One Health Outreach Efforts in Responding to Harmful Algal Blooms Issues in Hotspot Regions

Background: Globally, harmful algal blooms (HABs) in freshwater are both a historical and an ongoing issue for human, animal, and ecosystem health and have dramatic impacts on local communities. Multiple taxa of cyanobacteria, including Microcystis, Planktothrix, and Anabaena species can produce cyanotoxins. Exposure to these toxins may cause mild to severe adverse health effects.Methods: An orchestrated effort was made by a team from multiple academic disciplines, public health agencies including the Centers for Disease Control and Prevention (CDC), and community stakeholders to engage the human and animal health communities to prepare for and respond to the emerging health threat of cyanotoxins from HABs.Results: Our public health practice approaches reached multiple targeted stakeholders from public health, human, and animal health sectors. The team also helped promote the newly established nationwide One Health Harmful Algal Bloom (OHHABs) initiative in a HABs hotspot in Ohio.Conclusion: Harmful algal blooms are a One Health issue demonstrating the interactions between animal, human, and ecological health. Environmental monitoring can provide early detection of environmental events, such as HABs, that pose both public health and ecological health threats. Public health surveillance can identify human and animal health events that may be linked to local environmental event

Correlation between Tick Density and Pathogen Endemicity, New Hampshire

To assess the endemicity of tick-borne pathogens in New Hampshire, we surveyed adult tick vectors. Pathogens were more prevalent in areas of high tick density, suggesting a correlation between tick establishment and pathogen endemicity. Infection rates in ticks correlated with disease frequency in humans

Potential Drivers for the Re-Emergence of Canine Leptospirosis in the United States and Canada

Canine leptospirosis is an important zoonotic disease in many countries. This review examines potential drivers for increased diagnoses of canine leptospirosis in the United States and Canada, using the epidemiologic triad of agent-environment-host as a template. Leptospira spp. are classified into more than 250 serovars, but in many laboratories only 6 are routinely tested for in serologic agglutination tests of canine sera. Leptospiral infections in dogs may potentially go undetected with unemployed or currently employed diagnostic methods. Disease transmission from infected reservoir hosts usually occurs via urine-contaminated environmental sources such as water. Direct contact between infected and susceptible individuals, environmental factors such as climate changes in temperature and/or rainfall, and increasing number and urbanization of reservoir hosts may greatly increase dog exposure risks. A dog’s lifestyle may influence exposure risk to leptospirosis, but vaccination based on proper identification of circulating serogroups dramatically reduces post-exposure infections. Regrettably, resistance to vaccination by veterinarians and dog owners leaves a large number of dogs at risk for this zoonotic disease

Household knowledge, attitudes and practices related to pet contact and associated zoonoses in Ontario, Canada

Abstract Background Many human infections are transmitted through contact with animals (zoonoses), including household pets. Although pet ownership is common in most countries and non-pet owners may have frequent contact with pets, there is limited knowledge of the public’s pet contact practices and awareness of zoonotic disease risks from pets. The objective of this study was to characterize the general public’s knowledge, attitudes and risks related to pet ownership and animal contact in southern Ontario, Canada. Methods A self-administered questionnaire was distributed to individuals at two multi-physician clinics in Waterloo, Ontario, Canada during 2010. A single adult from each household was invited to participate in the study. Results Seventy five percent (641/853) of individuals approached completed the questionnaire. Pet ownership and contact were common; 64% of participants had a pet in their household and 37% of non-pet owning households had a member with at least weekly animal contact outside the home. Pet ownership was high (55%) for households with individuals at higher risk for infections (i.e., Conclusions These results suggest that there is a need for accessible zoonotic disease information for both pet and non-owning households, with additional efforts made by veterinary, human and public health personnel. Immediate educational efforts directed toward households with individuals at higher risk to infections are especially needed.</p

Infectious agents in feral swine in Ohio, USA (2009-2015): A low but evolving risk to agriculture and public health

As highly mobile and prolific animals, feral swine threaten public and livestock health. To quantify these threats, we analyzed disease surveillance samples to estimate the prevalence of key pathogens and parasites in feral swine captured in Ohio. Between 2009 and 2015, samples from 205 feral swine were tested for up to 13 pathogens. Methicillin susceptible Staphylococcus aureus was cultured from 29 of 138 (21%) individuals and antibodies to Leptospira spp. (16/69; 23%), Toxoplasma gondii (11/76; 15%), Trichinella spiralis (4/69; 6%), hepatitis E virus (1/32; 3%), Brucella spp. (2/138; 1%), and influenza A virus (2/176; 1%) were also detected. Lungworms (Metastrongylus spp.) were frequently identified (46/55; 84%). Evidence of infection or exposure was not detected to Mycobacterium bovis, classical swine fever, pseudorabies, and porcine reproductive and respiratory syndrome. All positive Brucella and hepatitis E virus samples and 63% (10/16) of the positive Leptospira spp. samples came from individuals identified as illegal out-of-state feral swine introductions. Results indicated an overall low prevalence of pathogens in feral swine in Ohio; however, the importation of live feral swine from other states remained an important concern for pathogen introduction and spread. Key Words: Brucella, Leptospira, Staphylococcus, Toxoplasma, Trichinella, Zoonose

Inactivation of porcine epidemic diarrhea virus using heated water

Porcine epidemic diarrhea virus (PEDV) is a very contagious swine pathogen that spreads easily via the fecal-oral route, notably from contaminated fomites. The present study investigated heated water as a method for rapid thermal inactivation of PEDV. Cell-culture adapted PEDV was treated with water at varying temperatures and viral titers were measured at multiple time points post-treatment. Viable PEDV was not recovered after a ten second or longer treatment with water heated to ≥76 °C; however, PEDV nucleic acid was detected in all samples regardless of treatment. Hot water decontamination could be considered in settings where chemical disinfection is impractical

Tracking eastern equine encephalitis virus perpetuation in the northeastern United States by phylogenetic analysis

Epidemics and epizootics of eastern equine encephalitis virus (EEEV) occur sporadically in temperate regions where transmission is seasonal from late summer to early fall. These outbreaks may derive from virus that overwinters locally or perhaps results from reintroduction of virus from other sites. To evaluate these possibilities, we compared the phylogenetic relationships of EEEV isolates obtained from mosquitoes collected during statewide arbo-virus surveillance in Connecticut, in addition to isolates from concurrent outbreaks in southern New Hampshire and upstate New York. In Connecticut, viral isolates grouped into temporally discrete clades by year of isolation or over 2 years of sampling. Two or more clades arose in 2000, 2001, 2003, 2004, and 2006, possibly the result of separate introduction events into the state, whereas viruses from upstate New York and New Hampshire segregated into single clades that persisted for 2 or more years. New Hampshire viruses shared recent common ancestry to those isolated in Connecticut suggesting viral dispersal among these regions. These results provide additional evidence for independent episodes of EEEV overwintering in northern foci. Copyright © 2008 by The American Society of Tropical Medicine and Hygiene