Evaluation of In-Vitro Antioxidant Activity of Biochanin A

This study was aimed to determine the in vitro antioxidant potential of biochanin A (BCA), an isoflavone phytochemical, by using various free radical scavenging assays. The free radical scavenging activity of BCA was evaluated by various standardized assays such as 1, 1-Diphenyl-2-Picryl Hydrazyl (DPPH●), 2, 2’-Azinobis-3-Ethylbenzothiazoline-6-Sulfonic Acid (ABTS●+), Ferric Reducing Antioxidant Power (FRAP),nitric oxide scavenging activity, reducing ability, hydroxy radical activity, superoxide anion scavenging activity, hydrogen peroxide radical, metal ion chelating activity  and phosphomolybdenum  assay. Four different concentrations of BCA (5, 10, 20, 40, μg/ml) were taken for evaluating the scavenging activity and which were compared with the antioxidant activity of standard ascorbic acid (AA). BCA showed good free radical scavenging activity, which was calculated as IC50. IC50 value of BCA was also comparable to Ascorbic acid (AA).Whereas AA was used as a standard. The scavenging activity of BCA was significantly elevated in a dose dependent manner. The BCA was exhibited a highest scavenging activity than the standard. The results obtained in the present study revealed that the BCA is an excellent free radical scavenger with the activity similar to that of AA. Keywords: Biochanin A, Antioxidant, Free radicals, Nitric oxides and Ascorbic aci