Effect of Triclosan and Silver Nanoparticles on DNA Damage Investigated with DNA-Based Biosensor

Triclosan (TCS) is a broad-spectrum antimicrobial agent widely used in personal care, healthcare, and clinical practice. One of the most important aspects of toxicological profiling of compounds is their interaction with DNA. In human cells, TCS causes a significant reduction in DNA methylation. The involvement of TCS in chromosomal aberrations, DNA damage, and strand breaks, as well as DNA damage from TCS degradation products, was reported. AgNPs share similarities with TCS in terms of antimicrobial properties, enter the body after exposure, and are used even together with TCS in oral care products. Therefore, their mutual effect on the DNA is of interest. In this study, the electrochemical behavior of TCS on a glassy carbon electrode (GCE) and the biosensor with salmon sperm dsDNA (DNA/GCE), DNA damage by TCS present in phosphate buffer solution pH 7.4 and an additional effect of the immobilized AgNP layer on such DNA damage have been investigated. Two different sizes of AgNPs (about 15 and 37 nm) were tested. Using square-wave voltammetric signals of nucleobases, the portion of survived DNA was 64% in the presence of 15 nm AgNPs compared to 55% in its absence. The protective effect of AgNPs on DNA against TCS-induced DNA damage was found

Ethanol and NaCl-Induced Gold Nanoparticle Aggregation Toxicity toward DNA Investigated with a DNA/GCE Biosensor

Engineered nanomaterials are becoming increasingly common in commercial and consumer products and pose a serious toxicological threat. Exposure of human organisms to nanomaterials can occur by inhalation, oral intake, or dermal transport. Together with the consumption of alcohol in the physiological environment of the body containing NaCl, this has raised concerns about the potentially harmful effects of ingested nanomaterials on human health. Although gold nanoparticles (AuNPs) exhibit great potential for various biomedical applications, there is some inconsistency in the case of the unambiguous genotoxicity of AuNPs due to differences in their shape, size, solubility, and exposure time. A DNA/GCE (DNA/glassy carbon electrode) biosensor was used to study ethanol (EtOH) and NaCl-induced gold nanoparticle aggregation genotoxicity under UV light in this study. The genotoxic effect of dispersed and aggregated negatively charged gold nanoparticles AuNP1 (8 nm) and AuNP2 (30 nm) toward salmon sperm double-stranded dsDNA was monitored by cyclic and square-wave voltammetry (CV, SWV). Electrochemical impedance spectroscopy (EIS) was used for a surface study of the biosensor. The aggregation of AuNPs was monitored by UV-vis spectroscopy. AuNP1 aggregates formed by 30% v/v EtOH and 0.15 mol·L−1 NaCl caused the greatest damage to the biosensor DNA layer

Effects of the Time Period Length on the Determination of Long-Term Mean Annual Discharge

Currently, the 40-year-long reference period 1961–2000 is used in Slovakia for systematic assessment of the hydrological regime. The World Meteorological Organization recommends unifying the climatic normal 1991–2020 with a reference period for hydrological characteristics for the purpose of climate change research. We have analyzed changes in the long-term mean annual discharges at 113 water-gauging stations over different time periods. Considering the history of the reference periods used in Slovakia, we have focused on the analysis of the 10, 20, 30, 40, and 50-year-long moving averages of the long-term mean annual discharges in the period 1961–2020 in comparison with the 1961–2000 reference period and the whole selected and analyzed period. The deviations of the annual flow rates for the area of Slovakia and river sub-basins have been analyzed. The results indicate that the new reference period to be used in Slovakia should include the time period after the year 2000. To determine its precise length, a more detailed analysis is needed

Changes in Selected Low-Flow Characteristics in the 2001–2015 Period Compared to the 1961–2000 Reference Period in Slovakia

This research is focused on the assessment of drought on surface watercourses in Slovakia. Low-flow characteristics and their changes in the 2001–2015 period in comparison with the 1961–2000 reference period were evaluated at selected representative water-gauging stations. Two different methods were used to calculate the flow duration curves (FDCs): the standard method, based on mean daily discharge data series for the whole evaluated period in descending order, and the alternative method, based on first calculating the values of FDCs for each year of the assessed period and then averaging the corresponding percentile values. The changes were evaluated for selected percentiles of the FDCs (330-, 355-, and 364-day discharge). The number of days with the mean daily flow below the set limits and the seasonality of their occurrence were assessed. The results show significant changes in cases of both methods in the compared time periods, while differences in individual regions of Slovakia were also found. The weakness of the standard method is in allowing the values of the smallest quantiles to be influenced by a small number of long-lasting drought episodes. The alternative method eliminates the aforementioned shortcoming and could be used to determine the ecological flows in Slovakia