Does Transit Service Reliability Influence Ridership?

This research focused on analyzing the association between transit service reliability indicators and ridership. Further, the effect of road network, demographic, socioeconomic, and land use characteristics on transit service reliability was analyzed. The analysis was conducted at a bus stop level. Bus arrival/departure and ridership data from the Charlotte Area Transit System (CATS) was obtained. The road network, demographic, socioeconomic, and land use characteristics were captured within 0.25-mile and 0.50-mile buffers. Pearson correlation analysis was conducted to understand the association between road network, demographic, socioeconomic, and land use characteristics and bus transit service reliability measures. The results show that bus transit service reliability has a substantial impact on ridership and is influenced by road network, demographic, socioeconomic, and land use characteristics within the bus stop vicinity. The findings help public transportation agencies to effectively utilize available resources, plan, and provide equitable services to all riders

Heparin affinity tag and application thereof

In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginine

Heparin affinity tag and applications thereof

In one aspect, affinity tags for recombinant protein purification are described herein which, in some embodiments, can mitigate or overcome disadvantages of prior affinity tag systems. In some embodiments, for example, affinity tags described herein permit efficient elution of desired recombinant proteins with simplified solution systems, such as alkali metal salt solutions. An affinity tag described herein comprises an amino acid sequence including a repeating amino acid unit of BXXXBXX, wherein B is an amino acid selected from the group consisting of histidine, lysine and arginine and X is an amino acid selected from the group consisting of amino acids other than histidine, lysine and arginin

Engineered FGF compositions and methods of use thereof

The present invention relates to the development of stable mutants of FGF-1 and FGF-2. In particular, it relates to novel engineered FGF-1 and FGF-2 polypeptides as well as polynucleotides, DNA constructs, and vectors encoding such polypeptides. In another aspect, pharmaceutical compositions and hydrogels including the disclosed polypeptides, polynucleotides, DNA constructs, and vectors are provided. In a still further aspect, methods of treating conditions using the compositions disclosed herein are provided

Modulation of Interleukin-12 activity in the presence of heparin

Glycosaminoglycans (GAGs), especially heparin and heparan sulfate (HS), modulate the functions of numerous cytokines. The aims of this multidisciplinary research were to characterize heparin binding to interleukin-12 (IL-12) and determine the mechanism(s) by which heparin influences IL-12 bioactivity. Heparin and HS were found to bind human IL-12 (hIL-12) with low micromolar affinity and increase hIL-12 bioactivity by more than 6-fold. Conversely, other GAGs did not demonstrate significant binding, nor did their addition affect hIL-12 bioactivity. Biophysical studies demonstrated that heparin induced only minor conformational changes while size-exclusion chromatography and small angle X-ray scattering studies indicated that heparin induced dimerization of hIL-12. Heparin modestly protected hIL-12 from proteolytic degradation, however, this was not a likely mechanism for increased cytokine activity in vitro. Flow cytometry studies revealed that heparin increased the amount of hIL-12 bound to cell surfaces. Heparin also facilitated hIL-12 binding and signaling in cells in which both hIL-12 receptor subunits were functionally deleted. Results of this study demonstrate a new role for heparin in modulating the biological activity of IL-12

Engineered FGF1 and FGF2 compositions and methods of use thereof

Engineered FGF1 and FGF2 polypeptides, polynucleotides encoding these polypeptides and DNA constructs, vectors and compositions including these engineered polypeptides are provided herein. The engineered FGF1 and FGF2 polypeptides are more stable than their wild-type counterparts and may be more effective at treating a variety of conditions that FGF1 and FGF2 are useful for treating such as wound healing

Does Transit Service Reliability Influence Ridership?

69A3551747127This research focused on analyzing the association between transit service reliability indicators and ridership. Further, the effect of road network, demographic, socioeconomic, and land use characteristics on transit service reliability was analyzed. The analysis was conducted at a bus stop level. Bus arrival/departure and ridership data from the Charlotte Area Transit System (CATS) was obtained. The road network, demographic, socioeconomic, and land use characteristics were captured within 0.25-mile and 0.50-mile buffers. Pearson correlation analysis was conducted to understand the association between road network, demographic, socioeconomic, and land use characteristics and bus transit service reliability measures. The results show that bus transit service reliability has a substantial impact on ridership and is influenced by road network, demographic, socioeconomic, and land use characteristics within the bus stop vicinity. The findings help public transportation agencies to effectively utilize available resources, plan, and provide equitable services to all riders