86 research outputs found
Gene Expression Analysis of Forskolin Treated Basilar Papillae Identifies MicroRNA181a as a Mediator of Proliferation
Auditory hair cells spontaneously regenerate following injury in birds but not mammals. A better understanding of the molecular events underlying hair cell regeneration in birds may allow for identification and eventually manipulation of relevant pathways in mammals to stimulate regeneration and restore hearing in deaf patients.Gene expression was profiled in forskolin treated (i.e., proliferating) and quiescent control auditory epithelia of post-hatch chicks using an Affymetrix whole-genome chicken array after 24 (n = 6), 48 (n = 6), and 72 (n = 12) hours in culture. In the forskolin-treated epithelia there was significant (p<0.05; >two-fold change) upregulation of many genes thought to be relevant to cell cycle control and inner ear development. Gene set enrichment analysis was performed on the data and identified myriad microRNAs that are likely to be upregulated in the regenerating tissue, including microRNA181a (miR181a), which is known to mediate proliferation in other systems. Functional experiments showed that miR181a overexpression is sufficient to stimulate proliferation within the basilar papilla, as assayed by BrdU incorporation. Further, some of the newly produced cells express the early hair cell marker myosin VI, suggesting that miR181a transfection can result in the production of new hair cells.These studies have identified a single microRNA, miR181a, that can cause proliferation in the chicken auditory epithelium with production of new hair cells
Is it possible to diagnose benign positional paroxysmal vertigo from primary care referrals to ENT clinic?
A mouse model for benign paroxysmal positional vertigo with genetic predisposition for displaced otoconia
Long-Term follow-up after Gentamicin Application via the Silverstein MicroWick in the Treatment of Ménière's Disease
Positional Vertigo as a First Symptom of a Cerebellopontine Angle Cholesteatoma: Case Report
Assessment of Vestibular Schwannoma Growth: Application of a New Measuring Protocol to the Results of a Longitudinal Study
Responses of non-eye-movement central vestibular neurons to sinusoidal yaw rotation in compensated macaques after unilateral semicircular canal plugging
Posterior Semicircular Canal Occlusion for Intractable Benign Paroxysmal Positional Vertigo
Preservation of hearing in patients undergoing excision of acoustic neuromas and other cerebellopontine angle tumors
Facial nerve preservation in patients with large acoustic neuromas treated by a combined middle fossa transtentorial translabyrinthine approach
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