Tyrosine phosphorylation enrichment and subsequent analysis by MALDI-TOF/TOF MS/MS and LC-ESI-IT-MS/MS

Published Online: 1 NOV 2010This unit describes methods to detect, identify, and characterize tyrosine phosphorylation in proteins by mass spectrometry, including sample preparation methods, enrichment strategies using phosphotyrosine-specific antibodies, and chromatographic separation methods.Mark R. Condina, Manuela Klingler-Hoffmann and Peter Hoffman