In Situ Synthesis of Artificial Lipids

This review comes from a themed issue on Synthetic Biomolecules (2022); Edited by Neal K. Devaraj and Shinya TsukijiFinanciado para publicación en acceso aberto: Universidade da Coruña/CISUG[Abstract] Lipids constitute one of the most enigmatic family of biological molecules. Although the importance of lipids as basic units of compartmental structure and energy storage is well-acknowledged, deciphering the biosynthesis and precise roles of specific lipid species has been challenging. To better understand the structure and function of these biomolecules, there is a burgeoning interest in developing strategies to produce noncanonical lipids in a controlled manner. This review covers recent advances in the area of in situ generation of synthetic lipids. Specifically, we report several approaches that constitute a powerful toolbox for achieving noncanonical lipid synthesis. We describe how these methodologies enable the direct construction of synthetic lipids, helping to address fundamental questions related to the cell biology of lipid biosynthesis, trafficking, and signaling. We envision that highlighting the current advances in artificial lipid synthesis will pave the way for broader interest into this emerging class of biomimetic molecules.Roberto J. Brea acknowledges support from Xunta de Galicia through the “Atracción de talento investigador” programme (ED431H2020/19). Roberto J. Brea also thanks the Agencia Estatal de Investigación (AEI) and the Ministerio de Ciencia e Innovación (MICINN) for his Ramón y Cajal contract (RYC2020-030065-I). The authors acknowledge funding for open access charge from Universidade da Coruña/Consorcio Interuniversitario de Galicia (CISUG)Xunta de Galicia; ED431H2020/1

Electrocatalytic Oxidation of Acetaminophen on a PVC/TTF-TCNQ Composite Electrode Modified by Gold Nanoparticles: Application as an Amperometric Sensor

This work shows the electrocatalytic activity towards the oxidation of acetaminophen of a composite electrode PVC/TTF-TCNQ modified with gold nanoparticles. Owing to a good linear relationship between acetaminophen concentration and current response at lower oxidation potential it is proposed as a new alternative for amperometric determination of acetaminophen. Besides the good catalytic activity (Eapp = 0.425 V), the proposed sensor shows high reproducibility, long-term stability, low cost and can be used both in batch and in FIA systems. In batch, it was obtained a linear range for acetaminophen over a range of 1 to 800 μM with a detection limit of 0.66 μM and a correlation coefficient of 0.997 with a slope (sensitivity) of 53 mA M-1. In FIA, a sensitivity of 9.9 ± 0.15 mA M-1 and a linear regression coefficient (r) of 0.999 were obtained for a linear range for acetaminophen between 1 to 800 μM. The detection limit (s/n = 3) was 8.9 μM

New Biosensor for Phenols Compounds Based on Gold Nanoparticle-Modified PVC/TTF-TCNQ Composite Electrode

A gold nanoparticle-modified PVC/TTF-TCNQ composite electrode with tyrosinase enzyme is proposed as electrochemical biosensor for detecting phenolic compounds in musts and wines samples using phenol and catechol as standards. The work potential was 0 V both in batch and in FIA system (linear range from 0.6 μM up to 10.0 μM), with a detection limit of 7.9 x 10-7 M for phenol and 6.43 x 10-7 M for catechol. The good correlation between the results obtained with the biosensor and those achieved with the Folin–Ciocalteu reference method enables that it can be used for rapid and almost automated estimation of the “total phenolics” and “high antioxidant” fraction of must and commercial wines

Electrochemical Determination of the Glyphosate Metabolite Aminomethylphosphonic Acid (AMPA) in Drinking Waters with an Electrodeposited Copper Electrode

Carbon electrodes with in situ electrodeposited copper are used to the determination of aminomethylphosphonic acid (AMPA), the major metabolite of the herbicide Glyphosate (N-(phosphonomethyl) glycine) in drinking waters. The oxidation signal to form Cu(II) ions changes after the addition of AMPA due to the formation of a complex, this effect being used to develop an analytical method simpler, inexpensive and faster than those conventionally used. Glassy carbon and carbon paste electrodes are investigated, concluding that the last is more suitable for routine analysis. Samples of drinking water fortified with different AMPA concentrations have been used to test the method

Electrodo compósito PVC/TTF-TCNQ modificado con nanopartículas de oro: aplicación para la determinación amperométrica de Paracetamol

II Encuentro sobre nanociencia y nanotecnología de investigadores y tecnólogos de la Universidad de Córdoba. NANOUC

Measurement of the neutron capture cross section of the fissile isotope 235U with the CERN n_TOF Total Absorption Calorimeter and a fission tagging based on micromegas detectors

Actual and future nuclear technologies require more accurate nuclear data on the (n, gamma) cross sections and -ratios of fissile isotopes. Their measurement presents several difficulties, mainly related to the strong fission gamma-ray background competing with the weaker gamma-ray cascades used as the experimental signature of the (n,gamma) process. A specific setup has been used at the CERN n_TOF facility in 2012 for the measurement of the (n,gamma) cross section and alpha-ratios of fissile isotopes and used for the case of the 235U isotope. The setup consists in a set of micromegas fission detectors surrounding 235U samples and placed inside the segmented BaF2 Total Absorption Calorimeter.Postprint (published version