Extracting Dynamic Information from EXAFS: Simultaneous Analysis of Multiple Temperature-Dependent Data

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/73509/1/S0909049598004336.pd

Structure and Dynamics of Metalloproteins in Live Cells

X-ray absorption spectroscopy (XAS) has emerged as one of the premier tools for investigating the structure and dynamic properties of metals in cells and in metal containing biomolecules. Utilizing the high flux and broad energy range of X-rays supplied by synchrotron light sources, one can selectively excite core electronic transitions in each metal. Spectroscopic signals from these electronic transitions can be used to dissect the chemical architecture of metals in cells, in cellular components and in biomolecules at varying degrees of structural resolution. With the development of ever-brighter X-ray sources, X-ray methods have grown into applications that can be utilized to provide both a cellular image of relative distribution of metals throughout the cell as well as a high-resolution picture of the structure of the metal. As these techniques continue to grow in their capabilities and ease of use, so to does the demand for their application by chemists and biochemists interested in studying the structure and dynamics of metals in cells, in cellular organelles and in metalloproteins

NSLS X-19A beamline performance for X-ray absorption measurements

Characterization of the X-19 beamline at the National Synchrotron Light Source (NSLS) is described. The beamline is designed for high-resolution X-ray absorption spectroscopy over a wide energy range. All of the beamline optical components are compatible with ultrahigh vacuum (UHV) operation. This permits measurements to be made in a windowless mode, thereby facilitating lower energy (Z elements (e.g., S) in highly dilute systems. The future use of this beamline for carrying out various X-ray absorption experiments is presented.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/28566/1/0000368.pd

Grazing‐angle characterization of photosynthetic oxygen evolution protein monolayers

Variable‐period x‐ray standing wave (XSW) spectroscopy has been shown to be a practical probe for studying metalloproteins. The photosynthetic oxygen evolving complex (OEC) is a transmembrane multipolypeptide complex that catalyzes the oxidation of water to dioxygen. The OEC contains Mn, Ca, and Cl and is potentially amenable to study by XSW. In this feasibility study, preliminary results on OEC samples deposited on Au mirrors are discussed. First XSW measurements from the SSRL grazing‐incidence setup are presented. © 1996 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/70867/2/RSINAK-67-9-3364-5.pd

CMR2009: 5.04: Post-mortem analysis of gadolinium distribution in NSF subjects

No Abstract.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/64915/1/323_ftp.pd

XAS of the MerR metalloregulatory protein

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27900/1/0000320.pd

Structural characterization of reduced and chemically substituted derivatives of the MN cluster in the photosynthetic oxygen evolving complex

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/30718/1/0000364.pd

Polarized XANES of iron porphyrins

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27901/1/0000321.pd

XAS investigation of the Fe sites in phthalate dioxygenase

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27899/1/0000319.pd