Nucleus-targeted Dmp1 transgene fails to rescue dental defects in Dmp1 null mice

Dentin matrix protein 1 (DMP1) is essential to odontogenesis. Its mutations in human subjects lead to dental problems such as dental deformities, hypomineralization and periodontal impairment. Primarily, DMP1 is considered as an extracellular matrix protein that promotes hydroxyapatite formation and activates intracellular signaling pathway via interacting with αvβ3 integrin. Recent in vitro studies suggested that DMP1 might also act as a transcription factor. In this study, we examined whether full-length DMP1 could function as a transcription factor in the nucleus and regulate odontogenesis in vivo. We first demonstrated that a patient with the DMP1 M1V mutation, which presumably causes a loss of the secretory DMP1 but does not affect the nuclear translocation of DMP1, shows a typical rachitic tooth defect. Furthermore, we generated transgenic mice expressing (NLS)DMP1, in which the endoplasmic reticulum (ER) entry signal sequence of DMP1 was replaced by a nuclear localization signal (NLS) sequence, under the control of a 3.6 kb rat type I collagen promoter plus a 1.6 kb intron 1. We then crossbred the (NLS)DMP1 transgenic mice with Dmp1 null mice to express the (NLS)DMP1 in Dmp1-deficient genetic background. Although immunohistochemistry demonstrated that (NLS)DMP1 was localized in the nuclei of the preodontoblasts and odontoblasts, the histological, morphological and biochemical analyses showed that it failed to rescue the dental and periodontal defects as well as the delayed tooth eruption in Dmp1 null mice. These data suggest that the full-length DMP1 plays no apparent role in the nucleus during odontogenesis

Production cross sections of superheavy nuclei based on dinuclear system model

The barrier distribution function method is introduced in the dinuclear system model in the calculation of the transmission probability, which is the first stage in the synthesis of superheavy nuclei. Dynamical deformation and averaging collision orientations are considered in the calculation of the fusion probability by solving master equation numerically. Survival probability with respect to xn evaporation channel (x = 1-5) in the de-excitation process of the thermal compound nucleus is calculated, in which the level density of the Fermi-gas model is used. Production cross sections of a series of superheavy nuclei formed in the reactions taken magic and deformed nuclei as target in Ca-48 induced reactions are studied systematically. The calculated results are in good agreement with available experimental data. Isotopic dependence of the production cross sections in the reactions Ca-48 + Pu is analyzed

Isotopic dependence of production cross sections of superheavy nuclei in hot fusion reactions

The dinuclear system model has been further developed by introducing the barrier distribution function method in the process of heavy-ion capture and fusion to synthesize superheavy nuclei. The capture of two colliding nuclei, formation and de-excitation process of compound nucleus are decribed by using empirical coupled channel model, solving master equation numerically and statistical evaporation model, respectively. Within the framework of the dinuclear system model, the fusion-evaporation excitation functions of the systems Ca-48(Am-243, 3n-5n) (288-286)115 and Ca-48(Cm-248, 3n-5n)(293-291)116 are calculated, which are used for synthesizing new superheavy nuclei at Dubna in recent years. Isotopic dependence of production cross sections with double magic nucleus Ca-48 bombarding actinide targets U, Np, Pu, Am, Cm to synthesize superheavy nuclei with charged numbers Z=112-116 is analyzed systematically. Based on these analysis, the optimal projectile-target combination and the optimal excitation energy are proposed. It is shown that shell correction energy and neutron separation energy will play an important role on the isotopic dependence of production cross sections of superheavy nuclei