99 research outputs found
Thermoforming of planar polymer optical waveguides for integrated optics in smart packaging materials
The innovations in smart packaging will open up a wide range of opportunities in the future. This work describes the processing of additive manufactured and planar integrated polymer optical waveguides for use in smart packaging products. The previously published combination of flexographic and Aerosol Jet printing is complemented by thermoforming and thus creates three-dimensional integrated multimode waveguides with optical attenuation of 1.9 dB/cm ± 0.1 dB/cm @ 638 nm. These properties will be the basis to develop smart applications in packaging materials
Virtual Field Studies: Conducting Studies on Public Displays in Virtual Reality
Field studies on public displays can be difficult, expensive, and time-consuming. We investigate the feasibility of using virtual reality (VR) as a test-bed to evaluate deployments of public displays. Specifically, we investigate whether results from virtual field studies, conducted in a virtual public space, would match the results from a corresponding real-world setting. We report on two empirical user studies where we compared audience behavior around a virtual public display in the virtual world to audience behavior around a real public display. We found that virtual field studies can be a powerful research tool, as in both studies we observed largely similar behavior between the settings. We discuss the opportunities, challenges, and limitations of using virtual reality to conduct field studies, and provide lessons learned from our work that can help researchers decide whether to employ VR in their research and what factors to account for if doing so
Nuclear pores as versatile reference standards for quantitative superresolution microscopy
Quantitative fluorescence and superresolution microscopy are often limited by insufficient data quality or artifacts. In this context, it is essential to have biologically relevant control samples to benchmark and optimize the quality of microscopes, labels and imaging conditions. Here, we exploit the stereotypic arrangement of proteins in the nuclear pore complex as in situ reference structures to characterize the performance of a variety of microscopy modalities. We created four genome edited cell lines in which we endogenously labeled the nucleoporin Nup96 with mEGFP, SNAP-tag, HaloTag or the photoconvertible fluorescent protein mMaple. We demonstrate their use (1) as three-dimensional resolution standards for calibration and quality control, (2) to quantify absolute labeling efficiencies and (3) as precise reference standards for molecular counting. These cell lines will enable the broader community to assess the quality of their microscopes and labels, and to perform quantitative, absolute measurements
The 2.35 year itch of Cygnus OB2 #9: III. X-ray and radio emission analysis based on 3D hydrodynamical modelling
Context. The wind-wind collision in a massive star binary system leads to the generation of high temperature shocks that emit at X-ray wavelengths and, if particle acceleration is effective, may exhibit non-thermal radio emission. Cyg OB2 is one of a small number of massive star binary systems in this class. Aims. X-ray and radio data recently acquired as part of a project to study Cyg OB2 are used to constrain physical models of the binary system, providing in-depth knowledge about the wind-wind collision and the thermal, and non-thermal, emission arising from the shocks. Methods. We use a 3D, adaptive mesh refinement simulation (including wind acceleration, radiative cooling, and the orbital motion of the stars) to model the gas dynamics of the wind-wind collision. The simulation output is used as the basis for radiative transfer calculations considering the thermal X-ray emission and the thermal/non-thermal radio emission. Results. The flow dynamics in the simulation show that wind acceleration (between the stars) is inhibited at all orbital phases by the opposing star's radiation field, reducing pre-shock velocities below terminal velocities. To obtain good agreement with the X-ray observations, our initial mass-loss rate estimates require a down-shift by a factor of ∼7.7 to 6.5 × 10-7 M yr-1 and 7.5 × 10-7 M yr-1 for the primary and secondary star, respectively. Furthermore, the low gas densities and high shock velocities in Cyg OB2 are suggestive of unequal electron and ion temperatures, and the X-ray analysis indicates that an immediately post-shock electron-ion temperature ratio of 0.1 is also required. The radio emission is dominated by non-thermal synchrotron emission. A parameter space exploration provides evidence against models assuming equipartition between magnetic and relativistic energy densities. However, fits of comparable quality can be attained with models having stark contrasts in the ratio of magnetic-to-relativistic energy densities. Both X-ray and radio lightcurves are largely insensitive to viewing angle. The variations in X-ray emission with orbital phase can be traced back to an inverse relation with binary separation and pre-shock velocity. The radio emission also scales with pre-shock velocity and binary separation, but to positive powers (i.e. not inversely). The radio models also reveal a subtle effect whereby inverse Compton cooling leads to an increase in emissivity as a result of the synchrotron characteristic frequency being significantly reduced. Finally, using the results of the radio analysis, we estimate the surface magnetic field strengths to be 0.3-52G
Variable millimetre radiation from the colliding-wind binary Cygnus OB2 #8A
Context. Massive binaries have stellar winds that collide. In the colliding-wind region, various physically interesting processes occur, leading to enhanced X-ray emission, non-thermal radio emission, as well as non-thermal X-rays and gamma-rays. Non-thermal radio emission (due to synchrotron radiation) has so far been observed at centimetre wavelengths. At millimetre wavelengths, the stellar winds and the colliding-wind region emit more thermal free-free radiation, and it is expected that any non-thermal contribution will be difficult or impossible to detect. Aims. We aim to determine if the material in the colliding-wind region contributes substantially to the observed millimetre fluxes of a colliding-wind binary. We also try to distinguish the synchrotron emission from the free-free emission. Methods. We monitored the massive binary Cyg OB2 #8A at 3 mm with the NOrthern Extended Millimeter Array (NOEMA) interferometer of the Institut de Radioastronomie Millimétrique (IRAM). The data were collected in 14 separate observing runs (in 2014 and 2016), and provide good coverage of the orbital period. Results. The observed millimetre fluxes range between 1.1 and 2.3 mJy, and show phase-locked variability, clearly indicating that a large part of the emission is due to the colliding-wind region. A simple synchrotron model gives fluxes with the correct order of magnitude, but with a maximum that is phase-shifted with respect to the observations. Qualitatively this phase shift can be explained by our neglect of orbital motion on the shape of the colliding-wind region. A model using only free-free emission results in only a slightly worse explanation of the observations. Additionally, on the map of our observations we also detect the O6.5 III star Cyg OB2 #8B, for which we determine a 3 mm flux of 0.21 ± 0.033 mJy. Conclusions. The question of whether synchrotron radiation or free-free emission dominates the millimetre fluxes of Cyg OB2 #8A remains open. More detailed modelling of this system, based on solving the hydrodynamical equations, is required to give a definite answer
