Transparent soil for imaging the rhizosphere

Understanding of soil processes is essential for addressing the global issues of food security, disease transmission and climate change. However, techniques for observing soil biology are lacking. We present a heterogeneous, porous, transparent substrate for in situ 3D imaging of living plants and root-associated microorganisms using particles of the transparent polymer, Nafion, and a solution with matching optical properties. Minerals and fluorescent dyes were adsorbed onto the Nafion particles for nutrient supply and imaging of pore size and geometry. Plant growth in transparent soil was similar to that in soil. We imaged colonization of lettuce roots by the human bacterial pathogen Escherichia coli O157:H7 showing micro-colony development. Micro-colonies may contribute to bacterial survival in soil. Transparent soil has applications in root biology, crop genetics and soil microbiology

Nutrition for master athletes: From challenges to optimisation strategies

Master athletes are genuine examples of successful ageing thanks to their capacity of maintaining a high level of sports performance during their entire life. Within the last decade many studies have focused on the evolution of performance in many sports with ageing, as well as on the training modalities to reach such performance. On the contrary, there is a paucity of data on the nutritional habits of master athletes and the question of whether they need to adapt their nutrition to the ageing process remains unsolved. This is within this context of the optimisation of nutrition for master athletes that this review stands. The aim is to identify the potential nutritional challenges encountered by master athletes and how to handle them through adapted nutritional strategies. Given the lack of data specific to master athletes, studies including master athletes are considered as a priority, though we also make inferences based on nutrition for young adults and perturbations induced by ageing in non-athletic populations. The first part of this opinion paper tackles the first challenge faced by master athletes which is the gradual reduction in energy intake with ageing. The second challenge is the anabolic resistance and the need for increased protein intake. The third nutritional challenge we present is the episodes of metabolic crisis master athletes may face throughout life. Finally, we gather the main nutritional recommendations for master athletes and suggest the next stage of research. © 2019 ACAPS

Nutrition for master athletes: is there a need for specific recommendations?

Master athletes are often considered exemplars of successful aging, thanks to their capacity to maintain a high sports performance during their entire life. A high training capacity, regular participation in sporting competitions, and delayed alterations in body composition and physiological capacities have been listed among the main factors contributing to impressive master athletes’ performances. However, there is a paucity of data on the metabolism and dietary habits of master athletes, and the question of whether they need to adapt their nutrition to the aging process remains open. Herein, the authors presented a contemporary overview of themetabolic challenges associated with aging, including the risk of low energy availability, anabolic resistance, and periods of metabolic crisis due to forced immobilization. After assembling scientific evidence to show that master athletes must adapt their dietary intake, the authors proposed a summary of nutritional recommendations for master athletes and suggested the next stage of research

Estimation of the asymptotic variance of semiparametric maximum likelihood estimators in the Cox model with a missing time-dependent covariate

ABSTRACT The relationship between a time-to-event and a time-dependent or time-independent covariate is usually assessed using the Cox model. A frequently encountered problem however is occurrence of missing covariate values. A recent approach for estimating the Cox model with a missing covariate jointly models the time-to-event and covariate. In the case of a time-dependent covariate, Dupuy and Mesbah [Dupuy, J.-F., have proposed a joint model and have obtained a semiparametric maximum likelihood estimator of the regression parameter of the Cox model that is consistent and asymptotically normal. Furthermore, an explicit expression was obtained for the asymptotic variance of this estimator. In this paper, we examine the problem of estimating this variance. We propose a computationally simple estimator and we show its consistency. We illustrate the approach by applications to real data sets

Transparent soil microcosms allow 3D spatial quantification of soil microbiological processes in vivo

The recently developed transparent soil consists of particles of Nafion, a polymer with a low refractive index (RI), which is prepared by milling and chemical treatment for use as a soil analogue. After the addition of a RI-matched solution, confocal imaging can be carried out in vivo and without destructive sampling. In a previous study, we showed that the new substrate provides a good approximation of plant growth conditions found in natural soils. In this paper, we present further development of the techniques for detailed quantitative analysis of images of root-microbe interactions in situ. Using this system it was possible for the first time to analyse bacterial distribution along the roots and in the bulk substrate in vivo. These findings indicate that the coupling of transparent soil with light microscopy is an important advance towards the discovery of the mechanisms of microbial colonisation of the rhizosphere

Biochemical characteristics and bacterial community structure of the sea surface microlayer in the South Pacific Ocean

