Bacterial Colonization of Burn Wounds

The objective behind this work was to find out the bacteriological profile of post burn infections in wound. The study was carried out from December 2010 to February 2011 at the Burns Unit of Al –Kindy Hospital and Al-Yarmook Hospital in Baghdad. Sixty burn patients have been investigated for bacterial profile of burn wound infections. Specimens were collected in the form of wound swabs. The organisms were isolated and identified by standard microbiological methods. Antimicrobial susceptibility test has been done by ATB-PSE5 kit(BioMereiux). Pseudomonas aeruginosa 35(58.3 %) was found to be the most common isolate followed by Klebsiella pneumonae 10(16.6%), Staphylococcus aureus 7(10%). ,E.coli 3(5%), Proteus merabilis 1(1.6 %), others 2(3.3 %).and sterile cultures 2(3.3 %). Antibiotic susceptibility pattren for Pseudomonas aeruginosa isolates have shown high susptibility for Amikacin 30(85.0%), Pipracillin-Tazobactam 27(77.2%) Piperacillin 25(71.4%), Ciprofloxaqcin 25(71.4%) and Colistin 24(68.5%) and low susceptibility for Tobramycin 20(57.2%) Gentamycin 18(51.0%). Psudomonas was found to be resistant to most of the therapeutic agent

Microbiological Analysis on Tigris River Water in the Selected Sites in Baghdad Province, Iraq

Bacteriological analyses were carried out on Tigris River water while passing through Baghdad City the capital of Iraq. The Tigris River was selected in this study because this river supplies water for many sector of development like agriculture, industry, transportation, aquaculture, public water supply. Microbial pollution indicators such as - total count, total coliforms, fecal coliforms was studied, Samples were collected over a period of twelve months from September 2013 to August 2014. The results showed variation in the number and density of microbial indicators between month and seasons of study sites. A highest number of bacterial pollutants recorded through summer and spring seasons. The total coliform and Faecal coliform counts exceeding acceptable limits are indicative of pollution. to protect human health must reduce the  pollution of the river through control the levels of pollutants discharged into Tigris River and reduce dissemination of the coliform and fecal coliform bacteria. Keywords: Bacterial indicator, Water pollution, Tigris Rive

Prevalence of CTX-M Gene in Klebsiella Pneumonia Isolated from Surface Water of Tigris River within Baghdad Province

The present work was conducted to study the prevalence of klebsiella pneumonia in surface water which a part of feacal coliform bacteria that consider one of  microbial pollution indicators in water, also determined their antibiotic resistance and ESBL encoding gene using PCR with specific primers for the detection of CTX-M gene. The results showed a total of 40 isolates of klebsiella pneumonia were tested for the presence of the CTX-M gene by PCR, 87.5% were carrying this gene, and also isolates were resistant to various classes of antibiotics. Water and sewage are the ones, which have been identified as reservoirs of enteric bacteria for spread of resistance factors, wastewater treatment plant did not show a satisfactory efficacy in removing pathogenic microorganisms, allowing for the dissemination of multiresistant bacteria into the environment and this can result in routes of dissemination of multiresistant bacteria and their genes of resistance into the environment, thus contaminating water resources, and having serious negative impact on public health. Keywords: CTX-M, k. pneumoni

Isolation and Identification of Vibrio cholerae causes Epidemic cholera 2007 in Iraq by Rapid Method (Immunochromatographic one step rapid visual test) and comparing it with the traditional Bacterlogical Methods

This study was for searching for Cholera Bacteria serotype which causes epidemiology Cholera in the 2007 in a fast method which contains (Rapid Visual Test) (Crystal V.C.) which was used for the first time in Iraq to diagnosis of Cholera Bacteria & compared with the traditional bacteriology method. The Cholera disease is one of the most dangerous epidemiological diseases which lead to death with a percentage of (50 – 70) % in the severe cases for untreated patients . For this purpose, 100 samples of stool from the patients from a (13) hospitals in Baghdad Governorate in the period from August to the end of December. The Cholera was diagnosis in two methods, 1st method was the fast method using the nitrocellulose which is coated with anti-body of Cholera Bacteria .The results was compared with the 2nd method which depends on the cultural characteristics of the cultural media, also the bacteria was diagnosis using the biochemical inspects by the system of API 20E in addition to the using of antisera to specify serotype& sub-serotype ( Monovalent, Polyvalent(O1) . Also, the relation between the disease & the months in the year in which the disease appear was studied. The results show the ability to isolate & identifecate (78) isolate for the serotype (01) in the fast method ( Rapid Visual Test ) ( Crystal V-C ) , & after the bacteriology inspects on these (78) isolate the all isolates were belongs for Cholera Bacteria of the serotype (01) till the sub-serotype , all the (78) isolates were belongs to the sub-serotype (Inaba). The results show, the most infection was in September (36) , & October (22)