NIOSOMES AS AN EMERGING FORMULATION TOOL FOR DRUG DELIVERY-A REVIEW

Nonionic surfactant based vesicles which are uni/multilamellar in structures are called niosomes. These vesicles contains an aqueous interior surrounded by one or more amphiphilic bilayer membrane forming surfactant which separates them from the bulk solution, and are also called as supramolecular aggregates. Niosomes, being an efficient drug delivery system, investigations are carried out to utilize this system to treat various disorders, to promote improved patient compliance, lesser side effects, reduction in dose, lesser dosage frequency, and higher amount of the drug at the particular site so as to lessen an excessive contact with the whole body. The Pharmacokinetic and Pharmacodynamic profile of Niosomal drug delivery system vary for various entrapped drugs. Drugs that are successful in the mitigation or treatment of CNS disorders should cross the BBB to reach the brain, as BBB seems to be an obstacle for a large number of drugs, including CNS active drugs. This article compiles recent techniques for the preparation and characterization of niosomes, the effect of formulation variables on its physicochemical properties and discussed about its effective applications in drug delivery

Fabrication and characterization of chitosan nanoparticles and collagen-loaded polyurethane nanocomposite membrane coated with heparin for atrial septal defect (ASD) closure

Atrial septal defect (ASD) constitutes 30–40% of all congenital heart diseases in adults. The most common complications in the treatment of ASD are embolization of the device and thrombosis formation. In this research, an occluding patch was developed for ASD treatment using a well-known textile technology called electrospinning. For the first time, a cardiovascular occluding patch was fabricated using medical grade polyurethane (PU) loaded with bioactive agents namely chitosan nanoparticles (Cn) and collagen (Co) which is then coated with heparin (Hp). Fourier transform infrared spectrum showed characteristic vibrations of several active constituents and changes in the absorbance due to the inclusion of active ingredients in the patch. The contact angle analysis demonstrated no significant decrease in contact angle compared to the control and the composite patches. The structure of the electrospun nanocomposite (PUCnCoHp) was examined through scanning electron microscopy. A decrease in nanofiber diameter between control PU and PUCnCoHp nanocomposite was observed. Water uptake was found to be decreased for the PUCnCoHp nanocomposite against the control. The hemocompatibility properties of the PUCnCoHp ASD occluding patch was inferred through in vitro hemocompatibility tests like activated partial thromboplastin time (APTT), prothrombin time (PT) and hemolysis assay. It was found that the PT and APTT time was significantly prolonged for the fabricated PUCnCoHp ASD occluding patch compared to the control. Likewise, the hemolysis percentage was also decreased for the PUCnCoHp ASD patch against the control. In conclusion, the developed PUCnCoHp patch demonstrates potential properties to be used for ASD occlusion

Fabrication and hemocompatibility assessment of novel polyurethane-based bio-nanofibrous dressing loaded with honey and Carica papaya extract for the management of burn injuries

Management of burn injury is an onerous clinical task since it requires continuous monitoring and extensive usage of specialized facilities. Despite rapid improvizations and investments in burn management, >30% of victims hospitalized each year face severe morbidity and mortality. Excessive loss of body fluids, accumulation of exudate, and the development of septic shock are reported to be the main reasons for morbidity in burn victims. To assist burn wound management, a novel polyurethane (PU)-based bio-nanofibrous dressing loaded with honey (HN) and Carica papaya (PA) fruit extract was fabricated using a one-step electrospinning technique. The developed dressing material had a mean fiber diameter of 190±19.93 nm with pore sizes of 4-50 µm to support effective infiltration of nutrients and gas exchange. The successful blending of HN- and PA-based active biomolecules in PU was inferred through changes in surface chemistry. The blend subsequently increased the wettability (14%) and surface energy (24%) of the novel dressing. Ultimately, the presence of hydrophilic biomolecules and high porosity enhanced the water absorption ability of the PU-HN-PA nanofiber samples to 761.67% from 285.13% in PU. Furthermore, the ability of the bio-nanofibrous dressing to support specific protein adsorption (45%), delay thrombus formation, and reduce hemolysis demonstrated its nontoxic and compatible nature with the host tissues. In summary, the excellent physicochemical and hemocompatible properties of the developed PU-HN-PA dressing exhibit its potential in reducing the clinical complications associated with the treatment of burn injuries

Analyzing the trade competitiveness of Indian coconut sector in the liberalization regime

