Effect of Date Palm Pollen Suspension on Ovarian Function and Fertility in Adult Female Rats Exposed to Lead Acetate

       This study present the effects of Date Palm Pollen grains (DPP) (phoenix dactylifera) on ovary function and fertility in adult  female rats exposed to lead acetate. Forty adult female albino rats were divided randomly into four equal groups. The first control  group was given orally (1 ml) distal water,(T1) group given orally 150 mg / kg .B.W. DPP ( 0.5ml),(T2) group given orally  (10) mg / kg. B.W. lead acetate (1 ml) ,(T3) group given daily by oral administration of both DPP150 mg/kg B.W. and 10 mg / kg.  B.W. lead acetate .all animals treated via gavages needle for 6 weeks.At the end of experiment blood were collected to determine serum LH & FSH  level .      Treated( T1)  group showed the increase levels of LH  were on significant importance at a level of < 0.05 in comparison with zero time of the same group and in comparison with control group . To contrast to treated(T1)  group, the level on LH showed a significantly decrease levels in animals on treated (T2) group exposure lead acetate at a dose rate of  10 mg / kg. B.W daily .  The significant decrease in LH were showed in comparison the value with that at zero day of the same group and at a time on experiment 14, 28, 42 day post exposure in comparison with control group and with (T1) group exposed to DPP. Animal on  treated (T3) group exposed of both DPP at 150 mg /kg. B. W. and lead acetate 10 mg / kg. B.W. for 6 weeks didn`t show any significant changes during the experiment.      Animals on( T1) group that exposed to DPP at a dose rate on 150 mg / k. B.W. for 6 weeks showed a significant increase FSH level in serum of exposed animal in comparison with zero time of the same group and in comparison with contrl group  . While those on (T2) group which exposed to lead acetate at a dose rate of 10 mg / kg. B.W. for 6 weeks showed a significant decrease in FSH level in serum on day 14 and 42 following the exposure the decrease in FSH level in serum may significant in comparison with zero day of same group and with level on T1 group and control group. Animal on group T3 didn`t show any significant changes during the experiment.            This study conclude that DPP has obvious improvement effect on fertility hormones of Adult  female rats exposed to lead acetate

Toxicopathological and biochemical effects of Carbon Tetrachloride CCl4 with residual accumulation in Liver of mice

Objective :-  The aims of the present study are to determine the toxicopathological and pathophysiological effects of carbon tetrachloride (CCl4), with measurement residual quantity of CCl4 in liver tissue by (HSGC) method.    Methodology :- 40 Swiss strain white mice were used, average weigh about 30 –32 g , divided into three groups, the T1 and T2 group were administrated with (100 and 200 mg/kg/body weight CCl4 respectively , given via intraperitoneal injection two does weekly for 40 day). While the 3rd group served as control. Clinical signs were reported during the course of the study, then at day 40 post treatment , all animal were sacrificed, blood were collected at 20 and 40 days of treatment period for biochemical test  and post mortem examination was done and any gross lesions were reported, liver section was taken for pathophysiological examination, also for measurement residual accumulated in tissue by Head-Space Gas Chromatographic analysis (HSGC) method.    Results :- All results showed that CCl4 caused significant increase of serum alanine aminotransferase and aspartate aminotransferase activity. Histopathological picture of liver, showed damage of liver parenchyma, disappear arrangements of hepatocyte, loss of hepatic cord, with congestion and dilatation of blood vessel also coagulative necrosis and apoptotic cells. Infiltration of inflammatory cells (macrophages &lymphocyte). Also high quantity from CCl4 was residual in hepatic tissue.    Conclusion :- The present study investigated that the CCl4 affected on the liver tissue, and causes histopathological lesion with elevation of liver enzymes specially ALT& AST , with accumulative effect in liver tissue after measurement by GC system. And the degree of influence depended on the concentration of the toxic dose .