10 research outputs found
Structure determination of the disialylated poly-(N-acetyllactosamine)-containingO-linked carbohydrate chains of equine chorionic gonadotropin
No full textPrimary structure of the N-linked carbohydrate chains of calreticulin from spinach leaves
No full textCalreticulin is a multifunctional Ca2+-binding protein of the endoplasmic reticulum of most eukaryotic cells. The 56 kDa Calreticulin glycoprotein isolated from spinach (Spinacia oleracea L.) leaves was N-deglycosylated by PNGase-F digestion. The carbohydrate moiety was isolated by gel permeation chromatography and purified by high-pH anion-exchange chromatography. The fractions were investigated by 500 MHz H-1-NMR spectroscopy, in combination with monosaccharide analysis and fast-atom bombardment-mass spectrometry. The following carbohydrate structure could be established as the major component (Man(8)GlcNAc(2)):
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Heterogeneity was demonstrated by the presence of two minor components being Man(7)GlcNAc(2) lacking a terminal residue (D-1 or D-3), compared to the major component. A. cross-reactivity with an antibody against the endoplasmic reticulum retention signal HDEL was also found
Primary structure of the N-linked carbohydrate chains of Calreticulin from spinach leaves
No full textAction of rat liver Galβ1-4GlcNAc α(2-6)-sialyltransferase on Manβ1-4GlcNAcβ-OMe, GalNAcβ1-4GlcNAcβ-OMe, Glcβ1-4GlcNAcβ-OMe and GlcNAcβ1-4GlcNAcβ-OMe as synthetic substrates
No full textDifferent oligosaccharides accumulate in the brain and urine of a cat with ?-mannosidosis: structure determination of five brain-derived and seventeen urinary oligosaccharides
No full textEnzymatic characterization of CMP-NeuAc:Galβ1-4GlcNAc-R α(2-3)-sialyltransferase from human placenta
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