Association of hereditary elliptocytosis and Gilbert’s syndrome as the cause of biliary calculosis: Case report

Introduction. Biliary calculosis is rare in children. It occurs associated with different haemolytic and non-haemolytic disorders, which are sometimes also combined. Case Outline. A 15-year-old male was hospitalized due to biliary calculosis and non-conjugated hyper-bilirubinemia. A mild non-conjugated hyperbilirubinemia, without anaemia and other symptoms of liver dysfunction, was registered at age 8 years, and 7 years later cholelithiasis with transitory choledocholithiasis. The finding of ellyptocytes in blood smear, which was also verified in mother, normal haemoglobin count and the absence of diseases followed by secondary dysmorphic erythrocytes of this type, indicated a clinically milder (compensated) hereditary ellyptocytosis, while more than a double increase of non-conjugated serum bilirubin fracture after a three-day hypocaloric diet (400 kcal per day) showed the concurrent presence of Gilbert’s syndrome. In the laparascopically removed gallbladder a larger number of small pigmented calculi were disclosed. Conclusion. Gilbert’s syndrome is an essential precipitating factor of biliary calculosis in patients with chronic haemolytic condition. Thus, in all cases of biliary calculosis and non-conjugated hyperbilirubinemia with absent clinical and laboratory parameters of liver disorders and anaemia, except in compensated haemolytic disease and Gilbert’s syndrome as isolated disorders, a possibility of their association should be taken into consideration

Termički režim Negotinske krajine

Negotin Krajina geographically belongs to Mezian tablet that means it is widely open to the influences from the East but main air influences come from the West and North-West causes specific thermal characteristics. Meteorological station "Negotin" is the only one respectable station in the Krajina then we analyze results from various periods since 1901. It is important to emphasize that station "Negotin" doesn't have work continuity since the beginning of the century, thus for analyzing certain climate elements we have had to use method of analogy with station "Zaječar". A part of that, observations were not done by the same methodology so there is lack of some important indicators for this first period. To determine air thermo-regime of Negotin Krajina during 20 century, standard periods (1901-1930., 1931-1960. and 1961-1990) for analyzing climate elements suggested by WMO, were compared. Also, periods from 1925. to 1940. and from 1990. to 2000. were added to this analysis (according to data we had).Negotinska krajina fizički pripada Mezijskoj ploči, odnosno široko je okrenuta uticajima sa istoka, a glavne vazdušne mase dolaze sa zapada i severozapada uslovljavajući određene termičke specifičnosti. Metereološka stanica Negotin je jedina prvog reda u Krajini, tako da će se njeni rezultati analizirati i to za različite periode, počev od 1901. godine. Mora se napomenuti da stanica Negotin nema kontinuitet rada od početka veka, tako da se u nekim slučajevima morao koristiti metod analogije sa stanicom Zaječar. Osim toga ni osmatranja nisu vršena po istoj metodologiji, pa će tom prvom periodu nedostajati neki važni pokazatelji. Da bi se ustanovio termički režim vazduha u Negotinskoj krajini tokom XX veka biće upoređivani standardni periodi za obradu klimatskih elemenata, predloženih od WM0. Takođe, periodi od 1925. do 1940. i od 1990. do 2000. su dodati ovoj analizi (prema podacima kojima se raspolagalo)

Termički režim Negotinske krajine

Negotin Krajina geographically belongs to Mezian tablet that means it is widely open to the influences from the East but main air influences come from the West and North-West causes specific thermal characteristics. Meteorological station "Negotin" is the only one respectable station in the Krajina then we analyze results from various periods since 1901. It is important to emphasize that station "Negotin" doesn't have work continuity since the beginning of the century, thus for analyzing certain climate elements we have had to use method of analogy with station "Zaječar". A part of that, observations were not done by the same methodology so there is lack of some important indicators for this first period. To determine air thermo-regime of Negotin Krajina during 20 century, standard periods (1901-1930., 1931-1960. and 1961-1990) for analyzing climate elements suggested by WMO, were compared. Also, periods from 1925. to 1940. and from 1990. to 2000. were added to this analysis (according to data we had).Negotinska krajina fizički pripada Mezijskoj ploči, odnosno široko je okrenuta uticajima sa istoka, a glavne vazdušne mase dolaze sa zapada i severozapada uslovljavajući određene termičke specifičnosti. Metereološka stanica Negotin je jedina prvog reda u Krajini, tako da će se njeni rezultati analizirati i to za različite periode, počev od 1901. godine. Mora se napomenuti da stanica Negotin nema kontinuitet rada od početka veka, tako da se u nekim slučajevima morao koristiti metod analogije sa stanicom Zaječar. Osim toga ni osmatranja nisu vršena po istoj metodologiji, pa će tom prvom periodu nedostajati neki važni pokazatelji. Da bi se ustanovio termički režim vazduha u Negotinskoj krajini tokom XX veka biće upoređivani standardni periodi za obradu klimatskih elemenata, predloženih od WM0. Takođe, periodi od 1925. do 1940. i od 1990. do 2000. su dodati ovoj analizi (prema podacima kojima se raspolagalo)

Leaf total transcriptome analysis enriches insight into maize abiotic stress signaling network

Abiotic stresses cause great losses in crop production worldwide. Due to climatic changes temperatures higher than the average are frequently observed in temperate areas in the course of maize flowering and grain filling period, displaying detrimental effect on maize yield. In order to overcome this problem development of drought tolerant maize lines, but also cold tolerant maize lines that would allow earlier sowing and hence avoidance of extremely high temperatures during sensitive developmental phases in maize, became important goal of maize breeding programs in temperate areas. A set of 46 maize inbred lines was chosen from three main heterotic groups (BSSS, Lancaster, Iowa dent) important for commercial breeding programs. Plants were grown under the optimal conditions in the greenhouse and sampled upon entering V5 growth stage. A total RNA isolated from leaves of three plants of each inbred line was used for DNA library preparation by Illumina TruSeq Stranded RNA LT kit. Pair-end sequencing was performed on MiSeq Illumina sequencer using MiSeq Reagent kit v2 (2x150bp). For data manipulation and analysis a custom made bioinformatics pipeline was used. Differential gene expression analysis revealed a minimum of 21 genes that were strongly statistically supported for differential expression between the inbred lines and also annotated as involved in abiotic stress responses in other plant species. We tested 10 of these genes in maize by using qPCR on the subset of 8 inbred lines grown under optimal, cold and drought conditions. Real-time PCR results revealed significant change in the expression level of tested genes under abiotic stresses, supporting bioinformatics prediction. Verified genes were then submitted to free online gene networking tools and after determining relationships between predicted networks and inbred line phenotypes, possible novel gene candidates for enriching maize abiotic stress signaling network resolution were proposed

Thermal regime of Negotinska Krajina

Negotin Krajina geographically belongs to Mezian tablet that means it is widely open to the influences from the East but main air influences come from the West and North-West causes specific thermal characteristics. Meteorological station "Negotin" is the only one respectable station in the Krajina then we analyze results from various periods since 1901. It is important to emphasize that station "Negotin" doesn't have work continuity since the beginning of the century, thus for analyzing certain climate elements we have had to use method of analogy with station "Zaječar". A part of that, observations were not done by the same methodology so there is lack of some important indicators for this first period. To determine air thermo-regime of Negotin Krajina during 20 century, standard periods (1901-1930., 1931-1960. and 1961-1990) for analyzing climate elements suggested by WMO, were compared. Also, periods from 1925. to 1940. and from 1990. to 2000. were added to this analysis (according to data we had)