Optovibrometry: tracking changes in the surface tension and viscosity of multicomponent droplets in real-time

An instrument was developed for measuring real time changes in the surface tension and viscosity of multicomponent droplets of miscible liquids and other soft materials. Droplets containing glycerol and water were supported on superamphiphobic surfaces and vibrated by applying a short mechanical impulse. Laser light was refracted through the droplets and allowed to fall on the surface of a photodiode. Time dependent variations in the intensity measured by the photodiode during vibration were used to monitor the decay of the droplet oscillations. The frequencies and spectral widths of the droplet vibrational resonances were then obtained from Fourier transforms of these time dependent intensity signals. A recently developed model of viscoelastic droplet vibration was used along with these values and measurements of the drop dimensions to extract the surface tension and viscosity of the drops as they evaporated. Collection of data was automated and values of frequency, spectral width, drop size, surface tension and viscosity were obtained with a time resolution of three seconds over a period of thirty minutes. The values of surface tension and viscosity obtained were shown to be in good agreement with literature values obtained from bulk glycerol/water solutions; thus validating the technique for wider application to other multicomponent liquids and soft matter systems

Shear banding in drying films of colloidal nanoparticles

Drying suspensions of colloidal nanoparticles exhibit a variety of interesting strain release mechanisms during film formation. These result in the selection of characteristic length scales during failure processes such as cracking and subsequent delamination. A wide range of materials (e.g., bulk metallic glasses) release strain through plastic deformations which occur in a narrow band of material known as a shear band. Here we show that drying colloidal films also exhibit shear banding. Bands are observed to form a small distance behind the drying front and then to propagate rapidly at ∼45� to the direction of drying. It is shown that the spacing of the bands depends on salt concentration and the evaporation rate of the colloidal suspension. These combined observations suggest that there is a critical shear rate (related to the film yield stress) which controls the ratio of bandwidth to band spacing. Local deformations were measured in the early stages of drying using fluorescent tracer particles. The measurements were used to show that the existence of shear bands is linked to the compaction of particles perpendicular to the drying front. The spacing of shear bands was also found to be strongly correlated with the characteristic length scale of the compaction process. These combined studies elucidate the role of plastic deformation during pattern formation in drying films of colloidal nanoparticles

Challenging empowerment: AIDS-affected southern African children and the need for a multi-level relational approach

Critics of empowerment have highlighted the concept's mutability, focus on individual transformation, one-dimensionality and challenges of operationalisation. Relating these critiques to children's empowerment raises new challenges. Drawing on scholarship on children's subjecthood and exercise of power, alongside empirical research with children affected by AIDS, I argue that empowerment envisaged as individual self-transformation and increased capacity to act independently offers little basis for progressive change. Rather it is essential to adopt a relational approach that recognises the need to transform power relationships at multiple levels. This analysis has implications for our wider understanding of empowerment in the 21st century. © The Author(s) 2013.This research was funded by DFID

Tracing Nitrogen in Volcanic and Geothermal Volatiles from the Nicaraguan Volcanic Front

