DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension. This procedure is simpler than previously reported methods

Ayadi, Ali

Jarboui, Mohammed Ali

Mseddi, Fatma

Sellami, Amira

Sellami, Hayet

Crossref

Directory of Open Access Journals

PubMed Central

English

al: Comparação de três protocolos de extração de DNA a partir de tecido fixado em formol e incluído em parafina.

et al: Comparison of different methods for extraction of DNA of fungal pathogens from cultures and blood.

et al: Identification of Fusarium species involved in human infections by 28S rRNA gene sequencing.

Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region (ITS2). BMC Microbiol

Rapid methods to extract DNA and RNA from Cryptococcus neoformans. FEMS Yeast Res

Takuma H: DNA fingerprinting patterns of Candida species using Hinf1 endonuclease.

TG: Rapid identification of pathogenic fungi directly from cultures by using multiplex PCR.

A rapid and easy method for the DNA extraction from Cryptococcus neoformans