Conserved Endonuclease Function of Hantavirus L Polymerase.

Hantaviruses are important emerging pathogens belonging to the Bunyaviridae family. Like other segmented negative strand RNA viruses, the RNA-dependent RNA polymerase (RdRp) also known as L protein of hantaviruses lacks an intrinsic "capping activity". Hantaviruses therefore employ a "cap snatching" strategy acquiring short 5' RNA sequences bearing 5'cap structures by endonucleolytic cleavage from host cell transcripts. The viral endonuclease activity implicated in cap snatching of hantaviruses has been mapped to the N-terminal domain of the L protein. Using a combination of molecular modeling and structure-function analysis we confirm and extend these findings providing evidence for high conservation of the L endonuclease between Old and New World hantaviruses. Recombinant hantavirus L endonuclease showed catalytic activity and a defined cation preference shared by other viral endonucleases. Based on the previously reported remarkably high activity of hantavirus L endonuclease, we established a cell-based assay for the hantavirus endonuclase function. The robustness of the assay and its high-throughput compatible format makes it suitable for small molecule drug screens to identify novel inhibitors of hantavirus endonuclease. Based on the high degree of similarity to RdRp endonucleases, some candidate inhibitors may be broadly active against hantaviruses and other emerging human pathogenic Bunyaviruses

Coherent current transport in wide ballistic Josephson junctions

We present an experimental and theoretical investigation of coherent current transport in wide ballistic superconductor-two dimensional electron gas-superconductor junctions. It is found experimentally that upon increasing the junction length, the subharmonic gap structure in the current-voltage characteristics is shifted to lower voltages, and the excess current at voltages much larger than the superconducting gap decreases. Applying a theory of coherent multiple Andreev reflection, we show that these observations can be explained in terms of transport through Andreev resonances.Comment: 4 pages, 4 figure

Transport in metallic multi-island Coulomb blockade systems: A systematic perturbative expansion in the junction transparency

We study electronic transport through metallic multi-island Coulomb-blockade systems. Based on a diagrammatic real-time approach, we develop a computer algorithm that generates and calculates all transport contributions up to second order in the tunnel-coupling strengths for arbitrary multi-island systems. This comprises sequential and cotunneling, as well as terms corresponding to a renormalization of charging energies and tunneling conductances. Multi-island cotunneling processes with energy transfer between different island are taken into account. To illustrate our approach we analyze the current through an island in Coulomb blockade, that is electrostatically coupled to a second island through which a large current is flowing. In this regime both cotunneling processes involving one island only as well as multi-island processes are important. The latter can be understood as photon-assisted sequential tunneling in the blockaded island, where the photons are provided by potential fluctuations due to sequential tunneling in the second island. We compare results of our approach to a P(E)-theory for photon-assisted tunneling in the weak coupling limit.Comment: 14 pages, 7 figures, published version; minor changes in Sec. IV

RELATIONSHIP BETWEEN ATOPIC DERMATITIS AND IMMUNOGLOBULIN E

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/66225/1/j.1365-4362.1976.tb00705.x.pd

Single-artificial-atom lasing using a voltage-biased superconducting charge qubit

We consider a system composed of a single artificial atom coupled to a cavity mode. The artificial atom is biased such that the most dominant relaxation process in the system takes the atom from its ground state to its excited state, thus ensuring population inversion. A recent experimental manifestation of this situation was achieved using a voltage-biased superconducting charge qubit. Even under the condition of `inverted relaxation', lasing action can be suppressed if the `relaxation' rate is larger than a certain threshold value. Using simple transition-rate arguments and a semiclassical calculation, we derive analytic expressions for the lasing suppression condition and the state of the cavity in both the lasing and suppressed-lasing regimes. The results of numerical calculations agree very well with the analytically derived results. We start by analyzing a simplified two-level-atom model, and we then analyze a three-level-atom model that should describe accurately the recently realized superconducting artificial-atom laser.Comment: 21 pages in preprint format, 6 figure

