Responses to water stress extremes in diverse red clover germplasm accessions

Red clover (Trifolium pratense L.), a key perennial pastoral species used globally, can strengthen pastural mixes to withstand increasingly disruptive weather patterns from climate change. Breeding selections can be refined for this purpose by obtaining an in-depth understanding of key functional traits. A replicated randomized complete block glasshouse pot trial was used to observe trait responses critical to plant performance under control (15% VMC), water deficit (5% VMC) and waterlogged conditions (50% VMC) in seven red clover populations and compared against white clover. Twelve morphological and physiological traits were identified as key contributors to the different plant coping mechanisms displayed. Under water deficit, the levels of all aboveground morphological traits decreased, highlighted by a 41% decrease in total dry matter and 50% decreases in both leaf number and leaf thickness compared to the control treatment. An increase in root to shoot ratio indicated a shift to prioritizing root maintenance by sacrificing shoot growth, a trait attributed to plant water deficit tolerance. Under waterlogging, a reduction in photosynthetic activity among red clover populations reduced several morphological traits including a 30% decrease in root dry mass and total dry matter, and a 34% decrease in leaf number. The importance of root morphology for waterlogging was highlighted with low performance of red clover: there was an 83% decrease in root dry mass compared to white clover which was able to maintain root dry mass and therefore plant performance. This study highlights the importance of germplasm evaluation across water stress extremes to identify traits for future breeding programs

Evaluation of White Clover Breeding Lines in the Australasian Region

The accuracy of predicting breeding line performance across target environments is a significant criterion in the development of cultivars with broad or specific adaptation. This paper characterises the type and magnitude of genotype-by-environment (GE) interactions estimated from a multi-site white clover (Trifolium repens L.) breeding line evaluation trial conducted across sites in New Zealand and Australia

Dissecting Drought-Response Strategies of Perennial Ryegrass (\u3cem\u3eLolium perenne\u3c/em\u3e L.)

Periodic drought is a severe constraint on the profitability and sustainability of pastoral production. As a result of climate change, drought events are anticipated to increase in frequency and intensity even in regions where annual precipitation is unchanged, as well as where it is reduced. Many studies have been carried out on drought resistance of forage species (Holloway-Phillips and Brodribb 2011). However, for most experiments, it is unclear which mechanism(s) are responsible for variation in plant performance under moisture stress, whether drought response mechanisms were triggered, or whether differences in performance were simply the result of intrinsic plant vigour. The objective of the reported research was to understand the underlying physiological and genetic mechanisms responsible for production of perennial ryegrass under summer drought and recovery and regrowth after drought

Seasonal Performance of White Clover in Mixed-Sward Grazing Pasture Highlights Genotype by Environment Interaction

White clover is an important forage crop because of its nutritional value, ability to provide plantavailable nitrogen via symbiosis with Rhizobium soil bacteria, and year-round availability of dry matter (DM) yield. However, its performance in mixed sward-based pastures is characterised by seasonal variability and declining DM yield over time. The identification of white clover genotypes adapted for across seasonal performance is an important goal in white clover breeding. In this study, we evaluated the seasonal performance of 200 white clover half-sib families using visual growth scores and calibrated dry matter yield based on growth scores measured for three years in two locations. Results showed significant variation for growth scores across years, seasons and locations. Significant G×E was observed in the form of year, location and season interactions. Calibrated DM yield was highest in the second-year summer with clover content declining in the third year. Spring and winter were identified as potential vulnerable periods for white clover growth in pastures

Tradeoff between Biomass and Flavonoid Accumulation in White Clover Reflects Contrasting Plant Strategies

An outdoor study was conducted to examine relationships between plant productivity and stress-protective phenolic plant metabolites. Twenty-two populations of the pasture legume white clover were grown for 4½ months during spring and summer in Palmerston North, New Zealand. The major phenolic compounds identified and quantified by HPLC analysis were glycosides of the flavonoids quercetin and kaempferol. Multivariate analysis revealed a trade-off between flavonoid accumulation and plant productivity attributes. White clover populations with high biomass production, large leaves and thick tap roots showed low levels of quercetin glycoside accumulation and low quercetin:kaempferol ratios, while the opposite was true for less productive populations. The latter included stress-resistant ecotypes from Turkey and China, and the analysis also identified highly significant positive relationships of quercetin glycoside accumulation with plant morphology (root:shoot ratio). Importantly, a high degree of genetic variation was detected for most of the measured traits. These findings suggest merit for considering flavonoids such as quercetin as potential selection criteria in the genetic improvement of white clover and other crops

Genotypic variation for stolon and other morphological attributes of white clover (Trifolium repens L.). Populations and their influence on herbage yield in the summer rainfall region of New South Wales

A random sample of 60 germplasm accessions from the Australian white clover germplasm collection was characterized in the field at Glen Innes, N.S.W. Genotypic variation for stolon and other morphological attributes was measured in one season. Herbage yield was evaluated over seven seasons to quantify the magnitude of accession-by-season interaction. There were significant (P < 0.01) differences among accessions, seasons, and accession-by-season interactions for herbage yield. Classification was used to group the accessions on their seasonal herbage yield. A summer active group, a winter active group and a group showing autumn activity for herbage yield were identified. There was significant (P < 0.01) variation among accessions for all plant attributes measured and their accession mean repeatability was moderate to high. There were strong genotypic correlations among the morphological attributes. Five of the morphological attributes (stolon density, stolon branching, plant spread, plant height, stolon thickness and leaf length) expressed a strong genotypic correlation with average herbage yield over seven seasons. The genotypic variation estimated for herbage production and plant attributes signifies the potential of the collection to be used as a source of variation for the genetic improvement of productivity and perenniality of white clover for Australian environments

Evaluation of white clover (Trifolium repens L.) populations for summer moisture stress adaptation in Australia

Agenetic experiment was conducted using 80 full-sib families in irrigated and dryland treatments under the summer moisture stress conditions of the Northern Tablelands of New South Wales, over 3 years. This paper reports on the effects of climatic and soil moisture conditions, the genetic variation for stolon attributes and seasonal herbage yield, and the development of new recombinant genotypes in relation to the association between stolon attributes and herbage yield. Large components of variance were estimated for genotype-by-environment-by-year interactions for the attributes stolon density, number of branches, number of nodes, number of rooted nodes, stolon thickness, root diameter, internode length, and summer herbage yield. The combined analysis of variance across environments and years indicated the presence of genetic variation for the stolon attributes stolon density, number of branches, number of nodes, stolon thickness, internode length, and herbage yield. Crossing of the morphologically contrasting cultivars El Lucero x Tahora x Duron, and Barbian x El Lucero, resulted in generating genotypic recombinants with new associations between herbage yield and stolon density, number of branches, number of nodes, and number of rooted nodes. Evaluation of the full-sib families and check cultivars (cvv. Haifa and Huia) identified 5 full-sib families with relatively higher herbage yield, stolon density, number of branches, number of nodes, and number of rooted nodes than cultivars Haifa and Huia