34 research outputs found
Erythrocyte hemolysis and hemoglobin oxidation promote ferric chloride-induced vascular injury,ā The
Abstract The release of redox-active iron and heme into the blood-stream is toxic to the vasculature, contributing to the development of vascular diseases. How iron induces endothelial injury remains ill defined. To investigate this, we developed a novel ex vivo perfusion chamber that enables direct analysis of the effects of FeCl3 on the vasculature. We demonstrate that FeCl3 treatment of isolated mouse aorta, perfused with whole blood, was associated with endothelial denudation, collagen exposure, and occlusive thrombus formation. Strikingly exposing vessels to FeCl3 alone, in the absence of perfused blood, was associated with only minor vascular injury. Whole blood fractionation studies revealed that FeCl3-induced vascular injury was red blood cell (erythrocyte)-dependent, requiring erythrocyte hemolysis and hemoglobin oxidation for endothelial denudation
Erythrocyte Hemolysis and Hemoglobin Oxidation Promote Ferric Chloride-induced Vascular Injury*Sā
The release of redox-active iron and heme into the blood-stream is toxic to
the vasculature, contributing to the development of vascular diseases. How
iron induces endothelial injury remains ill defined. To investigate this, we
developed a novel ex vivo perfusion chamber that enables direct
analysis of the effects of FeCl3 on the vasculature. We demonstrate
that FeCl3 treatment of isolated mouse aorta, perfused with whole
blood, was associated with endothelial denudation, collagen exposure, and
occlusive thrombus formation. Strikingly exposing vessels to FeCl3
alone, in the absence of perfused blood, was associated with only minor
vascular injury. Whole blood fractionation studies revealed that
FeCl3-induced vascular injury was red blood cell
(erythrocyte)-dependent, requiring erythrocyte hemolysis and hemoglobin
oxidation for endothelial denudation. Overall these studies define a unique
mechanism of Fe3+-induced vascular injury that has implications for
the understanding of FeCl3-dependent models of thrombosis and
vascular dysfunction associated with severe intravascular hemolysis