Características histoquímicas del músculo esquelético en caballos andaluces: estudio comparado con otras razas

The purpose of this study was to characterize the histochemical and morphometric profiles of the skeletal muscle in adult Andalusian horses, and compare it with those of Thoroughbred and Arabian horses. Muscle biopsies were taken from the Gluteus medius of 32 Andalusian horses (15 mares, 17 stallions), 24 Thoroughbred (17 mares, 7 stallions), and 15 Arabians (7 mares, 8 stallions), ranging from 5 to 24 years. Consistent sex differences in fibre type composition and fibre area were observed. A highly significant difference among breeds was also obtained, probably being related to the type of work for which the breeds have been selected. Results also imply that histochemical and morphometric properties of the skeletal muscle in Andalusians provide only a moderate endurance potential. However, since genetic factors are of importance, the endurance potential of a breed could be significantly increased by means of a correct genetic program.El presente estudio trata de caracterizar los perfiles histoquímico y morfométrico del mús- culo esquelético en caballos Andaluces adultos, y compararlos con los de caballos Purasangre y Arabes. Se obtuvieron biopsias del músculo Gluteus medius de 32 caballos Andaluces (15 yeguas, 17 sementales), 24 Purasangre (17 yeguas, 7 sementales), y 15 Arabes (7 yeguas, 8 sementales), de 5 a 24 años de edad. Se observaron importantes diferencias en el porcentaje y tamaño de las fibras entre sexos y también entre razas, según el tipo de trabajo para el que cada raza ha sido específicamente seleccionada. Las propiedades histoquímicas y morfométricas del músculo esquelético en el caballo Andaluz sólo le proporcionan una capacidad de resistencia moderada. No obstante, dada la importancia de los factores genéticos, el potencial de resistencia de los caballos Andaluces podría modificarse significativamente mediante un adecuado programa genético

Morphologic and morphometric study of the musculus obliquus dorsalis of the dog

In the present investigation, the dorsal oblique muscle of the right ocular globe was removed from six adult dogs weighing 40-50 kg and analyzed by light microscopy. Muscle samples were taken from the central portion of the muscle belly, subsequently ultrafrozen, cut and stained with m-ATPase at pH 4.6. Fibers were classified as type I or type II according to their reaction to the m-ATPase and detailed morphologic and morphometric studies were made. The muscles showed two clearly distinct layers, a central layer and a peripheral layer, mainly composed of type II fibers. The fibers in the central layer were larger in size than those in the peripheral layer. The peculiar stratigraphy of the dorsal oblique muscle should be taken into account when performing analyses of this muscle and investigating the significance of the fiber types it contains.En el presente trabajo se han extraído, de los globos oculares derechos, de seis perros adultos de 40-50 kilogramos de peso, los músculos oblicuos dorsales y analizados con microscopia de luz. Las muestras musculares para su análisis se tomaron de la porción central del vientre muscular y fueron ultracongeladas, cortadas y teñidas por la técnica de m-ATPasa a pH 4,6. Las fibras se clasificaron en tipo I y tipo II en virtud a su reacción frente a la m-ATPasa, realizándose un detallado estudio morfológico y morfométrico. Los músculos presentaron dos estratos netamente diferenciados, uno central y otro periférico, compuestos mayoritariamente por fibras tipo II. Las fibras del estrato central presentaron un mayor tamaño que las correspondientes del estrato periférico. La peculiar estratigrafía del músculo oblicuo dorsal debería tenerse en consideración al analizar este músculo e investigar el significado de los tipos fibrilares que contien

Cooling Strategies for Greenhouses in Summer: Control of Fogging by Pulse Width Modulation

