Tissue-Specific Transcriptomics of the Exotic Invasive Insect Pest Emerald Ash Borer (Agrilus planipennis)

BACKGROUND: The insect midgut and fat body represent major tissue interfaces that deal with several important physiological functions including digestion, detoxification and immune response. The emerald ash borer (Agrilus planipennis), is an exotic invasive insect pest that has killed millions of ash trees (Fraxinus spp.) primarily in the Midwestern United States and Ontario, Canada. However, despite its high impact status little knowledge exists for A. planipennis at the molecular level. METHODOLOGY AND PRINCIPAL FINDINGS: Newer-generation Roche-454 pyrosequencing was used to obtain 126,185 reads for the midgut and 240,848 reads for the fat body, which were assembled into 25,173 and 37,661 high quality expressed sequence tags (ESTs) for the midgut and the fat body of A. planipennis larvae, respectively. Among these ESTs, 36% of the midgut and 38% of the fat body sequences showed similarity to proteins in the GenBank nr database. A high number of the midgut sequences contained chitin-binding peritrophin (248)and trypsin (98) domains; while the fat body sequences showed high occurrence of cytochrome P450s (85) and protein kinase (123) domains. Further, the midgut transcriptome of A. planipennis revealed putative microbial transcripts encoding for cell-wall degrading enzymes such as polygalacturonases and endoglucanases. A significant number of SNPs (137 in midgut and 347 in fat body) and microsatellite loci (317 in midgut and 571 in fat body) were predicted in the A. planipennis transcripts. An initial assessment of cytochrome P450s belonging to various CYP clades revealed distinct expression patterns at the tissue level. CONCLUSIONS AND SIGNIFICANCE: To our knowledge this study is one of the first to illuminate tissue-specific gene expression in an invasive insect of high ecological and economic consequence. These findings will lay the foundation for future gene expression and functional studies in A. planipennis

Inside and out: the activities of senescence in cancer.

The core aspect of the senescent phenotype is a stable state of cell cycle arrest. However, this is a disguise that conceals a highly active metabolic cell state with diverse functionality. Both the cell-autonomous and the non-cell-autonomous activities of senescent cells create spatiotemporally dynamic and context-dependent tissue reactions. For example, the senescence-associated secretory phenotype (SASP) provokes not only tumour-suppressive but also tumour-promoting responses. Senescence is now increasingly considered to be an integrated and widespread component that is potentially important for tumour development, tumour suppression and the response to therapy.This is the author accepted manuscript. The final version is available from NPG via http://dx.doi.org/10.1038/nrc377

Late Miocene valley-confined subglacial volcanism in northern Alexander Island, Antarctic Peninsula

Isolated, Late Miocene volcanogenic sequences in northern Alexander Island, Antarctic Peninsula, form an unusual, cogenetic association of volcaniclastic, sandy-gravelly lithofacies (including tillites) and volcanic (lava/hyalocalstite) lithofacies. Using simple lithofacies analysis and theoretical considerations of hydrodynamic effects of subglacial eruptions, valley-confined volcanic activity beneth thin, wet-based ice is suggested. The Alexander Island successions are complete enough to be regarded as model sequences for this uncommonly recorded type of eruptive/depositional activity. The sedimentary lithofacies represent resedimented tuffs and meltout or flow tills, which were probably deposited in subglacial ice tunnels eroded or enlarged by volcanically heated meltwater. The volcanic lithofacies formed by the interaction of hot magma with the ice tunnel walls (generating abundant meltwater) and water-saturatedsediments, resulting in the formation of heterogeneous masses of lava and hyaloclastite. There is no obvious sequence organisation in the sedimentary sections. This is probably due to a complex interplay of eruption-related and environmental hydrodynamic factors affecting the relative proportions of water and entrained sediment