Evaluating genetic markers and neurobiochemical analytes for fluoxetine response using a panel of mouse inbred strains

RationaleIdentification of biomarkers that establish diagnosis or treatment response is critical to the advancement of research and management of patients with depression.ObjectiveOur goal was to identify biomarkers that can potentially assess fluoxetine response and risk to poor treatment outcome.MethodsWe measured behavior, gene expression, and the levels of 36 neurobiochemical analytes across a panel of genetically diverse mouse inbred lines after chronic treatment with water or fluoxetine.ResultsGlyoxylase 1 (GLO1) and guanine nucleotide-binding protein 1 (GNB1) mostly account for baseline anxiety-like and depressive-like behavior, indicating a common biological link between depression and anxiety. Fluoxetine-induced biochemical alterations discriminated positive responders, while baseline neurobiochemical differences differentiated negative responders (p < 0.006). Results show that glial fibrillary acidic protein, S100 beta protein, GLO1, and histone deacetylase 5 contributed most to fluoxetine response. These proteins are linked within a cellular growth/proliferation pathway, suggesting the involvement of cellular genesis in fluoxetine response. Furthermore, a candidate genetic locus that associates with baseline depressive-like behavior contains a gene that encodes for cellular proliferation/adhesion molecule (Cadm1), supporting a genetic basis for the role of neuro/gliogenesis in depression.ConclusionWe provided a comprehensive analysis of behavioral, neurobiochemical, and transcriptome data across 30 mouse inbred strains that has not been accomplished before. We identified biomarkers that influence fluoxetine response, which, altogether, implicate the importance of cellular genesis in fluoxetine treatment. More broadly, this approach can be used to assess a wide range of drug response phenotypes that are challenging to address in human samples.Electronic supplementary materialThe online version of this article (doi:10.1007/s00213-011-2574-z) contains supplementary material, which is available to authorized users

The effects of suspended sediment on coral reef fish assemblages and feeding guilds of north-west Australia

© 2018 Springer-Verlag GmbH Germany, part of Springer Nature Understanding environmental drivers of fish assemblage composition is fundamental to designing useful management plans for anthropogenic activities. This study investigated the influence of suspended sediment on reef fish assemblages and habitat composition over a cross-shelf turbidity gradient in the nearshore Pilbara region of Western Australia. The responses of all feeding guilds were analysed to identify feeding guilds vulnerable to elevations in suspended sediment and sedimentation. This study is the first of its kind to utilise both remotely sensed and in situ turbidity data to characterise the general turbidity regime and magnitude of stochastic events. By using the coordinates from a principle coordinates analysis, we were able to capture both the general turbidity conditions and stochastic turbidity spikes at each site, whilst the use of in situ turbidity measurements allowed us to effectively ‘ground-truth’ the remotely sensed TSS data. Underwater visual census was used to sample the fish assemblage and quantify habitat across the turbidity gradient. Generalised additive mixed models were used to explore the relationships between environmental variables, habitat variables, and fish assemblage metrics. Species richness significantly declined with increasing levels of turbidity. This study identified two key functional groups of fish that are vulnerable to elevated suspended sediment loads. Planktivorous omnivores and herbivorous scrapers decreased in abundance at high turbidity sites. Total biomass of herbivorous scrapers was lower at turbid sites, whilst the biomass of planktivorous omnivores was not related to any of the predictor variables. Changes in the abundance, biomass, and behaviour of these functional groups can have severe consequences for the resilience and recovery of coral reefs. Anthropogenic activities that elevate suspended sediment in regions where coral reef communities exist should consider these implications when designing management plans