We report new chemical and isotopic data from 26 volcanic and geothermal gases, vapor condensates, and thermal water samples, collected along the Nicaraguan volcanic front. The samples were analyzed for chemical abundances and stable isotope compositions, with a focus on nitrogen abundances and isotope ratios. These data are used to evaluate samples for volatile contributions from magma, air, air-saturated water, and the crust. Samples devoid of crustal contamination (based upon He isotope composition) but slightly contaminated by air or air-saturated water are corrected using N2/Ar ratios in order to obtain primary magmatic values, composed of contributions from upper mantle and subducted hemipelagic sediment on the down-going plate. Using a mantle endmember with d15N= 5&and N2/He = 100 and a subducted sediment component with d15N=+7& and N2/He = 10,500, the average sediment contribution to Nicaraguan volcanic and geothermal gases was determined to be 71%. Most of the gases were dominated by sediment-derived nitrogen, but gas from Volca´n Mombacho, the southernmost sampling location, had a mantle signature (46% from subducted sediment, or 54% from the mantle) and an affinity with mantle-dominated gases discharging from Costa Rica localities to the south. High CO2/N2 exc. ratios (N2 exc. is the N2 abundance corrected for contributions from air) in the south are similar to those in Costa Rica, and reflect the predominant mantle wedge input, whereas low ratios in the north indicate contribution by altered oceanic crust and/or preferential release of nitrogen over carbon from the subducting slab. Sediment-derived nitrogen fluxes at the Nicaraguan volcanic front, estimated by three methods, are 7.8 · 108 mol N/a from 3He flux, 6.9 · 108 mol/a from SO2 flux, and 2.1 · 108 and 1.3 · 109 mol/a from CO2 fluxes calculated from 3He and SO2, respectively. These flux results are higher than previous estimates for Central America, reflecting the high sediment-derived volatile contribution and the high nitrogen content of geothermal and volcanic gases in Nicaragua. The fluxes are also similar to but higher than estimated hemipelagic nitrogen inputs at the trench, suggesting addition of N from altered oceanic basement is needed to satisfy these flux estimates. The similarity of the calculated input of N via the trench to our calculated outputs suggests that little or none of the subducted nitrogen is being recycled into the deeper mantle, and that it is, instead, returned to the surface via arc volcanism

PCA-based lung motion model

Organ motion induced by respiration may cause clinically significant targeting errors and greatly degrade the effectiveness of conformal radiotherapy. It is therefore crucial to be able to model respiratory motion accurately. A recently proposed lung motion model based on principal component analysis (PCA) has been shown to be promising on a few patients. However, there is still a need to understand the underlying reason why it works. In this paper, we present a much deeper and detailed analysis of the PCA-based lung motion model. We provide the theoretical justification of the effectiveness of PCA in modeling lung motion. We also prove that under certain conditions, the PCA motion model is equivalent to 5D motion model, which is based on physiology and anatomy of the lung. The modeling power of PCA model was tested on clinical data and the average 3D error was found to be below 1 mm.Comment: 4 pages, 1 figure. submitted to International Conference on the use of Computers in Radiation Therapy 201

Mechanical vibrations of pendant liquid droplets

A simple optical deflection technique was used to monitor the vibrations of microlitre pendant droplets of deuterium oxide, formamide, and 1,1,2,2-tetrabromoethane. Droplets of different volumes of each liquid were suspended from the end of a microlitre pipette and vibrated using a small puff of nitrogen gas. A laser was passed through the droplets and the scattered light was collected using a photodiode. Vibration of the droplets resulted in the motion of the scattered beam and time-dependent intensity variations were recorded using the photodiode. These time- dependent variations were Fourier transformed and the frequencies and widths of the mechanical droplet resonances were extracted. A simple model of vibrations in pendant/sessile drops was used to relate these parameters to the surface tension, density and viscosity of the liquid droplets. The surface tension values obtained from this method were found to be in good agreement with results obtained using the standard pendant drop technique. Damping of capillary waves on pendant drops was shown to be similar to that observed for deep liquid baths and the kinematic viscosities obtained were in agreement with literature values for all three liquids studied

Alginate inhibits iron absorption from ferrous gluconate in a randomized controlled trial and reduces iron uptake into Caco-2 cells

Previous in vitro results indicated that alginate beads might be a useful vehicle for food iron fortification. A human study was undertaken to test the hypothesis that alginate enhances iron absorption. A randomised, single blinded, cross-over trial was carried out in which iron absorption was measured from serum iron appearance after a test meal. Overnight-fasted volunteers (n=15) were given a test meal of 200g cola-flavoured jelly plus 21 mg iron as ferrous gluconate, either in alginate beads mixed into the jelly or in a capsule. Iron absorption was lower from the alginate beads than from ferrous gluconate (8.5% and 12.6% respectively, p=0.003). Sub-group B (n=9) consumed the test meals together with 600 mg calcium to determine whether alginate modified the inhibitory effect of calcium. Calcium reduced iron absorption from ferrous gluconate by 51%, from 11.5% to 5.6% (p=0.014), and from alginate beads by 37%, from 8.3% to 5.2% (p=0.009). In vitro studies using Caco-2 cells were designed to explore the reasons for the difference between the previous in vitro findings and the human study; confirmed the inhibitory effect of alginate. Beads similar to those used in the human study were subjected to simulated gastrointestinal digestion, with and without cola jelly, and the digestate applied to Caco-2 cells. Both alginate and cola jelly significantly reduced iron uptake into the cells, by 34% (p=0.009) and 35% (p=0.003) respectively. The combination of cola jelly and calcium produced a very low ferritin response, 16.5% (p<0.001) of that observed with ferrous gluconate alone. The results of these studies demonstrate that alginate beads are not a useful delivery system for soluble salts of iron for the purpose of food fortification