The possibilities for improving the control of greenhouse fogging systems, were studied by comparing several combinations of ventilation cooling techniques, shade screening and low-pressure fogging. The study was divided into three parts: experiments, modelling and simulations. In the first part of the paper, ten combinations of five cooling techniques were tested during the summers of 2002 and 2003 in a 132m2 greenhouse with a steel structure and a single-layer methacrylate cover located in Madrid, Spain. An analysis of variance of the climatic parameters was carried out to determine which combinations produced significant differences in inside temperature or relative humidity. Comparing the values for the inside to outside temperature difference, the combination of a shade screen and above-screen fogging achieved a difference in temperature almost the same as that for under-screen fogging, but the relative humidity was significantly lower. In the second part of the study a dynamic model was developed (2002) and validated (2003). The mean absolute error obtained for inside temperature was similar in the fit and the validation and it was less than 1.5 1C in both cases. The model was used to simulate the inside air temperature for a fog system working without shading, and above and under a shade screen. Control algorithms were developed for reducing system water consumption. In the three cases a simple on/off control with a fixed fogging cycle was compared with a pulse width modulation (PWM) strategy, in which the duration of the fogging pulse was increased as a function of inside temperature. The strategies with PWM applied to the fog system were able to reduce water consumption by 8–15% with respect to the strategies with a fixed fogging cycle

Morphometric study of the nerve fibers of the oculomotor nerve in dog

The intracranial portion of the right oculomotor nerves was extracted from six adult German Shepherd dogs. The nerves were studied using light and electron microscopy. Nerve area was calculated, and myelinated and unmyelinated fibers were analized to determine number, diameter and area. Also in the myelinated fibers the area and diameter of the correspondent axon, and thickness of the myeline sheath were measured. The mean number of fibers was 8543.50±1231.85 for myelinated and 1402.00±241.58 for unmyelinated. The mean fiber diameter was 10.23±0.68 μm for myelinated and 0.43±0.21 μm for unmyelinated. This study demonstrated that the oculomotor nerves in dogs have structural and ultrastructural characteristics that are similar to those from other specie

Stoichlometry control over a wide composition range of sputtered CuGa_(x)In_(1-x)Se_(2)

Films of CuGaxIn(1-x)Se2 (CGIS) have been grown by rf sputtering from stoichiometric single targets with different Ga/In ratios. Adjusting growth temperature and argon pressure we are able to deposit films with a wide range of Cu contents: From CGIS Cu-poor (16 at. %) to Cu2Se. Reevaporation of (Ga,In)2Se3 binaries is observed when substrate temperature is increased at a constant argon pressure (20 mTorr). An increase in Ar pressure from 5 to 150 mTorr at a growth temperature of 450-degrees-C, produces a decrease in Cu atomic percentage from 24% to 16% due to a preferential diffusion of Cu sputtered atoms in the plasma. The relevant film properties of the analyzed films are found to be ruled by the Cu content. Graded composition absorbers with adequate physical properties for the fabrication of photovoltaic devices are grown with a proper choice of growth parameters

Arsenic exposure, diabetes-related genes and diabetes prevalence in a general population from Spain

Inorganic arsenic exposure may be associated with diabetes, but the evidence at low-moderate levels is not sufficient. Polymorphisms in diabetes-related genes have been involved in diabetes risk. We evaluated the association of inorganic arsenic exposure on diabetes in the Hortega Study, a representative sample of a general population from Valladolid, Spain. Total urine arsenic was measured in 1451 adults. Urine arsenic speciation was available in 295 randomly selected participants. To account for the confounding introduced by non-toxic seafood arsenicals, we designed a multiple imputation model to predict the missing arsenobetaine levels. The prevalence of diabetes was 8.3%. The geometric mean of total arsenic was 66.0 µg/g. The adjusted odds ratios (95% confidence interval) for diabetes comparing the highest with the lowest tertile of total arsenic were 1.76 (1.01, 3.09) and 2.14 (1.47, 3.11) before and after arsenobetaine adjustment, respectively. Polymorphisms in several genes including IL8RA, TXN, NR3C2, COX5A and GCLC showed suggestive differential associations of urine total arsenic with diabetes. The findings support the role of arsenic on diabetes and the importance of controlling for seafood arsenicals in populations with high seafood intake. Suggestive arsenic-gene interactions require confirmation in larger studies

