Removing orientation-induced localization biases in single-molecule microscopy using a broadband metasurface mask

Nanoscale localization of single molecules is a crucial function in several advanced microscopy techniques, including single-molecule tracking and wide-field super-resolution imaging. Until now, a central consideration of such techniques is how to optimize the precision of molecular localization. However, as these methods continue to push towards the nanometre size scale, an increasingly important concern is the localization accuracy. In particular, single fluorescent molecules emit with an anisotropic radiation pattern of an oscillating electric dipole, which can cause significant localization biases using common estimators. Here we present the theory and experimental demonstration of a solution to this problem based on azimuthal filtering in the Fourier plane of the microscope. We do so using a high-efficiency dielectric metasurface polarization/phase device composed of nanoposts with subwavelength spacing. The method is demonstrated both on fluorophores embedded in a polymer matrix and in dL5 protein complexes that bind malachite green

Experimental observation of a polarization vortex at an optical bound state in the continuum

Optical bound states in the continuum (BICs) are states supported by a photonic structure that are compatible with free-space radiation, yet become perfectly bound for one specific in-plane momentum and wavelength. Recently, it was predicted that light radiated by such modes around the BIC momentum–frequency condition should display a vortex in its far-field polarization profile, making the BIC topologically protected. Here, we study a one-dimensional grating supporting a transverse magnetic mode with a BIC near 700 nm wavelength, verifying the existence of the BIC using reflection measurements, which show a vanishing reflection feature. Using k-space polarimetry, we measure the full polarization state of reflection around the BIC, highlighting the presence of a topological vortex. We use an electromagnetic dipole model to explain the observed BIC through destructive interference between two radiation channels, characteristic of a Friedrich–Wintgen-type BIC. Our findings shed light on the origin of BICs and verify their topological nature