Accessible biometrics: a frustrated total internal reflection approach to imaging fingerprints

Fingerprints are widely used as a means of identifying persons of interest because of the highly individual nature of the spatial distribution and types of features (or minuta) found on the surface of a finger. This individuality has led to their wide application in the comparison of fingerprints found at crime scenes with those taken from known offenders and suspects in custody. However, despite recent advances in machine vision technology and image processing techniques, fingerprint evidence is still widely being collected using outdated practices involving ink and paper – a process that can be both time consuming and expensive. Reduction of forensic service budgets increasingly requires that evidence be gathered and processed more rapidly and efficiently. However, many of the existing digital fingerprint acquisition devices have proven too expensive to roll out on a large scale. As a result new, low-cost imaging technologies are required to increase the quality and throughput of the processing of fingerprint evidence. Here we describe an inexpensive approach to digital fingerprint acquisition that is based upon frustrated total internal reflection imaging. The quality and resolution of the images produced are shown to be as good as those currently acquired using ink and paper based methods. The same imaging technique is also shown to be capable of imaging powdered fingerprints that have been lifted from a crime scene using adhesive tape or gel lifters

Stops making sense: translational trade-offs and stop codon reassignment

Background Efficient gene expression involves a trade-off between (i) premature termination of protein synthesis; and (ii) readthrough, where the ribosome fails to dissociate at the terminal stop. Sense codons that are similar in sequence to stop codons are more susceptible to nonsense mutation, and are also likely to be more susceptible to transcriptional or translational errors causing premature termination. We therefore expect this trade-off to be influenced by the number of stop codons in the genetic code. Although genetic codes are highly constrained, stop codon number appears to be their most volatile feature. Results In the human genome, codons readily mutable to stops are underrepresented in coding sequences. We construct a simple mathematical model based on the relative likelihoods of premature termination and readthrough. When readthrough occurs, the resultant protein has a tail of amino acid residues incorrectly added to the C-terminus. Our results depend strongly on the number of stop codons in the genetic code. When the code has more stop codons, premature termination is relatively more likely, particularly for longer genes. When the code has fewer stop codons, the length of the tail added by readthrough will, on average, be longer, and thus more deleterious. Comparative analysis of taxa with a range of stop codon numbers suggests that genomes whose code includes more stop codons have shorter coding sequences. Conclusions We suggest that the differing trade-offs presented by alternative genetic codes may result in differences in genome structure. More speculatively, multiple stop codons may mitigate readthrough, counteracting the disadvantage of a higher rate of nonsense mutation. This could help explain the puzzling overrepresentation of stop codons in the canonical genetic code and most variants

Dual optical force plate for time resolved measurement of forces and pressure distributions beneath shoes and feet

Frustrated total internal reflection (FTIR) imaging was used to perform remote optical measurements of the forces/pressures exerted beneath shoes and feet during a number of different training activities including countermovement jumps, jogging and drop jumps. A single camera was used to simultaneously image two acrylic, FTIR waveguide imaging elements from below, at frame rates up to 200 frames per second. The images obtained using the camera were converted into pressure/force maps using a previously developed theory which combines the mechanics of contact of soft objects and the scattering of evanescent waves. The forces obtained from the optical measurements were shown to be in good agreement with measurements obtained from load cells placed beneath the FTIR imaging elements. The ability to produce accurate spatial maps of the force/pressure distribution beneath soft contacting objects such as feet and shoe outsoles at high frame rates has numerous potential applications in sports sciences and medicine