2 research outputs found
UVB radiation induced effects on cells studied by FTIR spectroscopy
We have made a preliminary analysis of the results about the eVects on
tumoral cell line (lymphoid T cell line Jurkat) induced by UVB radiation (dose
of 310 mJ/cm^2) with and without a vegetable mixture. In the present study, we
have used two techniques: Fourier transform infrared spectroscopy (FTIR) and
flow cytometry. FTIR spectroscopy has the potential to provide the
identiWcation of the vibrational modes of some of the major compounds (lipid,
proteins and nucleic acids) without being invasive in the biomaterials. The
second technique has allowed us to perform measurements of cytotoxicity and to
assess the percentage of apoptosis. We already studied the induction of
apoptotic process in the same cell line by UVB radiation; in particular, we
looked for correspondences and correlations between FTIR spetroscopy and flow
cytometry data finding three highly probable spectroscopic markers of apoptosis
(Pozzi et al. in Radiat Res 168:698-705, 2007). In the present work, the
results have shown significant changes in the absorbance and spectral pattern
in the wavenumber protein and nucleic acids regions after the treatments