The chemical and biological characteristics of the surface microlayer were determined during a transect across the South Pacific Ocean in October-December 2004. Concentrations of particulate organic carbon (1.3 to 7.6-fold) and nitrogen (1.4 to 7-fold), and POC:PON ratios were consistently higher in the surface microlayer as compared to surface waters (5 m). The large variability in particulate organic matter enrichment was negatively correlated to wind speed. No enhanced concentrations of dissolved organic carbon were detectable in the surface microlayer as compared to 5 m, but chromophoric dissolved organic matter was markedly enriched (by 2 to 4-fold) at all sites. Based on pigment analysis and cell counts, no consistent enrichment of any of the major components of the autotrophic and heterotrophic microbial community was detectable. CE-SSCP fingerprints and CARD FISH revealed that the bacterial communities present in the surface microlayer had close similarity (>76%) to those in surface waters. By contrast, bacterial heterotrophic production (<sup>3</sup>H-leucine incorporation) was consistently lower in the surface microlayer than in surface waters. By applying CARD-FISH and microautoradiography, we observed that <i>Bacteroidetes</i> and <i>Gammaproteobacteria</i> dominated leucine uptake in the surface microlayer, while in surface waters <i>Bacteroidetes</i> and <i>Alphaproteobacteria</i> were the major groups accounting for leucine incorporation. Our results demonstrate that the microbial community in the surface microlayer closely resembles that of the surface waters of the open ocean. Even a short residence in the surface microlayer influences leucine incorporation by different bacterial groups, probably as a response to the differences in the physical and chemical nature of the two layers

Mapping the Shores of the Brown Dwarf Desert III: Young Moving Groups

We present the results of an aperture masking interferometry survey for substellar companions around 67 members of the young (~8-200Myr) nearby (~5-86pc) AB Doradus, Beta Pictoris, Hercules-Lyra, TW Hya, and Tucana-Horologium stellar associations. Observations were made at near infrared wavelengths between 1.2-3.8 microns using the adaptive optics facilities of the Keck II, VLT UT4, and Palomar Hale Telescopes. Typical contrast ratios of ~100-200 were achieved at angular separations between ~40-320mas, with our survey being 100% complete for companions with masses below 0.25\msolar across this range. We report the discovery of a $0.52 \pm 0.09$\msolar companion to HIP14807, as well as the detections and orbits of previously known stellar companions to HD16760, HD113449, and HD160934. We show that the companion to HD16760 is in a face-on orbit, resulting in an upward revision of its mass from $M_2 \sin i \sim 14$\mjupiter to $M_2 = 0.28 \pm 0.04$\msolar. No substellar companions were detected around any of our sample members, despite our ability to detect companions with masses below 80\mjupiter for 50 of our targets: of these, our sensitivity extended down to 40\mjupiter around 30 targets, with a subset of 22 subject to the still more stringent limit of 20\mjupiter. A statistical analysis of our non-detection of substellar companions allows us to place constraints on their frequency around ~0.2-1.5\msolar stars. In particular, considering companion mass distributions that have been proposed in the literature, we obtain an upper limit estimate of ~9-11% for the frequency of 20-80\mjupiter companions between 3-30AU at 95% confidence, assuming that their semimajor axes are distributed according to $d\mathcal{N}/da \propto a^{-1}$ in this range.Comment: Accepted by Ap

A focus on L dwarfs with trigonometric parallaxes

This is an author-created, un-copyedited version of an article published in Publications of the Astronomical Society of the Pacific. Under embargo until 14 May 2019. IOP Publishing Ltd is not responsible for any errors or omissions in this version of the manuscript or any version derived from it. The Version of Record is available online at https://doi.org/10.1088/1538-3873/aaacc5.We report new parallax measurements for ten L and early T type dwarfs, five of which have no previous published values, using observations over 3 years at the robotic Liverpool Telescope. The resulting parallaxes and proper motions have median errors of 2\,mas and 1.5\,mas/year respectively. Their space motions indicate they are all Galactic disk members. We combined this sample with other objects with astrometry from the Liverpool Telescope and with published literature astrometry to construct a sample of 260 L and early T type dwarfs with measured parallaxes, designated the Astrometry Sample. We study the kinematics of the Astrometry Sample, and derived a solar motion of $(U,V,W)_{\bigodot} = (7.9\pm1.7,13.2\pm1.2,7.2\pm1.0)$\,\kms~ with respect to the local standard of rest, in agreement with recent literature. We derive a kinematic age of 1.5-1.7\,Gyr for the Astrometry Sample assuming the age increases monotonically with the total velocity for a given disk sample. This kinematic age is less than half literature values for other low mass dwarf samples. We believe this difference arises for two reasons (1) the sample is mainly composed of mid to late L dwarfs which are expected to be relatively young and (2) the requirement that objects have a measured parallax biases the sample to the brighter examples which tend to be younger.Peer reviewedFinal Accepted Versio

Risk-based inspection as a cost-effective strategy to reduce human exposure to cysticerci of Taenia saginata in low-prevalence settings

Taenia saginata cysticercus is the larval stage of the zoonotic parasite Taenia saginata, with a life-cycle involving both cattle and humans. The public health impact is considered low. The current surveillance system, based on post-mortem inspection of carcasses has low sensitivity and leads to considerable economic burden. Therefore, in the interests of public health and food production efficiency, this study aims to explore the potential of risk-based and cost-effective meat inspection activities for the detection and control of T. saginata cysticercus in low prevalence settings