8 research outputs found
Utjecaj morfoloŔkog tipa stanica na ekspresiju bcl-2, Ki-67 i p-53 u uvealnim melanomima
Uveal melanomas are known to be less aggressive malignant tumors compared with melanoma of other localizations but the same morphological characteristics. We hypothesized that immunohistochemical determination of expression of the known proliferation markers bcl-2 and ki-67, and p-53 as a suppressor gene marker, could better explain the biological behavior of uveal melanoma vs. melanoma of other localizations. Thirty cases of primary uveal melanoma of two levels of invasion were tested retrospectively for the presence of bcl-2, Ki-67 and p-53 proteins with the indirect peroxidase-antiperoxidase immunoenzyme method and three step ABC/AP method. The intensity of reaction was assessed by semiquantiative method.Usprkos morfoloÅ”koj graÄi sliÄnoj drugim melanomima, poznato je da uvealni melanomi imaju daleko bolju prognozu od melanoma drugih lokalizacija. Upravo stoga pretpostavili smo da bi odreÄivanje ekspresije bcl-2, Ki-67 kao poznatih Äimbenika proliferacije, te p-53 proteina u stanicama uvealnih melanoma moglo protumaÄiti ovakvo bioloÅ”ko ponaÅ”anje uvealnih melanoma. U tu svrhu ispitano je retrogradno 30 primarnih uvealnih melanoma stadija pT2 i pT3 na prisutnost bcl-2, Ki-67 i p-53 proteina indirektnom metodom peroksidaze-anti-peroksidaze i trofaznom metodom ABC/AP. Stupanj reaktivnosti odreÄen je semikvantitativnom metodom
Lymphocytic Adenohypophysitis Simulating a Pituitary Adenoma in a Pregnant Woman
The lymphocytic hypophysitis, appearing in women during the third trimester of
pregnancy or early post-partum period, is a rare cause of hypopituitarism and pituitary
enlargement. A 39 year-old woman presented in the 37th week of pregnancy with bilateral
heteronymous quadrantanopsia, CT indicative of tumorous mass and symptoms of
hypopituitarism with decreased thyroid hormone and thyrotrophin levels, and low normal
level of cortisol. After the birth of a healthy male child the patient breastfed for 10
days, sight disturbances disappeared, but amenorrhea persisted. Upon admittance the
visual field showed no abnormalities. MR of the sellar region confirmed previous CT
findings. Endocrinological testing confirmed secondary hypothyroidism and cortisol
deficiency, normal levels of prolactin with satisfactory reaction to thyroliberin. Histology
showed mononuclear infiltration, and immunohistochemistry revealed T-cells (CD3)
at the borders, and B-cells (CD20) in the follicular center. Due to enlargement of the pituitary
associated with hypopituitarism, an incorrect diagnosis of a tumor could be mad
Nicotinamide Adenine Dinucleotide Phosphate-Diaphorase (NADPH-d) Histochemistry Detecting NOS in Healthy and Chronically Inflamed Pulp
The aim of this study was to examine the expression of nicotinamide adenine dinucleotide
phosphate-diaphorase (NADPH-d) activity in human dental pulps and determine
whether there are changes of the activity in chronically inflamed pulp tissue. Nineteen
pulps with clinical diagnosis of chronic pulpitis were collected during endodontic
treatment. The healthy controls were obtained from teeth extracted for orthodontic
therapy. The clinical diagnosis was confirmed by histological analysis. Healthy pulps
showed stratified odontoblasts in peripheral parts, while in central area there was normal
connective tissue. Chronically inflamed pulps showed less expressed stratification
of odontoblasts and infiltration of lymphocytes, polymorphonuclear leukocytes, plasma
cells and mastocytes. NADPH-d granular reactivity was assessed semi quantitatively
under the light microscope by a single observer and scored on an intensity scale from
negative reaction to very strong reaction. In healthy human pulps, NADPH-d activity
was strong to very strong in odontoblastic layer. Endothelial cells and Schwann cells
showed strong NADPH-d reactivity, while the other parts of central area were weakly
positive. Similar distribution of reactivity was expressed also in chronically inflamed
pulp; moderate to strong reaction was observed in stromal area as result of positive reaction
in inflammatory cells and endothelial cells of abundant newly formed capillaries