5 research outputs found
Clusterin expression in elastofibroma dorsi
Background: Elastofibroma dorsi is a benign
soft tissue lesion composed of abnormal elastic fibers.
Degenerated elastic fibers in skin and liver are
associated with clusterin, an apoprotein that shares
functional properties with small heat shock proteins. We
evaluated the staining pattern and possible role of
clusterin in elastofibroma dorsi. Material and methods:
Twenty-one subcutaneous elastofibromas from the
scapular region were evaluated with Elastica van Gieson
and Orcein stains, immunohistochemically with
antibodies to clusterin, smooth muscle actin, S-100,
vimentin and CD34 and correlated with clinical data
with respect to physical trauma. Results: Clusterin
correlated with the staining pattern of Elastica van
Gieson and labelled abnormal broad coarse fibrillar and
globular elastic fibers in all elastofibromas. Orcein stains
additionally identified fine oxytalan fibers which were
not stained by clusterin. Clusterin staining was observed
only on the outside of the elastin fibers, while the cores
of fibers and globules were unstained. 4/21
elastofibromas showed cellular nodules with a myxoid/
collagenous stroma. The round to oval cells showed
cytoplasmic staining with vimentin and clusterin; CD34
labelled mostly cell membranes. The cells lacked SMA
and S-100 expression. The central areas of the nodules
were devoid of elastic fibers, but the periphery contained
coarse fibers and globules. 9/11 patients, for whom
clinical data were available, reported trauma to the
scapular region. Conclusion: Many investigated ED
were associated with trauma, which supports a reactive/
degenerative etiology of ED. The abnormal large elastic
fibers in all ED were enveloped by clusterin. Clusterin
deposition may protect elastic fibers from degradation
and thus contribute indirectly to the tumor-like
presentation of ED
Clusterin Associates with Altered Elastic Fibers in Human Photoaged Skin and Prevents Elastin from Ultraviolet-Induced Aggregation in Vitro
Clusterin is a secreted glycoprotein with stress-induced expression in various diseased and aged tissues. It shares basic features with small heat shock proteins because it may stabilize proteins in a folding-competent state. Besides its presence in all human body fluids, clusterin associates with altered extracellular matrix proteins, such as β-amyloid in Alzheimer senile plaques in the brain. Because dermal connective tissue alterations occur because of aging and UV radiation, we explored the occurrence of clusterin in young, aged, and sun-exposed human skin. Immunohistochemical analysis showed that clusterin is constantly associated with altered elastic fibers in aged human skin. Elastotic material of sun-damaged skin (solar elastosis), in particular, revealed a strong staining for clusterin. Because of the striking co-localization of clusterin with abnormal elastic material, we investigated the interaction of clusterin with elastin in vitro. A chaperone assay was established in which elastin was denatured by UV irradiation in the absence or presence of clusterin. This assay demonstrated that clusterin exerted a chaperone-like activity and effectively inhibited UV-induced aggregation of elastin. The interaction of both proteins was further analyzed by electron microscopy, size exclusion chromatography, and mass spectrometry, in which clusterin was found in a stable complex with elastin after UV exposure