Coconut export sector in India of late gained an outward orientation, especially since the Coconut Development Board was elevated to the status of export promotion council in the year 2009. In the recent period, the growth rate experienced in the coconut based value added products has been stupendous. Since there is an attempt for global value chain up-gradation at the production node of the sector, it is imperative to analyse our strengths in the world market in comparison with the major competitors. We have used the Revealed Comparative Advantage (RCA) methodology to analyse India’s competitiveness in the exports of major coconut value added products traded across the world. The study revealed that, as far as the coconut value added products are concerned, India is comparatively a very small player with paltry export market shares. The analysis of RCA revealed that comparative advantage of India is lower than the major coconut exporting countries like Philippines, Indonesia and Sri Lanka. The Philippines is the dominant player with highest RCA indices in most of the coconut value added product lines. The study suggests the need to formulate plausible strategies to reach the overseas market and capture the optimal share in market segments. Though we have a strong domestic market base, it is an indubitable fact that in the near future due to the evolving trade agreements even in the domestic sector we may confront fierce price competition from the overseas imports. Hence we need to chalk out modalities and execution plans to elevate our export competitiveness and comparative advantage

A robust estimate for location in experiments with plantation crops

Presence of outliers or extreme values in the experimental data is a major concern for data analysis. Many times, the experimental data contains abnormal or extreme values due to genetic variations, loss of yield due to pest/disease infestation, errors in tabulation/data entry etc. In field experimental data analysis, the plot mean or treatment mean is usually considered for comparison. The sample mean, which is usually taken as an estimate of the population mean (location estimator) is highly affected by the presence of outliers or extreme values particularly when the sample size is small. In this paper, kernel weighted location estimator with weight proportional to the value of the estimated kernel density function is proposed, to handle the outliers or extreme values. The kernel weighted location estimator is robust even if the underlying distribution is non-symmetric. The performance of the proposed method is compared with the existing procedures through simulation study. The method is also applied to the analysis of yield data of arecanut and cocoa mixed cropping experiment at CPCRI Regional Station, Vittal

Group approach for enhancing profitability of small holders through technology integration-reflections from coconut farming

Technology integration for enhancing productivity and income from coconut farming, facilitated through stakeholder participation, was a major objective of the NAIP sub-project on ‘Value chain in coconut’ implemented in a consortium mode with ICAR-Central Plantation Crops Research Institute, Kasaragod as the lead institute. To achieve this objective, 10 clusters of coconut farmers were formed under the project in selected panchayats of Kasaragod district in Kerala state. A total of 534 farmers covering 250 ha participated in the project interventions. Appropriate production technologies were integrated in their holdings which included intercropping, growing of leguminous crops in the coconut basin, organic recycling through vermicomposting and integrated nutrient management. Group action was initiated among the farmers for taking up need-based integrated disease management measures especially to control bud rot disease of coconut. Knowledge and skill upgradation of farmers on the selected technologies were achieved through various institutional and off campus training programmes. The net income from the coconut farming in small and marginal holdings could be increased through the interventions under the project. Technology integration in the holdings also resulted in the increase of coconut productivity from 60 to 112 nuts per palm. Technology integration in small and marginal coconut holdings for higher productivity and income through Community Based Organizations approach is scaled up by other agencies like Coconut Development Board for implementing development/extension programmes

Analysis of organic farming practices in cocoa in India

The study was conducted among 120 cocoa growers drawn randomly from one district each from Tamil Nadu, Kerala, Andhra Pradesh and Karnataka states. Profile characteristics of farmers were quantified based on socio-personal, psychological and economic variables. Majority of farmers were middle aged (64.2%), literate (96%), with less than 2 ha area under cocoa (64%) and farmers having livestock (77%). Most of the farmers had medium level of social participation, extension orientation and mass media exposure. One fifth of farmers had undergone training on organic farming, soil testing was done by nearly 31 per cent farmers and very few farmers (5%) had done organic certification. Mulching and use of green leaf manure were the main agronomic practices. Main organic inputs which were produced or prepared at the farm were, farm yard manure, crop residues, cow dung slurry, ash and vermicompost. Farm yard manure, neem cake, biofertilizers, goat manure etc. were the major inputs which were purchased from the market. Majority of cocoa growers (68.3%) had medium level of knowledge about organic farming practices. Knowledge of farmers on biocontrol agents (35%), botanical pesticides (33.3%) and biofertilizers (31.7%) was recorded. Variables like, education, social participation, extension orientation, mass media exposure and training attended were found to have positive and significant relationship with knowledge on organic farming practices. The mean yield and productivity of cocoa was found to be 1.2 kg dry beans tree-1year-1 and 608 kg dry beans ha-1 respectively. Gross income from cocoa was calculated as `170 tree-1year-1 and ` 83377 ha-1. Non-availability of labour, non-availability of quality organic inputs, difficulty in controlling pests and diseases by organic methods and lack of knowledge about organic farming practices were the major constraints

