The activity of alpha-amylase in serum and some organs of guinea pigs with larval ascaridosis

The studies were carried out on 40 guinea pig males weighting about 230 g. The experimental animals were infected with 7000 invasive eggs of Ascaris suum. On the 3-rd, 7-th and 10-th day after infection the activity of alpha-amylase was estimated according to Caraway's method in serum, liver, pancreas, lungs, kidneys and spleen. The infection of guinea pigs results in increased activity of enzyme in serum and spleen, and decreased in pancreas, liver, kidneys and lungs. On the 3-rd day after infection the changes in amylase activity were the most intense

Methods of cultivating of parasitic nematodes in vitro

The review of literature referring to cultivating methods of some parasitic nematodes in vitro is presented (Ascaridia galli, Ascaris suum, Nippostrongylus brasiliensis, Strongyloides fülleborni). It refers general problems and initiate in subject of parasites cultivating in vitro. This work comprises terminology and elementary problems connected with the starting of culture of parasites in vitro, such as: obtaining of parasites to the starting of cultures, the types of cultures in vitro and forming of conditions to determine successful of culture of parasitic nematodes in vitro (sterilization, the selecting of medium, the forming of most favourable physical-chemical conditions)

The effect of low temperatures on the development of eggs of Ascaris suum Goeze, 1782

Eggs of A. suum were kept at -10 and -20'C for 12 weeks and subsequently incubated in a thermostat at 28°C. During the incubation, their development was checked every 5 days. It was found that storage at low temperatures slows down their development and reduces the number of eggs successfully completing their embryogenesis

THE ACTIVITIES OF ALPHA-AMYLASE, TRYPSIN AND LIPASE IN PANCREAS AND DUODENAL CONTENTS OF ASCARIDIA GALLI INFECTED CHICKENS FED WITH VARIOUS PROTEIN DIETS

The study was carried out on Astra S chickens which were grown on diets containing 11% and 19% proteins. In homogenized pancreas and duodenal contents from control animals and chickens infected with 500 invasive eggs of Ascaridia galli activities of alpha-amylase (FENNEL method), lipase (CHERRY-CRANDALL method) and trypsin (ANSON method) were determined. After 7 weeks of the invasion the activities of these enzymes were higher in duodenal contents and lower in pancreas of infected birds in comparison with the control animals. The differences were significant for alpha-amylase and lipase activities in animals which were given 11% protein diet, and for trypsin activity of chickens groups fed with 19% protein diet