaved nuclei were also observed. Proteome analyses showed that 14-3-3σ, 14-3-3η, and tropomyosin alpha-1 chain were down-regulated, and Hspd1 protein and HSC70 were up-regulated after H. Pylori-infection, and followed by DENA. The combined results of immunohistochemistry and proteomic analysis suggest that H. pylori altered the p53 and 14-3-3 isoforms expression and DENA further enhanced the H. pylori effect, which might be involved in carcinogenesis and metastasis of gastric cancer on Gulo−/− mice