246 research outputs found
The detection of Helicobacter pylori in paraffin sections using the PCR technique and various primers as compared to histological techniques
Helicobacter pylori is thought to represent a significant etiopathogenic factor in
diseases of the upper gastrointestinal tract. It seems, therefore, important to elaborate
effective techniques for its detection. The aim of the present study was to
evaluate the effectiveness of Helicobacter pylori detection using the PCR technique
on paraffin sections with various pairs of primers and to compare the results
with those of a histological appraisal. Material for the studies involved 50 paraffin
blocks with gastric mucosa biopsies fixed in 4% buffered formalin. In this material
4 tests were performed with the aim of diagnosing Helicobacter pylori infection:
1) H+E staining, 2) staining by the Giemsa technique, 3) an immunocytochemical
technique with antibodies against H. pylori and 4) the PCR technique with various
primers. In the present study the most reliable results for H. pylori detection as
well as the most pronounced correlation were obtained by using the PCR technique
with primers for the ureC gene, immunohistochemistry and staining according
to Giemsa. Less compatible results were obtained employing the two PCR
techniques which utilise various primers. The experiments confirmed the usefulness
of the PCR technique in the detection of Helicobacter pylori in paraffin sections
by using a suitable pair of primers, and also indicated that Giemsa staining
and immunohistochemistry should be taken into account
Elevated nuclear S100P expression is associated with poor survival in early breast cancer patients
Respiratorni simptomi u radnica na preradi riba na obali Jadrana u Hrvatskoj
This article describes respiratory symptoms and lung function in 98 fish processing female workers employed in a fish processing plant located on the Croatian Adriatic coast and 95 matching controls. The study included chronic and acute respiratory symptoms which developed during the shifts. Lung function measurements included forced vital capacity (FVC), one-second forced expiratory volume (FEV1) and maximal expiratory rates at 50 % and the last 25 % (FEF50, FEF25). Chronic respiratory symptoms were significantly dominant in fish processing workers compared to controls. The most common chronic symptoms were hoarseness (57.1 %), nasal catarrh (51.0 %), chronic cough (42.9 %), chronic phlegm (34.7 %), and frequent chest cold (35.7 %). Exposed smokers and nonsmokers had a similar prevalence of chronic respiratory symptoms. Acute symptoms over the work shift were high, with headache in lead (smokers: 62.5 %; nonsmokers: 56.1 %). Most of the ventilatory capacity parameters were significantly lower than predicted, FEF25 in particular, indicating obstructive changes predominantly in the smaller airways. These findings suggest that fish processing workers are prone to developing acute and chronic respiratory symptoms as well as to lung function changes. This calls for medical and technical preventive measures to be introduced in the work environment of the fi sh processing plant.Cilj je ovoga istraživanja bio ispitati respiratorne simptome i plućnu funkciju radnica zaposlenih na preradi riba u industriji na obali Jadranskog mora u Hrvatskoj. U istraživanje je uključeno 98 radnica zaposlenih na preradi riba i 95 žena neizložene kontrolne skupine. Ispitivani su kronični i akutni respiratorni simptomi koji se razvijaju tijekom radne smjene. Mjerena je plućna funkcija registriranjem forsiranoga vitalnog kapaciteta (FVC), forsiranoga ekspiracijskog volumena u prvoj sekundi (FEV1) te maksimalnoga ekspiracijskog protoka pri 50 % i zadnjih 25 % forsiranoga vitalnog kapaciteta (FEF50, FEF25) na krivulji maksimalni ekspiracijski protok-volumen (MEPV). Učestalost većine kroničnih respiracijskih simptoma bila je značajno viša u eksponiranih u usporedbi s kontrolnom skupinom. U eksponiranih radnica utvrđena je i visoka prevalencija akutnih simptoma koji se razvijaju tijekom radne smjene, posebno za promuklost (57,1 %) i katar nosa (51 %), potom slijedi kronični kašalj (42,9 %), kronični iskašljaj (34,7 %), upale sinusa (32 %) i česte prehlade (35,7 %). S obzirom na naviku pušenja pušači i nepušači imali su sličnu prevalenciju kroničnih respiratornih simptoma. Izložene radnice imale su visoku prevalenciju akutnih simptoma tijekom radne smjene i to naročito za glavobolju (pušači 62,5 %; nepušači 56,1 %). Ventilacijska funkcija pluća bila je značajno smanjena u usporedbi s predviđenim normalnim vrijednostima posebice za FEF25 % upućujući na opstruktivne promjene pretežno u manjim dišnim putovima. Naši podaci upućuju na opasnost razvoja kroničnih i akutnih respiracijskih simptoma i promjena plućne funkcije u radnika koji rade u industriji na preradi riba. Medicinske i tehničke preventivne mjere u radnom okolišu treba preporučiti u industriji prerade riba
The Distribution of Quasars and Galaxies in Radio Color-Color and Morphology Diagrams
We positionally match the 6 cm GB6, 20 cm FIRST and NVSS, and 92 cm WENSS
radio catalogs and find 16,500 matches in ~3,000 deg2 of sky. Using this
unified radio database, we construct radio "color-magnitude-morphology"
diagrams and find that they display a clear structure, rather than a random
scatter. We propose a simple, yet powerful, method for morphological
classification of radio sources based on FIRST and NVSS measurements. For a
subset of matched sources, we find optical identifications using the SDSS Data
Release 1 catalogs, and separate them into quasars and galaxies. Compact radio
sources with flat radio spectra are dominated by quasars, while compact sources
with steep spectra, and resolved radio sources, contain substantial numbers of
both quasars and galaxies.Comment: 4 pages, 2 figures, to be published in the Proceedings of
"Multiwavelength AGN Surveys", Cozumel, Dec 8 - 12, 200
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