6 research outputs found
Antibacterial activities of selected edible plants extracts against multidrug-resistant Gram-negative bacteria
BACKGROUND: In response to the propagation of bacteria resistant to many antibiotics also called multi-drug resistant (MDR) bacteria, the discovery of new and more efficient antibacterial agents is primordial. The present study was aimed at evaluating the antibacterial activities of seven Cameroonian dietary plants (Adansonia digitata, Aframomum alboviolaceum, Aframomum polyanthum, Anonidium. mannii, Hibiscus sabdarifa, Ocimum gratissimum and Tamarindus indica). METHODS: The phytochemical screening of the studied extracts was performed using described methods whilst the liquid broth micro dilution was used for all antimicrobial assays against 27 Gram-negative bacteria. RESULTS: The results of the phytochemical tests indicate that all tested extracts contained phenols and triterpenes, other classes of chemicals being selectively present. The studied extracts displayed various degrees of antibacterial activities. The extracts of A. digitata, H. sabdarifa, A. polyanthum, A. alboviolaceum and O. gratissimum showed the best spectra of activity, their inhibitory effects being recorded against 81.48%, 66.66%, 62.96%, 55.55%, and 55.55% of the 27 tested bacteria respectively. The extract of A. polyanthum was very active against E. aerogenes EA294 with the lowest recorded minimal inhibitory concentration (MIC) of 32 μg/ml. CONCLUSION: The results of the present work provide useful baseline information for the potential use of the studied edible plants in the fight against both sensitive and MDR phenotypes
Activities of selected medicinal plants against multi-drug resistant Gram-negative bacteria in Cameroon
Background: Medicinal plants are used worldwide for several human
ailments including bacterial infections. The present work was designed
to assess the in vitro antibacterial activities of some Cameroonian
medicinal plants including Entada abyssinica , Entada africana ,
Pentaclethra macrophylla , Allexis cauliflora , Anthocleista
leibrechtsiana , Carapa procera , Carica papaya and Persea
americana against Gram-negative bacteria expressing multidrug
resistant (MDR) phenotypes. Methods: The microbroth dilution was used
to determine the minimal inhibitory concentration (MIC) and minimal
bactericidal concentration (MBC) of the samples against eight bacterial
strains belonging to four species, Escherichia coli , Enterobacter
aerogenes , Klebsiella pneumoniae and Providencia stuartii .
Results: The extracts displayed selective antibacterial activities with
the minimal inhibitory concentrations (MIC) values ranges of 64 to 1024
μg/mL. The most active extract was that from Pentaclethra
macrophylla (TPM) that showed inhibitory activities against five of the
eight (62.5%) tested bacteria. The lowest MIC value (64 μg/mL) was
recorded with the crude extract of Entada africana against E. coli
AG100A whilst the best MBC (256 μg/mL) value was also obtained
with methanol extract of Persea americana against this bacterial
strain. Conclusion: The results of the present work provide baseline
information on the possible use of Pentaclethra macrophylla, Entada
africana and Entada abyssinica in the treatment of selected bacterial
infections