18 research outputs found
Geographical markers for Saccharomyces cerevisiae strains with similar technological origin domesticated for rice wine production in Northeastern states of India
Autochthonous strains of Saccharomyces
cerevisiae from traditional starters used for the
production of rice-based ethnic fermented beverage
in North East India were examined for their genetic
polymorphism using mitochondrial DNA-RFLP and
electrophoretic karyotyping. Mitochondrial DNARFLP
analysis of S. cerevisiae strains with similar
technological origins from hamei starter of Manipur
and marcha starter of Sikkim revealed widely
separated clusters based on their geographical origin.
Electrophoretic karyotyping showed high polymorphism
amongst the hamei strains within similar
mitochondrial DNA-RFLP cluster and one unique
karyotype of marcha strain was widely distributed in
the Sikkim-Himalayan region. We conceptualized the
possibility of separate domestication events for hamei
strains in Manipur (located in the Indo-Burma
biodiversity hotspot) and marcha strains in Sikkim
(located in Himalayan biodiversity hotspot), as a
consequence of less homogeneity in the genomic
structure between these two groups, their clear
separation being based on geographical origin, but
not on technological origin and low strain level
diversity within each group. The molecular markers
developed based on HinfI-mtDNA-RFLP profile and
the chromosomal doublets in chromosome VIII
position of Sikkim-Himalayan strains could be
effectively used as geographical markers for authenticating
the above starter strains and differentiating
them from other commercial strains