El Perfil sísmico ALCUDIA: una imagen de la Zona Centroibérica (Varisco Ibérico meridional, España)

The 250 km long, vertical incidence seismic reflection profile ALCUDIA was acquired in spring 2007. It samples the Variscan Central Iberian Zone from Toledo to Fuenteovejuna. Its main goal was to continue the structure obtained for the crust and mantle from the IBERSEIS transect towards the N and NE. The acquisition parameters, similar to those used in the IBERSEIS profile have proven to be adequate to show a detailed image of the whole crust and upper mantle. After preliminary processing, the upper crust shows a moderate reflectivity that can be easily correlated with identifiable surface geologic features. The middle and lower part of the crust seems to be very reflective, laminated and afected by deformation, although to a different extent. The Moho is placed at approximately 10 s TWT and appears to be flat in the time section. The section can be divided into four domains of reflectivity that can be key to stablish different domains of crustal evolution. This new transect, together with the previous IBERSEIS profile, complete an almost 600 km long lithospheric section that crosses the southern half of the Iberian Variscides.El perfil sísmico de reflexión ALCUDIA , de 250 km de longitud, fue adquirido en la primavera del año 2007. Este perfil ha muestreado la Zona Centroibérica entre Toledo y Fuenteovejuna, y su objetivo principal ha sido continuar hacia el NE la estructura de corteza y manto ya obtenida mediante el perfil IBERSEIS. Los parámetros de adquisición, similares a los de IBERSEIS, han sido adecuados para mostrar una imagen detallada de toda la corteza y el manto superior. Una vez efectuado el procesado preliminar, la corteza superior presenta una reflectividad moderada que puede correlacionarse fácilmente con rasgos geológicos de superficie. Las partes media e inferior de la corteza son muy reflectivas, laminadas y afectadas por una deformación que varía a lo largo del perfil. La Moho se localiza a unos 10 s TWT y tiene geometría plana en la sección de tiempo. La variación en los patrones generales de reflectividad permite dividir el pefil sísmico en cuatro dominios, que corresponden a diferentes evoluciones corticales. Este nuevo perfil sísmico, unido al anterior perfil IBERSEIS, constituye una sección litosférica de casi 600 km de longitud, que atraviesa la parte meridional del Varisco Ibérico.Depto. de Geodinámica, Estratigrafía y PaleontologíaFac. de Ciencias GeológicasTRUEMinisterio de Ciencia e Innovación (MICINN)Generalitat de CatalunyaJunta de Comunidades de Castilla La Mancha.pu

The Quest for Orthologs benchmark service and consensus calls in 2020.

The identification of orthologs-genes in different species which descended from the same gene in their last common ancestor-is a prerequisite for many analyses in comparative genomics and molecular evolution. Numerous algorithms and resources have been conceived to address this problem, but benchmarking and interpreting them is fraught with difficulties (need to compare them on a common input dataset, absence of ground truth, computational cost of calling orthologs). To address this, the Quest for Orthologs consortium maintains a reference set of proteomes and provides a web server for continuous orthology benchmarking (http://orthology.benchmarkservice.org). Furthermore, consensus ortholog calls derived from public benchmark submissions are provided on the Alliance of Genome Resources website, the joint portal of NIH-funded model organism databases

Health providers as bricoleurs:an examination of the adaption of health ecosystems to superdiversity in Europe

This article examines the ways in which healthcare providers from a mixed economy of welfare operating in superdiverse neighbourhoods connect and innovate across the healthcare ecosystem to meet diverse and complex needs. Moving beyond a health systems approach which siloes different types of provision, we use the concept of bricolage to make visible the work undertaken by providers across the ecosystem. While we show that public, private and civil society provision all adapt to meet complex and diverse needs to some degree, we highlight the importance of inter-connectedness between providers and note the role of civil society in addressing gaps and cracks in provision. The importance of adopting a whole ecosystem approach and focusing on the actions and interactions which enable the ecosystem to function in complex demographic environments is highlighted before we stress the dangers of over-reliance on civil society.info:eu-repo/semantics/acceptedVersio