Issue Framing in Online Discussion Fora

In online discussion fora, speakers often make arguments for or against something, say birth control, by highlighting certain aspects of the topic. In social science, this is referred to as issue framing. In this paper, we introduce a new issue frame annotated corpus of online discussions. We explore to what extent models trained to detect issue frames in newswire and social media can be transferred to the domain of discussion fora, using a combination of multi-task and adversarial training, assuming only unlabeled training data in the target domain.Comment: To appear in NAACL-HLT 201

Управление работой персонала «Suning Commerce Group Co»

Объектом исследования является компания "Suning Commerce Group Co". Предметом исследования является управление работой персонала "Suning Commerce Group Co". Цель работы – разработать рекомендации по повышению эффективности работы персонала "Suning Commerce Group Co". Актуальность заключается в значимости процесса управления персоналом для организации. Управление персоналом в компании является важной и ключевой составляющей всей организации"Suning Commerce Group Co" путем реализации рекомендаций по повышению эффективности работы персонала.The object of the study is the company "Suning Commerce Group Co". The subject of the study is the management of the work of the staff of Suning Commerce Group Co. The purpose of the work is to develop recommendations for increasing the efficiency of the work of the staff of Suning Commerce Group Co. The relevance lies in the importance of the process of personnel management for the organization. Personnel management in the company is an important and key component of the entire organization of "Suning Commerce Group Co" by implementing recommendations to improve staff performance

Development and characterization of novel reversibly switchable red fluorescent proteins with opposing switching modes

Since the discovery of the green fluorescent protein (GFP) from the jellyfish Aequorea victoria, a multitude of fluorescent proteins have been derived from the original GFP and its homologs by protein engineering. Their characteristics were especially adapted to the requirements of different types of live-cell fluorescence microscopy. This includes super-resolution microscopy techniques, which overcome the diffraction barrier by distinguishing fluorophores based on different molecular states. Fluorescent proteins in the subclass of reversibly switchable fluorescent proteins (RSFPs) can be switched from a fluorescent to a non-fluorescent state and vice versa by light of certain wavelengths. These RSFPs can be utilized as fusion tags in reversible saturable optical fluorescence transition (RESOLFT) microscopy to achieve diffraction-unlimited resolution. Compared to the green-emitting RSFPs, the application of red-emitting RSFPs benefits from the longer excitation and switching wavelengths utilized and the associated less phototoxicity. However, the number of red RSFPs applicable for live-cell RESOLFT microscopy is limited until now and the available red RSFPs exhibit undesirable characteristics like dimerization tendency. This work presents the development of novel red RSFPs for RESOLFT microscopy. In order to identify superior red RSFPs, a microscopic screening of mutant libraries expressed in E. coli colonies was employed. To additionally facilitate the screening for improved fluorescent proteins in a mammalian expression system, a monoclonal Bxb1 landing pad cell line was generated using CRISPR/Cas9 genome engineering. Since the application of these Bxb1 landing pad cells enables the expression of only a single mutant from a transfected library in each cell, this strategy was suitable to screen for red RSFPs with higher brightness and offers a large potential to establish further screening approaches. By semi-rational design and screening, novel red RSFPs with opposing switching modes were generated from bright conventional red fluorescent proteins. Three positive-switching RSFPs were developed on the basis of mRuby2 and two negative-switching RSFPs were derived from mScarlet. All of these five RSFPs are monomeric, feature low residual fluorescence in the off-state (below 5 %), and are among the red RSFPs with the highest molecular brightness known today. The characterization of their performance as fusion tag in transient and stable expressions suggest that the novel red RSFPs are suitable for live-cell microscopy. The quantum yields of the switchable mScarlet variants are the highest determined for red RSFPs to date and the switching kinetics of the new negative-switching RSFPs exceeded those of the recently published rsFusionRed2 and rsFusionRed3. In addition, the generated RSFPs based on mRuby2 outperformed rsCherry in all analyzed switching characteristics and represent the only red fluorescent and positive-switching candidates for live-cell RESOLFT microscopy. A resolution beyond the diffraction limit was demonstrated utilizing one of the switchable mRuby2 variants in a proof of concept experiment

