Sericin/Human Placenta-Derived Extracellular Matrix Scaffolds for Cutaneous Wound Treatment—Preparation, Characterization, <i>In Vitro</i> and <i>In Vivo</i> Analyses

Human placenta is loaded with an enormous amount of endogenous growth factors, thereby making it a superior biomaterial for tissue regeneration. Sericin is a naturally occurring silk protein that is extensively used for biomedical applications. In the present work, sericin and human placenta-derived extracellular matrix were blended and fabricated in the form of scaffolds using the freeze-drying method for cutaneous wound treatment. The prepared sericin/placenta-derived extracellular matrix (SPEM) scaffolds were characterized to determine their morphology, functional groups, mechanical strength, and antibacterial activity. Scanning electron microscopic analysis of the scaffolds showed smooth surfaces with interconnected pores. In vitro MTT and scratch wound assays performed using HaCaT cells proved the non-toxic and wound-healing efficacy of SPEM scaffolds. In vivo CAM assay using fertilized chick embryos proved the angiogenic potency of the scaffolds. Animal experiments using Wistar albino rats proved that the open excision wounds treated with SPEM scaffolds significantly reduced wound size with collagen deposition. These results confirm that SPEM scaffolds can serve as a promising biomaterial for tissue regeneration

Sericin/Human Placenta-Derived Extracellular Matrix Scaffolds for Cutaneous Wound Treatment&mdash;Preparation, Characterization, In Vitro and In Vivo Analyses

Human placenta is loaded with an enormous amount of endogenous growth factors, thereby making it a superior biomaterial for tissue regeneration. Sericin is a naturally occurring silk protein that is extensively used for biomedical applications. In the present work, sericin and human placenta-derived extracellular matrix were blended and fabricated in the form of scaffolds using the freeze-drying method for cutaneous wound treatment. The prepared sericin/placenta-derived extracellular matrix (SPEM) scaffolds were characterized to determine their morphology, functional groups, mechanical strength, and antibacterial activity. Scanning electron microscopic analysis of the scaffolds showed smooth surfaces with interconnected pores. In vitro MTT and scratch wound assays performed using HaCaT cells proved the non-toxic and wound-healing efficacy of SPEM scaffolds. In vivo CAM assay using fertilized chick embryos proved the angiogenic potency of the scaffolds. Animal experiments using Wistar albino rats proved that the open excision wounds treated with SPEM scaffolds significantly reduced wound size with collagen deposition. These results confirm that SPEM scaffolds can serve as a promising biomaterial for tissue regeneration

Haemostatic potency of sodium alginate/aloe vera/sericin composite scaffolds – preparation, characterisation, and evaluation

AbstractFabrication of haemostatic materials with excellent antimicrobial, biocompatible and biodegradable properties remains as a major challenge in the field of medicine. Haemostatic agents play vital role in protecting patients and military individuals during emergency situations. Natural polymers serve as promising materials for fabricating haemostatic compounds due to their efficacy in promoting hemostasis and wound healing. In the present work, sodium alginate/aloe vera/sericin (SA/AV/S) scaffold has been fabricated using a simple cost-effective casting method. The prepared SA/AV/S scaffolds were characterised for their physicochemical properties such as scanning electron microscope, UV–visible spectroscopy and Fourier transform infra-red spectroscopy. SA/AV/S scaffold showed good mechanical strength, swelling behaviour and antibacterial activity. In vitro experiments using erythrocytes proved the hemocompatible and biocompatible features of SA/AV/S scaffold. In vitro blood clotting assay performed using human blood demonstrated the haemostatic and blood absorption properties of SA/AV/S scaffold. Scratch wound assay was performed to study the wound healing efficacy of prepared scaffolds. Chick embryo chorioallantoic membrane assay carried out using fertilised embryos proved the angiogenic property of SA/AV/S scaffold. Thus, SA/AV/S scaffold could serve as a potential haemostatic healthcare product due to its outstanding haemostatic, antimicrobial, hemocompatible, biocompatible and angiogenic properties