Genetic variation and parental performance under inbreeding for growth in Eucalyptus globulus

We aimed to better understand the genetic architecture of growth in E. globulus undergoing inbreeding by comparing families from selfing (SELF), open pollination (OP) and unrelated polymix crossing (POL) of common parents. Stem diameter at breast height (DBH) was assessed at 4, 6 and 10 years after planting in a field trial. • The OP heritability was overestimated at an early age relative to the POL heritability. • No significant correlations were found between the SELF and POL parental effects, indicating substantial non-additive genetic variation under inbreeding. The OP family effects were better correlated with the SELF than the POL population, and only at age 10 years, after substantial mortality of inbred progeny has occurred, was the positive correlation between OP and POL families significantly different from zero. • The estimated dominance variance arising from inbreeding was nearly 10-fold greater than the dominance variance associated with random mating and the additive variance, and appeared to be a major contributor to the variation in inbreeding depression amongst selfed families

Effects of inbreeding on population mean performance and observational variances in Eucalyptus globulus

Mean performance and variances were studied in self (SELF), open pollinated (OP) and unrelated polymix (POL) crosses of common parentage in Eucalyptus globulus. • Inbreeding depression for survival (SURV) and basal area per hectare (BAH) was the highest reported for a SELF eucalypt population, increasing with age to reach 74 and 77%, respectively, over 10 years. • Inbreeding depression in the OP was 36% for SURV and 32% for BAH at age 10 years, and estimates of outcrossing rate from BAH were stable across ages, averaging 0.56. In contrast, OP inbreeding depression for stem diameter (DBH) of survivors decreased with age and few selfs appeared to survive to 10 years. • There was more variation in DBH between and within SELF than POL families, with variance ratios consistent with rare and partially recessive deleterious alleles causing inbreeding depression. • The OP variances were initially more similar to the SELF population but converged to the POL population after 10 years. • It is argued that when outcrossing rates are low, as in the present case, inbreeding depression will be a significant force countering local adaptation in forest trees