2 research outputs found

    Isolation, Screening and Identification of Swine Gut Microbiota with Ochratoxin A Biodegradation Ability

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    The potential for ochratoxin A (OTA) degradation by swine intestinal microbiota was assessed in the current study. Intestinal content that was collected aseptically from swine was spiked with 100 ppb OTA and incubated for 6 and 12 h at 39ยฐC. An OTA assay was conducted using the incubated samples, and it was found that 20% of the OTA toxin was detoxified, indicating the presence of microbes capable of OTA degradation. Twenty-eight bacterial species were isolated anaerobically in M 98-5 media and 45 bacterial species were isolated using nutrient broth aerobically. Screening results showed that one anaerobic bacterial isolate, named MM11, detoxified more than 75% of OTA in liquid media. Furthermore, 1.0 ppm OTA was degraded completely after 24 h incubation on a solid โ€˜cornโ€™ substrate. The bacterium was identified by 16S rDNA sequencing as having 97% sequence similarity with Eubacterium biforme. The isolation of an OTA-degrading bacterium from the swine natural flora is of great importance for OTA biodegradation and may be a valuable potential source for OTA-degradation enzymes in industrial applications

    The Korean Journal of Pathology์˜ SCI-e ๋ฐ JCR ๋“ฑ์žฌ๋ฅผ ์ถ•ํ•˜ํ•ฉ๋‹ˆ๋‹ค

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    2008๋…„ 5์›” Thomson & Reuters (Thomson Scientific Co.)๋Š” The Korean Journal of Pathology (Korean J Pathol) ๋“ฑ 9๊ฐœ ํ•œ๊ตญ ํ•™์ˆ ์ง€๋ฅผ ํฌํ•จํ•œ 157๊ฐœ ํ•™์ˆ ์ง€๋ฅผ ์ƒˆ๋กญ๊ฒŒ SCI-expanded์— ๋“ฑ์žฌํ•˜๊ณ  3๊ฐœ ํ•™์ˆ ์ง€๋ฅผ ์ œ์™ธํ•œ๋‹ค๊ณ  ๋ฐœํ‘œํ•˜์˜€๋‹ค. 2008๋…„ 5์›” ํ˜„์žฌ ์ „์ฒด SCI-e ํ•™์ˆ ์ง€๋Š” ๋ชจ๋‘ 7,321๊ฐœ์ด๋ฉฐ ์ด ์ค‘ ํ•œ๊ตญ์—์„œ ๋ฐœํ–‰๋˜๋Š” ํ•™์ˆ ์ง€๋Š” ์ด 59๊ฐœ์ด๊ณ  ๊ทธ ์ค‘ ์˜ํ•™ ์ƒ๋ช…๊ณผํ•™ ๋ถ„์•ผ ํ•™์ˆ ์ง€๋Š” 12๊ฐœ์ด๋‹ค. 1967๋…„ ์ฐฝ๊ฐ„ํ•œ Korean J Pathol์€ ์ด๋ฒˆ์— SCI-expanded์— ๋“ฑ์žฌ๋˜๋ฉด์„œ ๊ตญ์ œ์‚ฌํšŒ์—์„œ ์ธ์ •๋ฐ›๋Š” ์œ ์ˆ˜์˜ ์ „๋ฌธํ•™์ˆ ์ง€๊ฐ€ ๋˜์—ˆ๋‹ค. ์ด์ œ๋ถ€ํ„ฐ ์šฐ๋ฆฌ ํ•™ํšŒ์ง€์— ๊ฒŒ์žฌ๋œ ๋…ผ๋ฌธ๊ณผ ๊ทธ ๋…ผ๋ฌธ์ด ์ธ์šฉํ•œ ์ฐธ๊ณ  ๋ฌธํ—Œ์€ ์„ธ๊ณ„์˜ ๊ณผํ•™์ž๋“ค์ด ๊ฐ€์žฅ ๋งŽ์ด ๊ฒ€์ƒ‰ํ•˜๋Š” ๋ฐ์ดํ„ฐ๋ฒ ์ด์Šค์— ํฌํ•จ๋จ์œผ๋กœ์จ ๋” ์ฝํžˆ๊ณ , ๋” ์ธ์šฉ๋  ๋ฟ ์•„๋‹ˆ๋ผ ๋” ๋งŽ์€ ๊ฐ์‹œ๋ฅผ ๋ฐ›๊ฒŒ ๋œ๋‹ค. ์ด๋Š” 2008๋…„ ์ฐฝ๊ฐ„๋œ Basic and Applied Pathology1 ์™€ ๋”๋ถˆ์–ด 2008๋…„๋„ ๋Œ€ํ•œ๋ณ‘๋ฆฌํ•™ํšŒ ํ•™์ˆ  ํ™œ๋™์˜ ๊ฒฝ์‚ฌ์ด๋ฉฐ, ์ด์— SCI-e ๋“ฑ์žฌ์˜ ์˜๋ฏธ๋ฅผ ์ดํ•ดํ•˜๊ณ  ๋ฐœ์ „์ ์ธ ํ•™ํšŒ์™€ ํ•™์ˆ ์ง€ ์šด์˜์— ๋Œ€ํ•˜์—ฌ ์ƒ๊ฐํ•ด ๋ณด๊ธฐ๋กœ ํ•œ๋‹ค
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