Use of a lambda gt11 expression library to localize a neutralizing antibody-binding site in glycoprotein E2 of Sindbis virus

The Sindbis virus envelope contains two species of integral membrane glycoproteins, E1 and E2. These proteins form heterodimers, and three dimeric units assemble to form spikes incorporated into the viral surface which play an important role in the specific attachment of Sindbis virus to host cells. To map the neutralization epitopes on the surface of the virus, we constructed a lambda gt11 expression library with cDNA inserts 100 to 300 nucleotides long obtained from randomly primed synthesis on Sindbis virus genomic RNA. This library was screened with five different neutralizing monoclonal antibodies (MAbs) specific for E2 (MAbs 50, 51, 49, 18, and 23) and with one neutralizing MAb specific for E1 (MAb 33). When 10(6) lambda gt11 plaques were screened with each antibody, four positive clones that reacted with E2-specific MAb 23 were found. These four clones contained overlapping inserts from glycoprotein E2; the domain from residues 173 to 220 of glycoprotein E2 was present in all inserts, and we concluded that this region contains the neutralization epitope recognized by the antibody. No clones that reacted with the other antibodies examined were found, and we concluded that these antibodies probably recognize conformational epitopes not present in the lambda gt11 library. We suggest that the E2 domain from residues 173 to 220 is a major antigenic determinant of Sindbis virus and that this domain is important for virus attachment to cells

The Effects of Neoliberal "Reforms" on the Post-Crisis Korean Economy

In December 1997 the IMF offered Korea loans to help alleviate its financial crisis. These loans were accompanied by what the IMF called “extreme structural conditionality.” Korea was required to replace its traditional East Asian economic system with a neoliberal model. We review economic performance in the neoliberal era. Growth has slowed, poverty and inequality have risen, and investment spending has stagnated, while foreign ownership of Korean firms and banks has skyrocketed. We argue that foreign investment has not helped Korea. For example, by leading a shift from corporate to consumer lending, foreign control of Korea’s financial markets has constrained capital accumulation and helped create an excessively indebted household sector, while making it harder for the government to adopt progressive economic policies. We conclude that the eight year experiment with radical neoliberal restructuring has turned out well for foreign capital and wealthy Koreans, but has been a failure for the majority of Korea’s people.

Was the IMF's Imposition of Economic Regime Change Justified? A Critique of the IMF's Economic and Political Role in Korea During and After the Crisis

As late as October 1997 the IMF declared that the Korean economy was experiencing a temporary liquidity squeeze, not a solvency problem. Yet in December 1997 Deputy Managing Director Stanley Fischer declared that Korea suffered from a systemic “breakdown of economic relations” so complete that only radical economic restructuring could restore prosperity. The IMF attached what it called “extreme structural conditionality” to its loan agreements with Korea, demanding a complete and rapid transition from Korea’s traditional East Asian economic model to a globally integrated neoliberal model. We subject the IMF’s assertion that the allocative efficiency of the Korean economy had collapsed by 1997 to a number of empirical tests, including time series and cross-section analyses of capital productivity and corporate profitability, and firm and industry level econometric tests of the proposition that investment spending was excessive and misallocated in the pre-crisis period. This evidence does not support the IMF’s systemic breakdown claim. We conclude that the IMF’s imposition of “extreme structural conditionality” on Korea is best understood as an illegitimate and antidemocratic exercise of power designed to meet the needs of the IMF’s key constituents rather than those of the majority of Korea’s people.

Economic Performance in Post-Crisis Korea: A Critical Perspective on Neo-Liberal Restructuring

This paper evaluates the neoliberal economic restructuring process implemented in Korea following the 1997 Asian financial crisis. We first argue that the austerity macroeconomic policy of late 1997 and early 1998 was the main cause of the economic collapse in 1998, and that the decision of the IMF and President Kim Dae Jung to impose a radical neoliberal transformation of financial markets and large industrial firms in the depressed conditions of 1998, though defensible on political grounds, made the failure of these reforms virtually inevitable. A detailed analysis of the macro economy, labor markets, financial markets, and nonfinancial firms in Korea in the past three and one-half years shows that neoliberal restructuring has created a vicious cycle in which a perpetually weak financial sector fails to provide the capital needed for real sector growth, investment and financial robustness, while real sector financial fragility continuously weakens financial firms. Neoliberal policies may have pushed Korea onto a low-investment, low-growth, development path, one with rising insecurity and inequality. Meanwhile, the removal of virtually all restrictions on cross-border capital flows has led to a dramatic increase in the influence of foreign capital in Korea's economy. The paper concludes by arguing that Korea should reject radical neoliberal restructuring and instead adopt reforms designed to democratize and modernize its traditional state-guided growth model.Globalization; Korean crisis; neoliberalism; economic restructuring; Korean economic model

Learning scalable and transferable multi-robot/machine sequential assignment planning via graph embedding

Can the success of reinforcement learning methods for simple combinatorial optimization problems be extended to multi-robot sequential assignment planning? In addition to the challenge of achieving near-optimal performance in large problems, transferability to an unseen number of robots and tasks is another key challenge for real-world applications. In this paper, we suggest a method that achieves the first success in both challenges for robot/machine scheduling problems. Our method comprises of three components. First, we show a robot scheduling problem can be expressed as a random probabilistic graphical model (PGM). We develop a mean-field inference method for random PGM and use it for Q-function inference. Second, we show that transferability can be achieved by carefully designing two-step sequential encoding of problem state. Third, we resolve the computational scalability issue of fitted Q-iteration by suggesting a heuristic auction-based Q-iteration fitting method enabled by transferability we achieved. We apply our method to discrete-time, discrete space problems (Multi-Robot Reward Collection (MRRC)) and scalably achieve 97% optimality with transferability. This optimality is maintained under stochastic contexts. By extending our method to continuous time, continuous space formulation, we claim to be the first learning-based method with scalable performance among multi-machine scheduling problems; our method scalability achieves comparable performance to popular metaheuristics in Identical parallel machine scheduling (IPMS) problems

Facing a Modern International Issue: The Politics of Biological Warfare in the United States and Korea

Biological warfare, which is the deliberate release of infectious toxins or organisms that cause diseases, has historically been a major issue that the international community has strived to resolve. By analyzing peer-reviewed journals and pre-modern documents, this research project challenges the effectiveness of pre-established policies and collaborative efforts to prevent the development and usage of biological warfare. The modern perception of biological warfare is misinterpreted because it has adapted different connotations compared to its origins. The project will engage in a historical examination of the Biological Weapons Convention and historization of major political events, such as the Korean War, to analyze how biological warfare has been characterized and how the policies have been introduced. Reflecting and revisiting the modern biological warfare narrative through inspection of its historical role along with its policies will establish an understanding that biological warfare is a modern issue and must be resolved in new terms.https://orb.binghamton.edu/research_days_posters_2023/1040/thumbnail.jp