The effects of parent effectiveness training on parents\u27 personal and child rearing values

There is growing evidence of a need for training parents to be more effective in child-rearing practices regarding values acquisition and clarification. The primary objective of this study was to investigate the effectiveness of Parent Effectiveness Training, a parent education program, in changing the personal and child-rearing values of the participating parents

Tennessee practitioner perceptions of the construct, content, and utility of Exemplary Practices in Alternative Education: Indicators of Quality Programming for use as an evaluation instrument

This dissertation explored Tennessee practitioner perceptions of the construct, content and utility of Exemplary Practices in Alternative Education: Indicators of Quality Programming (Exemplary Practices) for use as an evaluation instrument (National Alternative Education Association, 2009). The general purposes of this study were to (1) examine the legitimacy of the ten constructs (i.e., standards) and corresponding content (i.e., indicators of success) of best practice as presented in the Exemplary Practices and (2) investigate the utility of the Exemplary Practices when transformed into an evaluation instrument for alternative schools and programs. The study entailed a two-phased sequential, mixed-model research design (Cameron, 2009). Phase One involved a concurrent embedded strategy (Creswell, 2009) to obtain quantitative and qualitative data related to the constructs and content found in the Exemplary Practices. With the exception of four indicators, findings provide evidence of construct and content validity as perceived by Tennessee practitioners. Phase Two involved a sequential, explanatory research strategy (Creswell, 2009) aimed at collecting data related to the utility of the Exemplary Practices when transformed into an evaluation instrument. Findings indicate that the majority of constructs and content were not observable during utility testing. Additionally, findings point to the need for enhancements to the instrument. During utility testing, simple observations were not enough to fully ascertain whether or not the alternative school or program was implementing the Exemplary Practices with fidelity. Research participants overwhelming noted that the evaluation instrument should incorporate evidence categories for observations, interviews and artifacts. Following Phase One and Phase Two of the study, the researcher developed an evaluation instrument for designing, delivering, evaluating and improving alternative education programming. The instrument was constructed from the Exemplary Practices but adapted based upon practitioner perceptions of construct and content validity, as well as overall utility. The culminating evaluation instrument is presented as a product of the research study

SENP1 participates in the dynamic regulation of Elk-1 SUMOylation

The modification of proteins with SUMO (small ubiquitin-related modifier) plays an important role in determining their functional properties. Importantly though, SUMOylation is a highly dynamic process enabling transient responses to be elicited. This dynamism is controlled by two competing conjugating and deconjugating activities. The latter activity is mediated by the SENP [SUMO1/sentrin/SMT3 (suppressor of mif two 3 homologue 1)-specific peptidase] family of SUMO-specific proteases. The transcription factor Elk-1 [ETS (E twenty-six)-like 1] undergoes rapid de-SUMOylation following cellular stimulation with growth factors, and this contributes to its conversion from a SUMO-dependent repressor into a potent transcriptional activator. In the present study we demonstrate an important role for SENP1 in the de-SUMOylation of Elk-1, and therefore an integral role in determining the Elk-1-dependent transcriptional programme. Among the SENPs, Elk-1 preferentially forms a complex with SENP1. This preferential binding is reflected by the higher efficiency of SENP1 in promoting Elk-1 transactivation. Moreover, depletion of SENP1 causes a reciprocal effect and reduces the transactivation properties of Elk-1. Partial redundancy of function with SENP2 is revealed by combinatorial knockdown studies. Importantly, depletion of SENP1 also reduces the activation of the Elk-1 target gene c-FOS. Taken together, these results therefore reveal an important role for SENP1 in the regulation of Elk-1-mediated gene expression in response to mitogenic signalling cues

Vacuolar organization in the nodule parenchyma is important for the functioning of pea root nodules

