Mechanical modulation of a human plasma based skin scaffold via reactive multi-arm polyethylene glycols

Accésit Congreso SIBB 2016Equivalentes dermo-epidérmicos basados en plasma humano autólogo han sido diseñados a lo largo de la última década con el objetivo de tratar quemaduras y heridas quirúrgicas; aun así, las malas propiedades mecánicas como son la fragilidad o la contracción durante el cultivo y la implantación persisten y requieren soluciones.  Este artículo investiga la modulación mecánica de equivalentes dermo-epidérmicos basados en plasma humano mediante la introducción de una red tridimensional (3D) polimérica interpenetrante y biodegradable compuesta por polietilenglicol tetra-glutarato de succinimidil (4SG-PEG) y polietilenglicol tetra-amino (4A-PEG), con pesos moleculares de 10kDa. Las propiedades físicas de estos hidrogeles de fibrina y PEG fueron caracterizadas mediante la cuantificación de grupos aminos libres, el tiempo de gelificación, el hinchamiento de forma temporal y la liberación de proteínas. Además, la viabilidad de fibroblastos y queratinocitos primarios humanos fueron estudiadas mediante los ensayos Alamar Blue y MTS respectivamente. Los resultados obtenidos sugieren que mediante la introducción de una red interpenetrante basada en PEG y fibrina, las propiedades mecánicas de los geles de fibrina fueron moduladas positivamente, sin disminuir significativamente la viabilidad celular. Estos resultados prometen futuras investigaciones para mejorar las propiedades mecánicas de estos equivalentes dermo-epidérmicos.Peer ReviewedAward-winnin

Perturbacion local de la estabilidad estructural del DNA por discontinuidades de cadena

Centro de Informacion y Documentacion Cientifica (CINDOC). C/Joaquin Costa, 22. 28002 Madrid. SPAIN / CINDOC - Centro de Informaciòn y Documentaciòn CientìficaSIGLEESSpai

Analisis por sedimentacion de heterogeneidad macromolecular en DNA

Centro de Informacion y Documentacion Cientifica (CINDOC). C/Joaquin Costa, 22. 28002 Madrid. SPAIN / CINDOC - Centro de Informaciòn y Documentaciòn CientìficaSIGLEESSpai

Abnormal cochlea linked to deafness in transgenic mice expressing human cytokeratin K8

The cytokeratin intermediate filaments have a relevant role in the proliferation and differentiation processes of epithelial cells. To provide information about the role of K8 cytokeratin during the auditory receptor differentiation, two groups of adult mice were used: TGK8-4 transgenic and control animals. The TGK8-4 transgenic mice contained 12 kb of K8 human cytokeratin (HK8) locus (Casanova et al., 1995, 1999). The functional activity of the auditory receptor was analyzed by auditory thresholds. Morphological studies demonstrate that the auditory receptors of the TGK8-4 transgenic mice are highly immature. Immunocytochemical studies were made by using two monoclonal antibodies: CAM 5-2 (recognizing K8 human cytokeratin) and Troma-1 (recognizing both mouse and human K8 cytokeratin). These demonstrated significant differences between the auditory receptors of the transgenic mice and the control mice. These functional and morphological differences clearly suggest that K8 cytokeratin has a relevant role during the differentiation and tridimensional organization of the sensory and the supporting cells of the auditory receptor

The role of versican in the skin ECM and its interaction with hyaluronic acid

Premio Accésit Congreso SIBB 2019The extracellular matrix (ECM) is a structural network that comprises the bulk of the tissues. It acts as a supporting scaffold for the cells to develop their function and plays an active role in many processes such as proliferation and migration. Therefore, the ECM is an interesting object of study for regenerative medicine. In this article we make an extensive review of two key components of the ECM: the glycosaminoglycan Hyaluronic Acid (HA) and the proteoglycan Versican (Ver). These two molecules are present in the skin ECM and play active roles in processes such as differentiation, wound healing, hair follicle cycle and development. Award-winningPremio Accésit Congreso SIBB 2019Peer Reviewe

Skin gene therapy for acquired and inherited disorders

The rapid advances associated with the Human Genome Project combined with the development of proteomics technology set the bases to face the challenge of human gene therapy. Different strategies must be evaluated based on the genetic defect to be corrected. Therefore, the re-expression of the normal counterpart should be sufficient to reverse phenotype in single-gene inherited disorders. A growing number of candidate diseases are being evaluated since the ADA deficiency was selected for the first approved human gene therapy trial (Blaese et al., 1995). To cite some of them: sickle cell anemia, hemophilia, inherited immune deficiencies, hyper-cholesterolemia and cystic fibrosis. The approach does not seem to be so straightforward when a polygenic disorder is going to be treated. Many human traits like diabetes, hypertension, inflammatory diseases and cancer, appear to be due to the combined action of several genes and environment. For instance, several wizard gene therapy strategies have recently been proposed for cancer treatment, including the stimulation of the immune system of the patient (Xue et al., 2005), the targeting of particular signalling pathways to selectively kill cancer cells (Westphal and Melchner, 2002) and the modulation of the interactions with the stroma and the vasculature (Liotta, 2001; Liotta and Kohn, 2001)