Formation of early-type galaxies from cosmological initial conditions

We describe high resolution Smoothed Particle Hydrodynamics (SPH) simulations of three approximately $M_*$ field galaxies starting from \LCDM initial conditions. The simulations are made intentionally simple, and include photoionization, cooling of the intergalactic medium, and star formation but not feedback from AGN or supernovae. All of the galaxies undergo an initial burst of star formation at $z \approx 5$, accompanied by the formation of a bubble of heated gas. Two out of three galaxies show early-type properties at present whereas only one of them experienced a major merger. Heating from shocks and -PdV work dominates over cooling so that for most of the gas the temperature is an increasing function of time. By $z \approx 1$ a significant fraction of the final stellar mass is in place and the spectral energy distribution resembles those of observed massive red galaxies. The galaxies have grown from $z=1 \to 0$ on average by 25% in mass and in size by gas poor (dry) stellar mergers. By the present day, the simulated galaxies are old ($\approx 10 {\rm Gyrs}$), kinematically hot stellar systems surrounded by hot gaseous haloes. Stars dominate the mass of the galaxies up to $\approx 4$ effective radii ($\approx 10$ kpc). Kinematic and most photometric properties are in good agreement with those of observed elliptical galaxies. The galaxy with a major merger develops a counter-rotating core. Our simulations show that realistic intermediate mass giant elliptical galaxies with plausible formation histories can be formed from \LCDM initial conditions even without requiring recent major mergers or feedback from supernovae or AGN.Comment: accepted for publication in Ap

Coherent and sequential photoassisted tunneling through a semiconductor double barrier structure

We have studied the problem of coherent and sequential tunneling through a double barrier structure, assisted by light considered to be present All over the structure, i,e emitter, well and collector as in the experimental evidence. By means of a canonical transformation and in the framework of the time dependent perturbation theory, we have calculated the transmission coefficient and the electronic resonant current. Our calculations have been compared with experimental results turning out to be in good agreement. Also the effect on the coherent tunneling of a magnetic field parallel to the current in the presence of light, has been considered.Comment: Revtex3.0, 8figures uuencoded compressed tar-fil

Internally Electrodynamic Particle Model: Its Experimental Basis and Its Predictions

The internally electrodynamic (IED) particle model was derived based on overall experimental observations, with the IED process itself being built directly on three experimental facts, a) electric charges present with all material particles, b) an accelerated charge generates electromagnetic waves according to Maxwell's equations and Planck energy equation and c) source motion produces Doppler effect. A set of well-known basic particle equations and properties become predictable based on first principles solutions for the IED process; several key solutions achieved are outlined, including the de Broglie phase wave, de Broglie relations, Schr\"odinger equation, mass, Einstein mass-energy relation, Newton's law of gravity, single particle self interference, and electromagnetic radiation and absorption; these equations and properties have long been broadly experimentally validated or demonstrated. A specific solution also predicts the Doebner-Goldin equation which emerges to represent a form of long-sought quantum wave equation including gravity. A critical review of the key experiments is given which suggests that the IED process underlies the basic particle equations and properties not just sufficiently but also necessarily.Comment: Presentation at the 27th Int Colloq on Group Theo Meth in Phys, 200

Translationally invariant nonlinear Schrodinger lattices

Persistence of stationary and traveling single-humped localized solutions in the spatial discretizations of the nonlinear Schrodinger (NLS) equation is addressed. The discrete NLS equation with the most general cubic polynomial function is considered. Constraints on the nonlinear function are found from the condition that the second-order difference equation for stationary solutions can be reduced to the first-order difference map. The discrete NLS equation with such an exceptional nonlinear function is shown to have a conserved momentum but admits no standard Hamiltonian structure. It is proved that the reduction to the first-order difference map gives a sufficient condition for existence of translationally invariant single-humped stationary solutions and a necessary condition for existence of single-humped traveling solutions. Other constraints on the nonlinear function are found from the condition that the differential advance-delay equation for traveling solutions admits a reduction to an integrable normal form given by a third-order differential equation. This reduction also gives a necessary condition for existence of single-humped traveling solutions. The nonlinear function which admits both reductions defines a two-parameter family of discrete NLS equations which generalizes the integrable Ablowitz--Ladik lattice.Comment: 24 pages, 4 figure