Synergistic Antibacterial Effect between Silibinin and Antibiotics in Oral Bacteria

Silibinin is a composition of the silymarin group as a hepatoprotective agent, and it exhibits various biological activities, including antibacterial activity. In this study, the antibacterial activities of silibinin were investigated in combination with two antimicrobial agents against oral bacteria. Silibinin was determined with MIC and MBC values ranging from 0.1 to 3.2 and 0.2 to 6.4 μg/mL, ampicillin from 0.125 to 64 and 0.5 to 64 μg/mL, gentamicin from 2 to 256 and 4 to 512 μg/mL, respectively. The ranges of MIC50 and MIC90 were 0.025–0.8 μg/mL and 0.1–3.2 μg/mL, respectively. The antibacterial activities of silibinin against oral bacteria were assessed using the checkerboard and time-kill methods to evaluate the synergistic effects of treatment with ampicillin or gentamicin. The results were evaluated showing that the combination effects of silibinin with antibiotics were synergistic (FIC index <0.5) against all tested oral bacteria. Furthermore, a time-kill study showed that the growth of the tested bacteria was completely attenuated after 2–6 h of treatment with the MBC of silibinin, regardless of whether it was administered alone or with ampicillin or gentamicin. These results suggest that silibinin combined with other antibiotics may be microbiologically beneficial and not antagonistic

Differences in Oral Health and Generalized Anxiety Disorder According to Secondhand Smoke Exposure in Public Places

Background: This study aims to provide basic data for minimizing exposure to secondhand smoke (SHS) by confirming the relationship between exposure to SHS and generalized anxiety disorder (GAD). Methods: Using the third year data of the eighth National Health and Nutrition Examination Survey, 3874 people were selected as subjects. Complex sampling analysis was applied to all analyses, and 307 people were exposed to the SHS group (SHSG) and 3567 people were not exposed to the SHS group (NSHSG). Complex sample linear regression analysis was conducted to confirm the effect of exposure to SHS on oral health and GAD. Results: Among oral-health-related factors, exposure to SHS in Korean adults was related to the presence of implants. Additionally, exposure to SHS had a significant effect on GAD despite adjusting for sociodemographic characteristics and oral-health-related factors (p < 0.05). Conclusions: In this study, the relationship between passive smoking and GAD was confirmed. Therefore, to reduce GAD, oral health management is necessary and exposure to SHS should be minimized

Effects of Muscular Strength Training on Oral Health and Quality of Life: Using Korean Panel Survey Data, a Cross-Sectional Study

Objectives: The purpose of this study is to confirm the importance of muscular strength exercise by confirming the relationship between strength exercise, oral health, and quality of life. Methods: Using the 2019 and 2021 of the Korean National Health and Nutrition Examination Survey (KNHANES), 6535 people were selected as subjects. Complex sampling analysis was applied to all analyses; 2267 people were in the muscular strength training group (MSG), and 5841 people were in the non-muscular strength training group (NMSG). A multi-sample linear regression analysis was conducted to confirm the effect of muscular strength training on oral health and quality of life. Results: As a result of confirming the effect of muscular strength training on oral health status, problems with chewing decreased by 0.105, and problems with speaking decreased by 0.028 with MSG compared to NMSG. In addition, compared to NMSG, it was confirmed that MSG reduced chewing discomfort by 0.047, while self-perceived oral health improved by 0.0123. Finally, as a result of confirming the effect of muscular strength training on oral health and quality of life in Korean adults, there was a significant effect on quality of life despite adjusting for sociodemographic characteristics and oral-health-related factors (p 0.05). Conclusions: In this study, the relationship between muscular strength training and quality of life was confirmed. Therefore, efforts should be made to make oral health management and muscular strength training a part of life in relation to quality of life

Synergistic antibacterial effect between silibinin and antibiotics in oral bacteria,” 6 Evidence-Based Complementary and Alternative Medicine

Silibinin is a composition of the silymarin group as a hepatoprotective agent, and it exhibits various biological activities, including antibacterial activity. In this study, the antibacterial activities of silibinin were investigated in combination with two antimicrobial agents against oral bacteria. Silibinin was determined with MIC and MBC values ranging from 0.1 to 3.2 and 0.2 to 6.4 µg/mL, ampicillin from 0.125 to 64 and 0.5 to 64 µg/mL, gentamicin from 2 to 256 and 4 to 512 µg/mL, respectively. The ranges of MIC 50 and MIC 90 were 0.025-0.8 µg/mL and 0.1-3.2 µg/mL, respectively. The antibacterial activities of silibinin against oral bacteria were assessed using the checkerboard and time-kill methods to evaluate the synergistic effects of treatment with ampicillin or gentamicin. The results were evaluated showing that the combination effects of silibinin with antibiotics were synergistic (FIC index &lt; 0.5) against all tested oral bacteria. Furthermore, a time-kill study showed that the growth of the tested bacteria was completely attenuated after 2-6 h of treatment with the MBC of silibinin, regardless of whether it was administered alone or with ampicillin or gentamicin. These results suggest that silibinin combined with other antibiotics may be microbiologically beneficial and not antagonistic