Verkennend onderzoek langs de Witte Nete (provincie Antwerpen): een geomorfologische benadering van archeologisch-landschappelijk diagnostisch onderzoek in de Kempen

The valley of the Kleine Nete river is archaeologically a poorly documented region, primarily due to lack of archaeological surveys. This lack of knowledge stands in the way of developing efficient archaeological management strategies in light of planning- or development schemes. When an advice was asked for drawing up a basin management plan for the Nete basin, we decided for a period from end 2004 to march 2005 to focus attention within the department of the Central Archaeological Inventory (CAI) of the Flemish Heritage Institute on this region. For this purpose the data in the CAI was revised, a number of amateur archaeologists active in the region were contacted and thus some new findspots were recorded, and a sample fieldwalking survey was executed with the main upper course of the Kleine Nete (the Witte Nete) as central focuspoint. This survey is the subject of this article. Our aims were to 1) collect primary archaeological data, and 2) develop a methodology for regional diagnostic research and evaluation mapping taking into account the unknown archaeological heritage, without using the classic predictive modelling strategies. For this purpose the following steps were undertaken: 1) Creation of a basic geomorphological map of the area 2) Archaeological fieldwalking survey, aiming to equally sample all geomorphological units (if possible) 3) Comparing the results from steps 1 & 2 and identification of patterns 4) Augering survey to test the geomorphological model and assess the physical preservation of landscape elements 5) Interpretation and modelling preservation- and research potential. To develop the basic geomorphological map a number of sources were used: quaternary geological maps, soil maps, recent and historical topographical maps, aerial photographs, and the digital terrain model of Flanders. This last instrument was developed very recently (2004), and is a dtm covering the whole of Flanders. The basic notion behind the research was to integrate and interpret the landscape and archaeological data in one matrix, as a result of intertwined depositional and post-depositional processes. The result is a model of preservation potential and research possibilities. Rare landscape elements such as late glacial dune ridges, palaeochannels, etc. were identified and mapped, and it was possible to assess the historical and subrecent erosion processes in developing a general preservation model with the identification of strongly physically degraded areas. This approach also identified a number of important gaps in our geomorphological knowledge: primarily the phasing in the evolution and stratigraphy of the Holocene valleys, and the internal morphology of the Holocene dune areas. The archaeological artefacts collected during the fieldwalking survey were interpreted and assessed in light of the identified post-depositional processes and geomorphological units. In this interpretation distributional patterns could be distinguished which can be ascribed on the one hand to preferences in landuse patterns in different periods, on the other hand resulting from post- depositional processes, finally to the data collection strategy (fieldwalking). When comparing the patterns discovered during this study with archaeological prediction models used in the region, we had come to the conclusions that: 1) The available sources for the creation of good prediction models (in the classic, processual meaning of the term) are insufficient and lack detail 2) The classic prediction models are strongly simplified documents, taking into account only a minor number of parameters 3) The predicted patterns in these models, when applied to our study, are very unaccurate. On the basis of the resulting model pro-active strategies can be developed which include research strategies to fill in our gaps in the knowledge, and further evaluation and preservation strategies for the identified well preserved areas. Zones of rare preservation potential can be detected and in a next phase evaluated. In light of development schemes appropriate preventive measures can be formulated for each geomorphological unit. The research here described had mainly a methodological focus. Despite the small scale of the research we succeeded in creating a basic model for archaeological regional diagnostics for the Campine area, which is mainly based on geo-archaeological approaches. This model will be further refined in the future, attempting to fill in the gaps in the geomorphological knowledge, and gathering more basic data with additional surveys in the Campine area

Prospects for high-resolution microwave spectroscopy of methanol in a Stark-deflected molecular beam

