STUDY THE EFFECT OF MYCOPLASMA CONTAMINATION OF EGGS USED IN VIRUS TITRATION AND EFFICACY OF SOME LIVE ATTENUATED POULTRY VIRAL VACCINES

Objective: The study of Mycoplasma gallisepticum (MG) infection is needed, not only to understand the disease process but also to understand theinterference with the evaluation of some live viral poultry vaccines. This study aims to investigate the titration and potency of some live attenuatedpoultry viral vaccines; Newcastle disease, infectious bronchitis, infectious bursal disease, and Reo in both specific pathogen-free (SPF) embryonatedchicken eggs (ECEs) and chickens.Methods: Titration of live attenuated viral poultry vaccines in ECEs was carried out by dividing the inoculated eggs into four groups; the pre-,simultaneously-, post-, and non-MG contaminated. MG effect on the potency test was carried out using seventeen groups of SPF chickens (25 chicken/group) placed into separate isolators. Each live attenuated viral poultry vaccine was inoculated into 4 groups.Results: The highest titer of these vaccines that appeared in MG pre- contaminated ECEs were 1011, 107.5, 107.9, and 10, respectively. The lowest vaccinetiters that appeared in non-MG contaminated ECEs were 108, 106, 106.8, and 1067.5, respectively. Although the potency of these previous vaccines indicated thatthe highest antibodies titer that appeared in MG pre-infected vaccinated chickens were 7.5 log, 36 enzyme-linked immunosorbent assay unit (EU), and42 EU, respectively; the lowest antibodies titer that appeared in non-MG infected vaccinated chickens were 6.5 log22, 12 EU, 17 EU, and 10 EU, respectively.Conclusion: The present study findings underline the importance of using Mycoplasma -free eggs or chicken for the production of virus vaccines.Keywords: Mycoplasma gallisepticum, Newcastle disease virus, Infectious bronchitis virus, Infectious bursal disease virus, Reo virus, Chicken, Specificpathogen-free eggs. Keywords: Mycoplasmagallisepticum,Newcastlediseasevirus,Infectiousbronchitisvirus,Infectiousbursaldiseasevirus,Reovirus,Chicken,Specific pathogen-free eggs.Â

Zoonotic risk and public health hazards of companion animals in the transmission of Helicobacter species

Objective: Helicobacteriosis is worldwide infection caused by Helicobacter species that affects both humans and animals. The current work correlated the zoonotic and public health repertoire of Helicobacter species in companion animals (dogs and cats). Methods: Samples were collected from apparently healthy dogs (70), cats (65), and 70 human patients who had been in contact with these animals in the Cairo and Giza governorates. The samples included serum, feces, and stool samples and biopsies of gastric fundus fragments (~5 mm). All samples were examined by culture, biochemical analysis, serology, and molecular identification. Results: Helicobacter species were detected at a rate of 43.4% by PCR. H. heilmannii was more predominant, with a rate of 16%, whereas H. pylori was detected at 6%. H. pylori and H. heilmannii were isolated from both human and companion samples, whereas all samples were negative for H. felis. Conclusion: Dogs and cats were reservoirs and played a major source in human helicobacters infection