Comparison of small-footprint discrete return and full waveform airborne lidar data for estimating multiple forest variables
The quantification of forest ecosystems is important for a variety of purposes, including the assessment of wildlife habitat, nutrient cycles, timber yield and fire propagation. This research assesses the estimation of forest structure, composition and deadwood variables from small-footprint airborne lidar data, both discrete return (DR) and full waveform (FW), acquired under leaf-on and leaf-off conditions. The field site, in the New Forest, UK, includes managed plantation and ancient, semi-natural, coniferous and deciduous woodland. Point clouds were rendered from the FW data through Gaussian decomposition. An area-based regression approach (using Akaike Information Criterion analysis) was employed, separately for the DR and FW data, to model 23 field-measured forest variables. A combination of plot-level height, intensity/amplitude and echo-width variables (the latter for FW lidar only) generated from both leaf-on and leaf-off point cloud data were utilised, together with individual tree crown (ITC) metrics from rasterised leaf-on height data. Statistically significant predictive models (p<0.05) were generated for all 23 forest metrics using both the DR and FW lidar datasets, with R2 values for the best fit models in the range R2=0.43-0.94 for the DR data and R2=0.28-0.97 for the FW data (with normalised RMSE values being 18%-66% and 16%-48% respectively). For all but two forest metrics the difference between the NRMSE of the best performing DR and FW models was ≤7%, and there was an even split (11:12) as to which lidar dataset (DR or FW) generated the best model per forest metric. Overall, the DR data performed better at modelling structure variables, whilst the FW data performed better at modelling composition and deadwood variables. Neither showed a clear advantage at modelling variables from a particular vegetation layer (canopy, shrub or ground). Height, intensity/amplitude, and ITC-derived crown variables were shown to be important inputs across the best performing models (DR or FW), but the additional echo-width variables available from FW point data were relatively unimportant. Of perhaps greater significance to the choice between lidar data type (i.e. DR or FW) in determining the predictive power of the best performing models was the selection of leaf-on and/or leaf-off data. Of the 23 best models, 10 contained both leaf-on and leaf-off lidar variables, whilst 11 contained only leaf-on and two only leaf-off data. We therefore conclude that although FW lidar has greater vertical profile information than DR lidar, the greater complimentary information about the entire forest canopy profile that is available from both leaf-on and leaf-off data is of more benefit to forest inventory, in general, than the selection between DR or FW lidar
Rapid characterisation of vegetation structure to predict refugia and climate change impacts across a global biodiversity hotspot
Identification of refugia is an increasingly important adaptation strategy in conservation planning under rapid anthropogenic climate change. Granite outcrops (GOs) provide extraordinary diversity, including a wide range of taxa, vegetation types and habitats in the Southwest Australian Floristic Region (SWAFR). However, poor characterization of GOs limits the capacity of conservation planning for refugia under climate change. A novel means for the rapid identification of potential refugia is presented, based on the assessment of local-scale environment and vegetation structure in a wider region. This approach was tested on GOs across the SWAFR. Airborne discrete return Light Detection And Ranging (LiDAR) data and Red Green and Blue (RGB) imagery were acquired. Vertical vegetation profiles were used to derive 54 structural classes. Structural vegetation types were described in three areas for supervised classification of a further 13 GOs across the region.Habitat descriptions based on 494 vegetation plots on and around these GOs were used to quantify relationships between environmental variables, ground cover and canopy height. The vegetation surrounding GOs is strongly related to structural vegetation types (Kappa = 0.8) and to its spatial context. Water gaining sites around GOs are characterized by taller and denser vegetation in all areas. The strong relationship between rainfall, soil-depth, and vegetation structure (R2 of 0.8–0.9) allowed comparisons of vegetation structure between current and future climate. Significant shifts in vegetation structural types were predicted and mapped for future climates. Water gaining areas below granite outcrops were identified as important putative refugia. A reduction in rainfall may be offset by the occurrence of deeper soil elsewhere on the outcrop. However, climate change interactions with fire and water table declines may render our conclusions conservative. The LiDAR-based mapping approach presented enables the integration of site-based biotic assessment with structural vegetation types for the rapid delineation and prioritization of key refugia
Innovation und Trends für Mobiles Lernen
Der Beitrag zeigt aktuelle Trends im Bereich der mobilen und ubiquitären Lerntechnologien auf, welche die klassischen Konzepte von Mobilem Lernen erweitern: a) Mobiler und allgegenwärtiger Zugang zu Lerninhalten b) unterbrechungsfreie Lernunterstützung oder "Seamless Learning Support", die nahtlose Integration von Lernunterstützung in gemischten Lernszenarien, c) Smartphones und Sensoren im Mobilen Lernen, d) Mobile Gaming und mobile Augmented Reality und e) situierte eingebettete Displays. Anhand dieser Trends werden die Konsequenzen für das didaktische Design und darunter liegende Lernkonzepte diskutiert
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