Recently, the extremely sensitive torsion-rotation transitions in methanol have been used to set a tight constraint on a possible variation of the proton-to-electron mass ratio over cosmological time scales. In order to improve this constraint, laboratory data of increased accuracy will be required. Here, we explore the possibility for performing high-resolution spectroscopy on methanol in a Stark-deflected molecular beam. We have calculated the Stark shift of the lower rotational levels in the ground torsion-vibrational state of CH3OH and CD3OH molecules, and have used this to simulate trajectories through a typical molecular beam resonance setup. Furthermore, we have determined the efficiency of non-resonant multi-photon ionization of methanol molecules using a femtosecond laser pulse. The described setup is in principle suited to measure microwave transitions in CH3OH at an accuracy below 10^{-8}

Verslagen van archeologische toevalsvondsten uit 2020 waar geen verder onderzoek nodig of mogelijk was

Wanneer iemand buiten de context van een archeologisch onderzoek per toeval archeologische objecten of een archeologische site vindt, moet hij dit melden aan het agentschap Onroerend Erfgoed1. Dit gebeurde 143 maal in 2020. 19 van deze meldingen leidden tot een archeologische opgraving. In verschillende situaties is een onderzoek met ingreep in de bodem echter niet nodig. Het archeologisch onderzoek beperkt zich dan tot een terreinevaluatie en het registreren van de gemelde vondsten. Dit rapport bevat een selectie van archeologisch relevante toevalsvondsten zonder bodemingreep uit 2020. Deze willekeurige selectie toont aan dat archeologische terreinevaluaties beperkt in oppervlakte, ook interessante en relevante archeologische kenniswinst kunnen opleveren

The first-generation Daphnia magna linkage map

<p>Abstract</p> <p>Background</p> <p><it>Daphnia magna </it>is a well-established model species in ecotoxicology, ecology and evolution. Several new genomics tools are presently under development for this species; among them, a linkage map is a first requirement for estimating the genetic background of phenotypic traits in quantitative trait loci (QTL) studies and is also very useful in assembling the genome. It also enables comparative studies between <it>D. magna </it>and <it>D. pulex</it>, for which a linkage map already exists.</p> <p>Results</p> <p>Here we describe the first genetic linkage map of <it>D. magna</it>. We generated 214 F2 (intercross) clonal lines as the foundation of the linkage analysis. The linkage map itself is based on 109 microsatellite markers, which produced ten major linkage groups ranging in size from 31.1 cM to 288.5 cM. The total size of this linkage map extends to 1211.6 Kosambi cM, and the average interval for the markers within linkage groups is 15.1 cM. The F2 clones can be used to map QTLs for traits that differ between the parental clones. We successfully mapped the location of two loci with infertility alleles, one inherited from the paternal clone (Iinb1) and the other from the maternal clone (Xinb3).</p> <p>Conclusions</p> <p>The <it>D. magna </it>linkage map presented here provides extensive coverage of the genome and a given density of markers that enable us to detect QTLs of moderate to strong effects. It is similar in size to the linkage map of <it>D. pulex</it>.</p

The structure of academic self-concept. A methodological review and empirical illustration of central models

The structure of academic self-concept (ASC) is assumed to be multidimensional and hierarchical. This methodological review considers the most central models depicting the structure of ASC: a higher-order factor model, the Marsh/Shavelson model, the nested Marsh/Shavelson model, a bifactor representation based on exploratory structural equation modeling, and a first-order factor model. We elaborate on how these models represent the theoretical assumptions on the structure of ASC and outline their inherent psychometric properties. We analyzed these models using a data set of German 10th-grade students (N = 1,232) including a wide range of domain-specific ASCs as well as general ASC. The correlations among ASCs and between ASCs and academic achievement varied depending on the structural model used. We conclude with discussing recommendations for research purposes and advantages and limitations of each ASC model. Our approach may also guide research on other affective or motivational constructs (e.g., academic anxiety or interest). (DIPF/Orig.