UV induced surface modification on improving the cytocompatibility of metallocene polyethylene

Demand for medical implants is rising day by day as the world becomes the place for more diseased and older people. Accordingly, in this research, metallocene polyethylene (mPE), a commonly used polymer was treated with UV rays for improving its biocompatibility. Scanning electron microscopy (SEM) images confirmed the formation of crests and troughs, which depicts the improvement of surface roughness of mPE substrates caused by UV etching. Accordingly, the contact angle measurements revealed that the wettability of mPE-2.5 J/cm2 (68.09º) and mPE-5 J/cm2 (57.93º) samples were found to be increased compared to untreated mPE (86.84º) indicating better hydrophilicity. Further, the UV treated surface exhibited enhanced blood compatibility as determined in APTT (untreated mPE- 55.3 ± 2.5 s, mPE-2.5 J/ cm2 - 76.7 ± 4.1 s and mPE-5 J/cm2 - 112.3 ± 2 s) and PT (untreated mPE - 24.7 ± 1.5 s, mPE- 2.5 J/cm2 - 34.3 ± 1.1 s and mPE-5 J/cm2 - 43 ± 2 s) assay. Moreover, the treated mPE-2.5 J/cm2 (4.88%) and mPE-5 J/cm2 (1.79%) showed decreased hemolytic percentage compared to untreated mPE (15.40%) indicating better safety to red blood cells. Interestingly, the changes in physicochemical properties of mPE are directly proportional to the dosage of the UV rays. UV modified mPE surfaces were found to be more compatible as identified through MTT assay, photomicrograph and SEM images of the seeded 3T3 cell population. Hence UV-modified surface of mPE may be successfully exploited for medical implants

Design and Implementation of Improved Electronic Load Controller for Self-Excited Induction Generator for Rural Electrification

This paper offers an alternative technique, namely, Improved Electronic Load Controller (IELC), which is proposal to improve power quality, maintaining voltage at frequency desired level for rural electrification. The design and development of IELC are considered as microhydroenergy system. The proposed work aims to concentrate on the new schemes for rural electrification with the help of different kinds of hybrid energy systems. The objective of the proposed scheme is to maintain the speed of generation against fluctuating rural demand. The Electronic Load Controller (ELC) is used to connect and disconnect the dump load during the operation of the system, and which absorbs the load when consumer are not in active will enhance the lifestyle of the rural population and improve the living standards. Hydroelectricity is a promising option for electrification of remote villages in India. The conventional methods are not suitable to act as standalone system. Hence, the designing of a proper ELC is essential. The improved electronic load control performance tested with simulation at validated through hardware setup

Gallic acid induced apoptotic events in HCT-15 colon cancer cells

AIM: To investigate the inhibitory action of diet-derived phenolic compound gallic acid (GA) against HCT-15 colon cancer cells. METHODS: The antiproliferative effect of GA against colon cancer cells was determined by performing thiazolyl blue tetrazolium bromide (MTT) assay. The colony forming ability of GA treated colon cancer cells was evaluated using the colony forming assay. The cell cycle changes induced by GA in HCT-15 cells were analyzed by propidium iodide staining. Levels of reactive oxygen species (ROS) and mitochondrial membrane potential of HCT-15 exposed to GA was assessed using 2',7'-dichlorfluorescein-diacetate and rhodamine-123 respectively, with the help of flow cytometry. Morphological changes caused by GA treatment in the colon cancer cells were identified by scanning electron microscope and photomicrograph examination. Apoptosis was confirmed using flow cytometric analysis of GA treated HCT-15 cells after staining with Yo-Pro-1. RESULTS: MTT assay results illustrated that GA has an inhibitory effect on HCT-15 cells with IC50 value of 740 μmol/L. A time-dependent inhibition of colony formation was evident with GA treatment. Cell cycle arrest was evident from the accumulation of GA treated HCT-15 cells at sub-G1 phase (0.98 ± 1.03 vs 58.01 ± 2.05) with increasing exposure time. Flow cytometric analysis of GA treated HCT-15 cells depicted early events associated with apoptosis like lipid layer breakage and fall in mitochondrial membrane potential apart from an increase in the generation of ROS which were in a time dependent manner. SEM and photomicrograph images of the GA-treated cells displayed membrane blebbing and cell shrinking characteristics of apoptosis. Further apoptosis confirmation by Yo-Pro-1 staining also showed the time-dependent increase of apoptotic cells after treatment. CONCLUSION: These results show that GA induced ROS dependent apoptosis and inhibited the growth of colon cancer cells