Self-adaptation and viscous selection in concentrated two-dimensional vortex dipoles

International audienceIn this Letter we deal with 2D direct numerical simulations of concentrated vortex dipoles. We show that various initial dipolar vorticity distributions evolve towards a specific family of dipoles parametrized by the dipole aspect ratio a/b, where a is the radius of the vortices based on the vorticity polar moment in half a plane and b is the separation between the vortex centroids. This convergence is achieved through viscous effects. The considered Reynolds numbers Re= G/v are Re= 3000 and Re= 15000. Moreover, all the dipoles of this family are quasi-steady solutions of the Euler equations. Their scatter plots and drift velocities are given for a/b<03. © 2000 American Institute of Physics

Can the jamming transition be described using equilibrium statistical mechanics?

When materials such as foams or emulsions are compressed, they display solid behaviour above the so-called `jamming' transition. Because compression is done out-of-equilibrium in the absence of thermal fluctuations, jamming appears as a new kind of a nonequilibrium phase transition. In this proceeding paper, we suggest that tools from equilibrium statistical mechanics can in fact be used to describe many specific features of the jamming transition. Our strategy is to introduce thermal fluctuations and use statistical mechanics to describe the complex phase behaviour of systems of soft repulsive particles, before sending temperature to zero at the end of the calculation. We show that currently available implementations of standard tools such as integral equations, mode-coupling theory, or replica calculations all break down at low temperature and large density, but we suggest that new analytical schemes can be developed to provide a fully microscopic, quantitative description of the jamming transition.Comment: 8 pages, 6 figs. Talk presented at Statphys24 (July 2010, Cairns, Australia

Inducible Control of Subcellular RNA Localization Using a Synthetic Protein-RNA Aptamer Interaction

Evidence is accumulating in support of the functional importance of subcellular RNA localization in diverse biological contexts. In different cell types, distinct RNA localization patterns are frequently observed, and the available data indicate that this is achieved through a series of highly coordinated events. Classically, cis–elements within the RNA to be localized are recognized by RNA-binding proteins (RBPs), which then direct specific localization of a target RNA. Until now, the precise control of the spatiotemporal parameters inherent to regulating RNA localization has not been experimentally possible. Here, we demonstrate the development and use of a chemically–inducible RNA–protein interaction to regulate subcellular RNA localization. Our system is composed primarily of two parts: (i) the Tet Repressor protein (TetR) genetically fused to proteins natively involved in localizing endogenous transcripts; and (ii) a target transcript containing genetically encoded TetR–binding RNA aptamers. TetR–fusion protein binding to the target RNA and subsequent localization of the latter are directly regulated by doxycycline. Using this platform, we demonstrate that enhanced and controlled subcellular localization of engineered transcripts are achievable. We also analyze rules for forward engineering this RNA localization system in an effort to facilitate its straightforward application to studying RNA localization more generally.National Institutes of Health (U.S.) (Director’s New Innovator Award Program (grant number 1DP2OD007124 )

Direct and adjoint global modes of a recirculation bubble: Lift-up and convective non-normalities

International audienceThe stability of the recirculation bubble behind a smoothed backward-facing step is numerically computed. Destabilization occurs first through a stationary three-dimensional mode. Analysis of the direct global mode shows that the instability corresponds to a deformation of the recirculation bubble in which streamwise vortices induce low- and high-speed streaks as in the classical lift-up mechanism. Formulation of the adjoint problem and computation of the adjoint global mode show that both the lift-up mechanism associated with the transport of the base flow by the perturbation and the convective non-normality associated with the transport of the perturbation by the base flow explain the properties of the flow. The lift-up non-normality differentiates the direct and adjoint modes by their component: the direct is dominated by the streamwise component and the adjoint by the cross-stream component. The convective non-normality results in a different localization of the direct and adjoint global modes, respectively downstream and upstream. The implications of these properties for the control problem are considered. Passive control, to be most efficient, should modify the flow inside the recirculation bubble where direct and adjoint global modes overlap, whereas active control, by for example blowing and suction at the wall, should be placed just upstream of the separation point where the pressure of the adjoint global mode is maximum. © 2009 Cambridge University Press

A statistical approach for predicting grassland degradation in disturbance-driven landscapes

Maintaining a land base that supports safe and realistic training operations is a significant challenge for military land managers which can be informed by frequent monitoring of land condition in relation to management practices. This study explores the relationship between fire and trends in tallgrass prairie vegetation at military and non -military sites in the Kansas Flint Hills. The response variable was the longterm linear trend (2001-2010) of surface greenness measured by MODIS NDVI using BFAST time series trend analysis. Explanatory variables included fire regime (frequency and seasonality) and spatial strata based on existing management unit boundaries. Several non-spatial generalized linear models (GLM) were computed to explain trends by fire regime and/or stratification. Spatialized versions of the GLMs were also constructed. For non-spatial models at the military site, fire regime explained little (4%) of the observed surface greenness trend compared to strata alone (7% - 26%). The non-spatial and spatial models for the non -military site performed better for each explanatory variable and combination tested with fire regime. Existing stratifications contained much of the spatial structure in model residuals. Fire had only a marginal effect on surface greenness trends at the military site despite the use of burning as a grassland management tool. Interestingly, fire explained more of the trend at the nonmilitary site and models including strata improved explanatory power. Analysis of spatial model predictors based on management unit stratification suggested ways to reduce the number of strata while achieving similar performance and may benefit managers of other public areas lacking sound data regarding land usage

Shared autonomic pathways connect bone marrow and peripheral adipose tissues across the central neuraxis

Bone marrow adipose tissue (BMAT) is increased in both obesity and anorexia. This is unique relative to white adipose tissue (WAT), which is generally more attuned to metabolic demand. It suggests that there may be regulatory pathways that are common to both BMAT and WAT and also those that are specific to BMAT alone. The central nervous system (CNS) is a key mediator of adipose tissue function through sympathetic adrenergic neurons. Thus, we hypothesized that central autonomic pathways may be involved in BMAT regulation. To test this, we first quantified the innervation of BMAT by tyrosine hydroxylase (TH) positive nerves within the metaphysis and diaphysis of the tibia of B6 and C3H mice. We found that many of the TH+ axons were concentrated around central blood vessels in the bone marrow. However, there were also areas of free nerve endings which terminated in regions of BMAT adipocytes. Overall, the proportion of nerve-associated BMAT adipocytes increased from proximal to distal along the length of the tibia (from ~3-5 to ~14-24%), regardless of mouse strain. To identify the central pathways involved in BMAT innervation and compare to peripheral WAT, we then performed retrograde viral tract tracing with an attenuated pseudorabies virus (PRV) to infect efferent nerves from the tibial metaphysis (inclusive of BMAT) and inguinal WAT (iWAT) of C3H mice. PRV positive neurons were identified consistently from both injection sites in the intermediolateral horn of the spinal cord, reticular formation, rostroventral medulla, solitary tract, periaqueductal gray, locus coeruleus, subcoeruleus, Barrington\u27s nucleus, and hypothalamus. We also observed dual-PRV infected neurons within the majority of these regions. Similar tracings were observed in pons, midbrain, and hypothalamic regions from B6 femur and tibia, demonstrating that these results persist across mouse strains and between skeletal sites. Altogether, this is the first quantitative report of BMAT autonomic innervation and reveals common central neuroanatomic pathways, including putative command neurons, involved in coordinating multiple aspects of sympathetic output and facilitation of parallel processing between bone marrow/BMAT and peripheral adipose tissue