Hydrogeophysical imaging of deposit heterogeneity and groundwater chemistry changes during DNAPL source zone bioremediation

Robust characterization and monitoring of dense nonaqueous phase liquid (DNAPL) source zones is essential for designing effective remediation strategies, and for assessing the efficacy of treatment. In this study high-resolution cross-hole electrical resistivity tomography (ERT) was evaluated as a means of monitoring a field-scale in-situ bioremediation experiment, in which emulsified vegetable oil (EVO) electron donor was injected into a trichloroethene source zone. Baseline ERT scans delineated the geometry of the interface between the contaminated alluvial aquifer and the underlying mudstone bedrock, and also the extent of drilling-induced physical heterogeneity. Time-lapse ERT images revealed major preferential flow pathways in the source and plume zones, which were corroborated by multiple lines of evidence, including geochemical monitoring and hydraulic testing using high density multilevel sampler arrays within the geophysical imaging planes. These pathways were shown to control the spatial distribution of the injected EVO, and a bicarbonate buffer introduced into the cell for pH control. Resistivity signatures were observed within the preferential flow pathways that were consistent with elevated chloride levels, providing tentative evidence from ERT of the biodegradation of chlorinated solvents

River terrace sand and gravel deposit reserve estimation using three-dimensional electrical resistivity tomography for bedrock surface detection

We describe the application of 3D electrical resistivity tomography (ERT) to the characterisation and reserve estimation of an economic fluvial sand and gravel deposit. Due to the smoothness constraints used to regularise the inversion, it can be difficult to accurately determine the geometry of sharp interfaces. We have therefore considered two approaches to interface detection that we have applied to the 3D ERT results in an attempt to provide an accurate and objective assessment of the bedrock surface elevation. The first is a gradient-based approach, in which the steepest gradient of the vertical resistivity profile is assumed to correspond to the elevation of the mineral/bedrock interface. The second method uses an intrusive sample point to identify the interface resistivity at a location within the model, from which an iso-resistivity surface is identified that is assumed to define the interface. Validation of these methods has been achieved through direct comparison with observed bedrock surface elevations that were measured using real-time-kinematic GPS subsequent to the 3D ERT survey when quarrying exposed the bedrock surface. The gradient-based edge detector severely underestimated the depth to bedrock in this case, whereas the interface resistivity method produced bedrock surface elevations that were in close agreement with the GPS-derived surface. The failure of the gradient-based method is attributed to insufficient model sensitivity in the region of the bedrock surface, whereas the success of the interface resistivity method is a consequence of the homogeneity of the mineral and bedrock, resulting in a consistent interface resistivity. These results highlight the need for some intrusive data for model validation and for edge detection approaches to be chosen on the basis of local geological conditions

Effect of Invivo Administration of An Antibody to Epidermal Growth-Factor On the Rapid Increase in Dna-Synthesis Induced by Partial-Hepatectomy in the Rat

Recent reports indicate that transforming growth factor alpha (TGF-alpha) is produced within the fiver and acts as the natural ligand of the epidermal growth factor (EGF) receptor causing the EGF receptor down regulation and the hepatocyte proliferation observed after partial hepatectomy. The reported phenomenon that an antibody to EGF inhibits the regenerative response to partial hepatectomy was therefore re-investigated. The IgG fraction of an anti-rat EGF antibody was injected intravenously at the time of partial hepatectomy, and its effects on regenerative DNA synthesis were compared with those of non-immune IgG. Injection of IgG reduced the DNA synthetic response to partial hepatectomy, assessed 24 hours after resection by H-3-thymidine incorporation, but the effects of normal and anti-EGF IgG were not statistically different, despite the presence of excess anti-EGF IgG in the circulation throughout the experimental period. However, anti-EGF IgG could completely block the proliferative response of hepatocytes in culture to EGF. These results support the suggestion that EGF is not the major mediator of hepatocyte DNA synthesis in the early stages of liver regeneration