Different models have been proposed to explain the operation of oxygen diffusion barrier in root nodules of leguminous plants. This barrier participates in protection of oxygen-sensitive nitrogenase, the key enzyme in nitrogen fixation, from inactivation. Details concerning structural and biochemical properties of the barrier are still lacking. Here, the properties of pea root nodule cortical cells were examined under normal conditions and after shoot removal. Microscopic observations, including neutral red staining and epifluorescence investigations, showed that the inner and outer nodule parenchyma cells exhibit different patterns of the central vacuole development. In opposition to the inner part, the outer parenchyma cells exhibited vacuolar shrinkage and formed cell wall infoldings. Shoot removal induced vacuolar shrinkage and formation of infoldings in the inner parenchyma and uninfected cells of the symbiotic tissue, as well. It is postulated that cells which possess shrinking vacuoles are sensitive to the external osmotic pressure. The cells can give an additional resistance to oxygen diffusion by release of water to the intercellular spaces

From graduate employability to employment: policy and practice in UK higher education

© 2017 Brian Towers (BRITOW) and John Wiley & Sons Ltd. The purpose of this paper is to enrich the current conceptualization of graduate employability and employment through the lens of policy, academia and practice in UK higher education. We examine the UK policy context that is shaping graduate employability and employment debates before enriching this conceptualization through a discussion of key themes in the academic literature. We then undertake a comparative study across a sample of 35 higher education institutions in the UK to identify and discuss key employability practice areas shaping their graduate employability and employment provision. We do so by drawing on institutional employability data that is already available in the public domain. In mapping key themes in the literature against institutional employability practice, we conclude that the employer-university interaction theme in the literature is not sufficiently addressed in UK higher education practice. Drawing on the evidence from our comparative study, we provide a discussion on four directions worthy of further exploration by universities as they shape their institutional responses to the lack of employer-university interaction

UCT943, a next generation Plasmodium falciparum PI4K inhibitor preclinical candidate for the treatment of malaria

The 2-aminopyridine MMV048 was the first drug candidate inhibiting; Plasmodium; phosphatidylinositol 4-kinase (PI4K), a novel drug target for malaria, to enter clinical development. In an effort to identify the next generation of PI4K inhibitors, the series was optimized to improve properties such as solubility and antiplasmodial potency across the parasite life cycle, leading to the 2-aminopyrazine UCT943. The compound displayed higher asexual blood stage, transmission-blocking, and liver stage activities than MMV048 and was more potent against resistant; Plasmodium falciparum; and; Plasmodium vivax; clinical isolates. Excellent; in vitro; antiplasmodial activity translated into high efficacy in; Plasmodium berghei; and humanized; P. falciparum; NOD-; scid IL-2R; γ; null; mouse models. The high passive permeability and high aqueous solubility of UCT943, combined with low to moderate; in vivo; intrinsic clearance, resulted in sustained exposure and high bioavailability in preclinical species. In addition, the predicted human dose for a curative single administration using monkey and dog pharmacokinetics was low, ranging from 50 to 80 mg. As a next-generation; Plasmodium; PI4K inhibitor, UCT943, based on the combined preclinical data, has the potential to form part of a single-exposure radical cure and prophylaxis (SERCaP) to treat, prevent, and block the transmission of malaria

A blood atlas of COVID-19 defines hallmarks of disease severity and specificity.

Treatment of severe COVID-19 is currently limited by clinical heterogeneity and incomplete description of specific immune biomarkers. We present here a comprehensive multi-omic blood atlas for patients with varying COVID-19 severity in an integrated comparison with influenza and sepsis patients versus healthy volunteers. We identify immune signatures and correlates of host response. Hallmarks of disease severity involved cells, their inflammatory mediators and networks, including progenitor cells and specific myeloid and lymphocyte subsets, features of the immune repertoire, acute phase response, metabolism, and coagulation. Persisting immune activation involving AP-1/p38MAPK was a specific feature of COVID-19. The plasma proteome enabled sub-phenotyping into patient clusters, predictive of severity and outcome. Systems-based integrative analyses including tensor and matrix decomposition of all modalities revealed feature groupings linked with severity and specificity compared to influenza and sepsis. Our approach and blood atlas will support future drug development, clinical trial design, and personalized medicine approaches for COVID-19

The effects of parent effectiveness training on parents\u27 personal and child rearing values

There is growing evidence of a need for training parents to be more effective in child-rearing practices regarding values acquisition and clarification. The primary objective of this study was to investigate the effectiveness of Parent Effectiveness Training, a parent education program, in changing the personal and child-rearing values of the participating parents

Regulation of transcription factor sumoylation

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